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Composite microbial agent and application thereof in biogas residue high-temperature aerobic composting

A technology of compound microbial inoculants and bacilli, applied in the field of environmental microorganisms, can solve the problems of low composting temperature, long composting cycle, incomplete decomposing of organic matter, etc., so as to improve the decomposing degree of materials and the quality of organic fertilizer, rapidly decompose and shorten the composting period. effect of time

Active Publication Date: 2022-05-13
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, composting technology still has problems such as long composting cycle, low compost temperature, and incomplete decomposition of organic matter.

Method used

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  • Composite microbial agent and application thereof in biogas residue high-temperature aerobic composting
  • Composite microbial agent and application thereof in biogas residue high-temperature aerobic composting
  • Composite microbial agent and application thereof in biogas residue high-temperature aerobic composting

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preparation example Construction

[0031] The present invention also provides a preparation method of the liquid composite microbial bacterial agent, the preparation method comprising the following steps: making Lithuanian Geobacillus lituanicus G6-1, Pitt Acinetobacter Acinetobacterpittii AP-16, Pantoea sp.BK - mixing PP311 and fermented broth of Geobacillus kaustophilus FWGK-JYJ1 to obtain the liquid composite microbial agent.

[0032] In one embodiment, the Lithuanian Geobacillus lituanicus G6-1, Pitt Acinetobacter Acinetobacter pittii AP-16, Pantoea sp.BK-PP311 and thermophilic Geobacillus kaustophilus FWGK-JYJ1 The fermentation broths were all prepared by the following method: Lithuanian Geobacillus lituanicus G6-1, Pitt Acinetobacter pittii AP-16, Pantoea sp.BK-PP311 and thermophilic Geobacillus kaustophilus FWGK-JYJ1 The pure strains are inoculated in the liquid medium for cultivation, and after the cultivation is completed, it is the fermentation liquid of each strain.

[0033] In one embodiment, after...

Embodiment 1

[0080] Embodiment 1: the preparation of composite microbial bacterial agent

[0081] Preparation of liquid composite microbial agent: Geobacillus lituanicus G6-1, Acinetobacter pittii AP-16, Pantoea sp.BK-PP311, Geobacillus kaustophilus FWGK-JYJ1 The pure strains of the strains were inoculated into 5mL CYS liquid medium, and cultured at 50°C and 180r / min for 12-16h; the inoculation amount of 1% by volume was inserted into the same CYS liquid medium for gradual After expanding the culture, after the cultivation under the same conditions, the fermentation broths of the four strains were obtained respectively; the fermentation broths of the four strains were evenly mixed according to the volume ratio of 1:1:1:1 to obtain liquid composite microorganisms Bacteria.

[0082] The composition of CYS liquid medium is: peptone 3g / L, yeast extract 2g / L, soluble starch 1g / L, NaCl 3g / L, MgCl 2 0.125g / L, CaCl 2 0.5g / L, FeSO 4 ·7H 2 O 0.01g / L, trace element solution (Na 2 MoO 4 12g / ...

Embodiment 2

[0084] Example 2: Effect of compound microbial agent on high-temperature aerobic composting of biogas residue and rice straw

[0085] Take 10kg of biogas residue from anaerobic fermentation of kitchen waste and 3kg of rice straw, put them in aerobic fermentation equipment with good thermal insulation performance, and mix them evenly to obtain a total of 13kg of mixed materials. Add 65g of solid composite microbial bacterial agents according to the mass ratio of 0.5%. Stir well. At this time, the initial C / N of the material is 26, the initial moisture content of the material is adjusted to 60%, and the initial pH value is 7.0. Then start the aerobic fermentation of the material, with uninterrupted ventilation during the process, and stir the material once a day. At the same time, set up a control group without adding compound microbial agents, and use the same materials and conditions for aerobic composting. At the same time every day, the compost temperature was measured and...

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Abstract

The invention relates to the technical field of environmental microorganisms, in particular to a compound microbial agent and application thereof in high-temperature aerobic composting of biogas residues. The compound microbial agent is prepared from the following components: Geobacillus variotii G6-1, Acinetobacter piteti pittii AP-16, Pantoea sp. BK-PP311 (Pantoea sp. BK-PP311), and Geobacillus kaustophilus FWGK-JYJ1 (Bacillus kaustophilus FWGK-JYJ1), and the compound microbial agent is prepared from the following raw materials: Geobacter variotii G6-1, Acinetobacter piteti pittii AP-16, Pantoea sp. BK-PP311, Geobacter kaustophilus FWGK-JYJ1, Pantoea sp. The compound microbial agent is used for high-temperature aerobic composting of biogas residues, can improve the highest temperature of a compost body, prolong the high-temperature period, quickly decompose organic matters, shorten the composting time and improve the decomposition degree of materials and the quality of organic fertilizers, and has relatively high application value.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a composite microbial bacterial agent and its application in biogas residue high-temperature aerobic composting. Background technique [0002] With the advancement of waste classification work, the output of wet waste in my country is increasing day by day. At present, the mainstream treatment technology of wet waste is anaerobic digestion. However, anaerobic digestion will produce a large amount of by-products - biogas residues, about 25 kilograms of biogas residues will be produced for every ton of wet garbage processed. Biogas residue treatment technology is mainly dry incineration. Although incineration alleviates the burden of a large amount of biogas residues produced by anaerobic digestion of wet garbage to a certain extent, incineration will not only cause energy loss and waste of resources, but also convert organic carbon in biogas residues into ca...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F9/04C12R1/01
CPCC12N1/20C05F9/04Y02A40/20Y02W30/40
Inventor 刘莉赵玥史吉平吉雨霁王娜杨宁王芷
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
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