33 results about "Pantoea sp." patented technology

Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoaea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.

The invention discloses pantoea sp. W36-1, of which the preservation number is CGMCC No. is 3701, a flocculant prepared by the pantoea sp. W36-1 and application of the flocculant in removal of suspensions, dyeing agents and heavy metal in waste water. Extracellular products of the bacteria have an obvious absorption peak at 200 to 250nm, and polysaccharide solution can remove 54.89 percent of free groups in the bacteria. The method for preparing the pantoea sp. W36-1 comprises the following steps of: performing fermentation culture of the bacteria strain for 48 hours, centrifuging 100ml of fermentation solution at a rotation speed of 6,000r / min for 10 minutes, adding absolute ethanol, of which the volume is 1.5 times that of supernatant, into the supernatant, uniformly mixing the absoluteethanol and the supernatant standing the mixed solution at the temperature of 4 DEG C overnight, centrifuging the precipitate, washing the precipitate by 70-percent ethanol for 2 to 3 times, and thendrying the precipitate to obtain the finished product. The flocculent activity of the fermentation culture product of the pantoea sp. W36-1 is 81.7 to 83.15 percent, the yield of the pantoea sp. W36-1 is 6g / L and can be widely used for removing suspensions, dyeing agents, heavy metal and the like in waste water.

The invention provides a pantoea for converting resveratrol into epsilon-staphylin and its application, and the preservation number of the pantoea is CGMCC No.13605. The present invention also provides a method for preparing epsilon-staphylin based on the pantoea. Compared with the traditional transformation method, the pantoea provided by the present invention has high yield, no by-products, and no pollution to the environment; the strain Pantoea sp B2 of the present invention, CGMCC No.13605, has high yield and stable character The characteristics of this strain have remained stable after 20 generations of passages.

The invention discloses nitrilase N1 and its gene derived from Pantoea sp. ) nitrilase N3 and its gene, Leptolyngbya sp. nitrilase N4 and its gene, Brassica oleracea var. oleracea nitrilase N5 and its gene and Camelina sativa nitrilase N6 and its gene, and using the nitrilase as a biocatalyst to prepare p-aminomethylbenzoic acid intermediate p-cyanobenzoic acid. The resting cells of the corresponding nitrilase can catalyze 100g / L of the substrate, and the conversion rate is greater than 99%. The method has the remarkable characteristics of mild reaction conditions, no pollution, simple process route, etc., and has great industrial application prospects.

The invention discloses a rice endophyte (Pantoea sp. Sd-1) for efficiently degrading lignin and the preservation number of the Pantoea sp. Sd-1 is CGMCC No. 6698. The Pantoea sp. Sd-1 is a bacterial strain which can grow on a culture medium with alkali lignin as a unique carbon source. After the Pantoea sp. Sd-1 is cultured for four days in a liquid culture medium containing 3g / L alkali lignin and supplemented with 1% of glucose and 0.5% of peptone, the fading rate and the degradation of lignin respectively reach 52.38% and 69.06%. And in a liquid culture medium containing 10g / L rice straws and supplemented with 1% of glucose and 0.5% of peptone, the degradation of straws can reach 54.51 at most.

The invention discloses a pantoea for degrading organic phosphorus and inorganic phosphorus and application thereof. The storage number of Pantoea sp. S-32 provided by the present invention in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is CGMCC No.10017. The phosphorus solubilizing rate of the bacteria degrading calcium phosphate reaches 24.19%, which is 3.64 times that of Bacillus megaterium As1.223; It has strong environmental adaptability and can adapt to a wide range of temperature, pH value and salt concentration. Experiments have proved that Pantoea sp. S-32 CGMCC No.10017 of the present invention can be used as a strain resource for microbial fertilizers to increase crop yield and accelerate soil ecological restoration in mining areas.

The invention discloses a quinclorac-degrading bacterium and its application. A quinclorac-degrading bacterium J7 provided by the present invention belongs to Pantoea ( Pantoea sp.), was deposited in the China Center for Type Culture Collection (CCTCC), and the preservation date was March 16, 2017, and the preservation number was CCTCC M 2017131. It was identified as Pantoea by using 16S rDNA. The bacterium is a Gram-negative bacterium, and the colony is pale yellow, smooth, round, and with neat edges. The fungus has the function of degrading quinclorac, and can prevent tobacco from being injured by quinclorac.