64 results about "Pantoea agglomerans" patented technology

Microbiological agents are provided for control of certain diseases of wheat and other cereals caused by Fusarium species, including Fusarium head blight of wheat and other cereals. These agents can also improve yield of wheat plants and cereals. The agents are novel isolates of Pantoea agglomerans and of Bacillus megaterium that exhibit the property of inhibiting fungal pathogens, particularly those produced by Fusarium species. Biocontrol compositions, and methods of using them to control plant pathogen development on wheat and cereal plants and for increasing plant yield, are also provided. The biocontrol compositions comprise a mixture of at least one microorganism selected from the group consisting of Pantoea agglomerans and Bacillus megaterium.

Six bacterial strains: Bacillus simplex strain 03WN13, Bacillus simplex strain 03WN23, Bacillus simplex strain 03WN25, Pseudomonas koreensis strain 10IL21, Pantoea agglomerans strain 10IL31, and Pseudomonas lini strain 13IL01, are superior antagonists of Phytophthora erythroseptica Pethybr., the causative agent of pink rot on potatoes. These bacterial strains are effective for suppression and control of pink rot on potatoes.

The invention provides efficient phosphorus-dissolution promotion bacteria and a microbial agent prepared from the same. The effective components of the microbial agent include Pantoea agglomerans P36 and Trichoderma harzianum. In the invention, the widely-adaptable efficient phosphorus-dissolution yield-increasing bacteria Pantoea agglomerans P36 and the efficient anti-crop disease Trichoderma harzianum are organically combined; by applying the composite bacterial agent provided by the invention, the effect of the microorganism in preventing soil-borne diseases is sufficiently exerted while efficiently utilizing the soil phosphorus and improving the utilization rate of phosphate fertilizer; an effect of increasing yield by dissolving phosphorus and resisting diseases is realized; and theinvention has tremendous application values in agriculture.

A biological inoculant for enhancing the growth of plants is disclosed. The inoculant includes the bacterial strains Herbaspirillum seropedicae 2A, Pantoea agglomerans P101, Pantoea agglomerans P102, Klebsiella pneumoniae 342, Klebsiella pneumoniae zmvsy, Herbaspirillum seropedicae Z152, Gluconacetobacter diazotrophicus PA15, with or without a carrier. The inoculant also includes strains of the bacterium Pantoea agglomerans and K. pneumoniae which are able to enhance the growth of cereal grasses. Also disclosed are the novel bacterial strains Herbaspirillum seropedicae 2A, Pantoea agglomerans P101 and P102, and Klebsiella pneumoniae 342 and zmvsy.

The invention relates to a microbial fertilizer and a preparation method thereof. The preparation method is as follows: bacillus cereus, bacillus megaterium and bacillus subtillis are mixed and fermented; bacillus mucilaginosus, pantoea agglomerans and azotobacter chroococcum are fermented respectively; the four fermented cultures are mixed and filled according to a certain proportion so as to produce a microbial blend which is used by the methods of seed soaking, root irrigation and spraying. The microbial fertilizer can promote plant rhizosphere growth and propagation, improve soil, eliminate soil hardening, enhance soil permeability and promote the development of the root system; the microbial fertilizer has the advantages of releasing potassium and phosphorus, fixing nitrogen, improving fertilizer efficiency and reducing the amount of chemical fertilizer used, inhibiting the propagation of harmful bacteria in the soil, preventing the diseases such as soil-borne diseases, replant disease and the like; thus realizing the functions of increasing income and improving the quality of agricultural products.

The invention discloses Pantoea agglomerans B50l CGMCC No.1655 and fermenting cultivating method and application. The method includes the following steps: inoculating the Pantoea agglomerans B50l CGMCC No.1655 into triangle bottle to gain seed liquid, inoculating the seed liquid into fermenting jar special cultivating medium for 48-96 hours. It has good effect to avoid disease, high effect efficiency, high biology safety and simple manufacture technology.

