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Pantoea with good degradation effect on cellulose

A cellulose degradation and cellulose technology, applied in the field of microbiology, can solve the problem that the degradation efficiency of degrading bacteria is not very high, and achieve the effect of good degradation effect

Active Publication Date: 2020-01-03
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, a variety of strains capable of degrading cellulose have been screened from different soils by domestic and foreign researchers, such as: Acetobacter xylinus (Feth el Zahar Haichar, et al.2007.), although most strains can degrade cellulose , however, in general, the degradation efficiency of these degrading bacteria is not very high

Method used

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  • Pantoea with good degradation effect on cellulose
  • Pantoea with good degradation effect on cellulose
  • Pantoea with good degradation effect on cellulose

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Experimental program
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Effect test

Embodiment 1

[0029] 1 Materials and methods

[0030] 1.1 Experimental Instruments

[0031]TGL-16C high-speed centrifuge (Shanghai Anting Scientific Instrument Factory), FA2004 electronic balance (Shanghai Shangping Instrument Co., Ltd.), P330-31 ultra-micro ultraviolet spectrophotometer (Implen GmbH, Munich, Germany), BOXUN vertical pressure steam Sterilization pot (Shanghai Boxun Industrial Co., Ltd.), SW-CJ-IFD ultra-clean bench (Sujing Group Suzhou Antai Air Technology Co., Ltd.), micro pipette (Biotech).

[0032] 1.2 Materials

[0033] 1.2.1 Soil samples for testing

[0034] The soil samples used in this experiment were collected from permafrost (0-15 cm) collected in Mohe County, Daxing'an Mountains, Heilongjiang Province. The soil samples were passed through a 20-mesh sieve to remove stones and plant debris, and then stored in sealed bags at 4°C.

[0035] 1.2.2 Reagents

[0036] 3,5-Dinitrosalicylic acid was purchased from Beijing Biolite Technology Co., Ltd., Congo Red was purc...

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Abstract

The invention discloses pantoea with a good degradation effect on cellulose, and belongs to the technical field of microbiology. The pantoea is characterized in that a strain B5-1 is isolated from frozen soil collected from the Mohe county of the Daxinganling region of the Heilongjiang province by using an enrichment culture method, and the strain B5-1 can fully degrade straw with thickness of 10cm within 72 days; the strain B5-1 is directly isolated by a sodium carboxymethylcellulose slab, through physiological, biochemical and 16S rDNA identification, the strain B5-1 is identified as the Pantoea, and the preservation date of the strain is November 30, 2018; the preservation number is CGMCC No.16848; and degradation experiments show that the strain B5-1 has a good degradation effect on the cellulose.

Description

technical field [0001] The invention relates to a strain of pantoea with good degrading effect on cellulose, belonging to the technical field of microbiology. Background technique [0002] Cellulose is a linear glucan formed by the linkage of glucose residues by β-1,4-glycosidic bonds, which widely exists in plants. Corn stover is an agricultural waste with huge biomass in the world. It is rich in cellulose components and can play an important role in many fields. However, the current utilization situation has resulted in a considerable part of corn stalks being used with low value and discarded directly, resulting in a waste of resources and pollution of the environment. Cellulose is the most widespread type of carbohydrate in nature, and it is also the largest renewable resource on earth. At present, only a small part of cellulose in nature has been utilized, and the vast majority of cellulose is not only wasted, but also causes environmental pollution. Using microorgan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12R2001/01C12N1/205
Inventor 臧淑英柳鑫鹏智刚马欣然王翊婷张鑫渠凤甜谢桂林解瑞峰
Owner HARBIN NORMAL UNIVERSITY
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