The invention discloses a compound microbial agent for promoting fermentation of pig manure composting. The compound microbial agent is prepared from thermobifida fusca, ureibacillus thermophaericus, pantoea agglomerans, gibsonii, bacillus amyloliquefaciens, bacillus pumilus and a liquid culture medium. Compared with other treatment methods, the fermentation time of the compound microbial agent on the pig manure composting is shorter, and more obvious facilitation effect on fermentation of the pig manure composting is obtained.

The invention provides a culture medium prepared from peptone, yeast and NaCl, wherein strains cultured in the culture medium are pantoea agglomerans belonging to IMH cells. After being collected, thallus cells are dispersed to different cultivation systems according to the difference among nano-particles required to be synthesized, metal ions are added to meet the requirement of a proper concentration, reactions are carried out for several hours, and separation and collection are carried out according to different reaction times, so as to obtain metal nano-particle materials of different particle diameters. The whole process is environmentally friendly, simple and convenient to operate, and low in cost; the obtained nano-particle materials have wide application prospects in the aspects of catalysis, adsorption, detection and the like.

For the purpose of providing a method of safely and inexpensively producing a fermented plant extract containing an immunopotentiator at a high concentration, the method for fermentation and culture of the present invention ferments a plant component such as wheat flour using Pantoea agglomerans which is a gram negative bacterium which lives in a symbiotic relationship with a plant such as wheat and apple. It becomes possible to remarkably augment an immunopotentiation action which the plant has. In addition, these are not contaminated with impurities derived from animal components, and thus these are highly safe.

The invention relates to a Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and an application, which belongs to the biology technical field. The Pantoea agglomerans1-7 is preserved in the China General Microbiological Culture Collection Center on May 28th, 2012 with the preservation number of CGMCC No.6160. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 is used for preparing the preparations used for preventing and treating bacterial soft rot of konjak and clubroot disease on cruciferous vegetable club root in fields. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 has the advantages of high efficiency, no toxicity, safety and no residue, the bacterial strain1-7 has the characteristics of good disease control, better growth promotion effect, better comprehensive characters and easy industrial production, and the Pantoea agglomerans1-7 can be applied to preparation of preparations for controlling cruciferous vegetable club root in fields.

The invention discloses a microbial fertilizer, comprising 10-30 percent of Bacillus cereus culture, 10-30 percent of Bacillus subtilis culture, 10-30 percent of Pantoea agglomerans culture, 10-30 percent of Lactobacillus plantarum culture and 10-30 percent of Lactobacillus acidophilus culture. The microbial fertilizer can be used for soaking seeds, irrigating roots and spraying or can be used for fermenting livestock manure and a pulverized straw mixture. The microbial fertilizer also can be prepared by mixing the liquid inoculants with a well-known carrier and then drying to form the solid microbial fertilizer which is convenient to store and transport. The carrier can be bran, crop straw or other carriers suitable to be used as fertilizers.

The invention discloses a complex microbial agent for low-temperature methane fermentation. According to the ccomplex microbial agent, fermentation broths obtained by fermenting pseudomonas sp. CGMCC (China General Microbiological Culture Collection) No.4764, pantoea agglomerans CGMCC No.4765 and pantoea agglomerans are uniformly mixed according to the weight ratio of 1:1:1, so that a liquid formis obtained. The complex microbial agent disclosed by the invention can be directly fed into a methane tank, so that the methane tank can be used for normally producing gas in winter; and in addition, the complex microbial agent has a wide application value.

The invention relates to a method for preparing a copper selenide nano-material by using Pantoea agglomerans. The copper selenide nano-material with three different stoichiometric ratios is prepared by adjusting the amount of a chemical reducing agent NaBH4 in a solution. When a ratio of n(EDTA-Cu):n(NaBH4) in a working medium solution is not greater than 4-8:1, the product is a Cu2Se nanosphere, and belongs to a tetragonal system, the primary sediment size is 20nm, and the secondary sediment size is 50-100nm; when the ratio of n(EDTA-Cu):n(NaBH4) in a working medium solution is greater than 4-8:1, the product is a CuSe nanosphere, and belongs to a hexagonal system, and the particle size is 100-200nm; and when the solution does not contain NaBH4, the product is a Cu2-xSe nanosphere, and belongs to a cubic system, and the particle size is uniform and is about 80nm. The problems of high cost, large energy consumption and environmental pollution existing in present copper selenide preparation methods are solved in the invention.

Provided is a lyoprotected microcapsule for increasing the survival of a microorganism, such as Pantoea agglomerans E325, after lyophilization and / or storage, which includes an interior core having at least one live microorganism; a first polymer; and at least one nutrient, as well as an exterior shell having second polymer. The lyoprotected microcapsule also includes at least one lyoprotective agent. The polymer may include alginate and the lyoprotective agent may include maltodextrin, trehalose and combinations thereof. Microorganisms within the lyoprotected microcapsules exhibit enhanced survival after lyophilization and / or storage. Also provided are methods for producing such lyoprotected microcapsules and methods for using the lyoprotected microcapsules.

The invention relates to a biocontrol strain JH21 for controlling rice sheath blight and rice blast, belonging to the technical field of plant protection. The bacteria are Pantoea agglomerans. The preparation method of the bacterial agent thereof comprises the following steps: using the bacteria to perform shaking cultivation in potato dextrose agar(PDA) culture solution at 30 DEG C in 180rpm for20-24 hours, then centrifuging in 6000rpm for 10 minutes, and using wet bacteria and the preservative fluid of the laboratory to prepare the bacterial agent according to a ratio of 1:40 (g / ml), wherein the total concentration of the live bacteria in the finished product is 3*10<9>-3*10<12>CFU / ml. The control rate of the JH21 bacterial agent to rice blast is about 65-75%, the yield can be increased by 40-50%; and the control rate to rice sheath blight is more than 60-70% and the yield can be increased by 40-60%. The JH21 bacterial agent can control rice sheath blight and rice blast simultaneously in one field; and the bacterial agent can obviously promote the growth of rice and increase the yield of rice. The bacterial agent can be used through soaking during seeding and after rice is transplanted, the bacterial agent is sprayed to control diseases.

The present invention belongs to the technical fields of agriculture and environmental pollution treatment, and relates to endophytic bacteria capable of producing ACCD, its soil renovation bacterium agent and an application thereof. The endophytic bacteria Jp3-3 which is named as Pantoea agglomerans Jp3-3 and preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is 13th July, 2011, and the collection number of CCTCC is No: M 2011250. The ACCD enzymatic activity of the bacteria can reach as high as 402umM alpha-KB.h<-1>.mg<-1>; a liquid preparation contains effective live bacteria with amount of more than 1 billion / milliliter, a solid preparation contains live bacteria with amount of more than 0.2 billion / gram. The direct application of the soil renovation bacterium agent is capable of promoting the growth of the plants, absorbing the heavy metal and reinforcing the efficiency of plants for restoring the soil polluted by the heavy metal.

The invention discloses a pantoea agglomerans strain and application thereof. The pantoea agglomerans strain is named as Pantoea agglomerans ZJU23, with a preservation number of CGMCC No.16174 and a preservation date of July 30, 2018. According to the pantoea agglomerans strain, the control effect on wheat scab is up to 50-70%, vomitoxin is reduced by 50-80%, diseases caused by plant pathogenic fungi such as gibberellic disease, gray mold, rice blast, colletotrichum destructivum and rice sheath blight disease can also be simultaneously controlled, and the range of controlling is wide.

The invention relates to a method for resource utilization of domestic garbage. The method comprises the following steps of crushing the domestic garbage and sifting the crushed domestic garbage with a 5mm sieve; adjusting the moisture content of sifted domestic garbage particulate matters in the range of 20%-25% and the pH value in the range of 7-9; adding a microbial inoculum consisting one or more of white rot fungi, bacillus, staphylococcus or pantoea agglomerans to the domestic garbage particulate matters; and fermenting for 5-7 days in an anaerobic environment at 30-50 DEG C; adding 15%-20% of granite stone powder, 10%-15% of cement to 70% of the fermented domestic garbage particulate matters according to the mass ratio; mixing and stirring for 5-10min; carrying out compaction-molding in a grinding tool under the action of 1MPa; and maintaining for 28 days at the temperature of 20 + / - 2 DEG C and at the humidity of 95% to obtain a domestic garbage building material. The material achieves the purpose of resource utilization of the domestic garbage and the granite stone powder and has the characteristics of high strength, light weight, slight pollution, low price and convenience in preparation.

The invention discloses a rhodobacter sphaeroides engineering strain for producing beta-carotene and a construction method thereof. The method includes first optimizing a codon of a lycopene cyclase gene crtYPa derived from pantoea agglomerans to obtain an opt crtYPa gene, then replacing an endogenous phytoene three-step dehydrogenase gene crtI3 of rhodobacter sphaeroides with the opt crtYPa genecontaining a promoter prrnB and a phytoene four-step dehydrogenase gene crtIPa gene derived from the pantoea agglomerans in a traceless mode, then knocking out an endogenous neurosporene hydroxylase gene crtC of the rhodobacter sphaeroides and a 6-phosphoglucose dehydrogenase gene zwf and finally knocking out an endogenous 1-deoxyxylulose-5-phosphate synthase gene dxs of the rhodobacter sphaeroides with integration expression at zwf position to obtain the rhodobacter sphaeroides engineering strain for producing the beta-carotene. The strain is cultured by fermentation, and the content of the beta-carotene can reach 30mg / g DCW.

The invention provides pantoea agglomerans and application thereof. A preservation number of the pantoea agglomerans is CCTCC NO: M2015563. The thallus of the pantoea agglomerans is rod-shaped and is negative when being treated by Gram staining; the pantoea agglomerans can be prepared into a liquid bacterial agent or wettable powder and is used for preventing and treating wheat scab and reducing the content of wheat gibberellin; in a process of solving toxin pollution, no toxic byproducts are generated and secondary pollution is not caused, so that the quality of agricultural products is improved and the edible safety of people and livestock is ensured.

The invention relates to the field of seed science, in particular to preparation and application methods of a salt-tolerant biological stimulin capable of improving germination of corn seeds. An important strain Pantoea agglomerans strain HX-1 is obtained from root systems of a Qingdao Laoshan agastache rugosus plant by separation, screening, purification and identification, research finds that the strain has indoleacetic acid producing, phosphate solubilizing and salt tolerance capacity, the vigor of corn seeds can be effectively improved after being soaked by a bacterial solution of the strain, and the biological stimulin prepared with a strain Bacillus megaterium HX-2 can effectively improve the germination and seedling emergence capacity of corn seeds in saline and alkaline land. On the basis that the biological stimulin can have a stimulation effect on the natural process of plants and particularly have effects of improving abiotic stress resistance and the like, the strain HX-1 is successfully obtained from root systems of the agastache rugosus plant and applied to study of preparation and application methods of the target biological stimulin. The biological stimulin can be widely applied to production of corn in saline and alkaline land.

The invention discloses a phenylalanine amino mutase mutant derived from pantoea agglomerans, and belongs to the technical field of enzyme engineering. The sequence of the phenylalanine amino mutase mutant is shown in SEQ ID NO.2, and the mutant enzyme still has 60% residual enzyme activity after being processed at 50 DEG C for one hour and has higher temperature stability compared with wild enzyme. Meanwhile, the mutant also has better pH stability, and is good for future industrial production.

The invention discloses a multi-heavy-metal-resistant strain for producing indoleacetic acid and application of the multi-heavy-metal-resistant strain. The pantoea agglomerans CDL29 was deposited in the China General Microbiological Culture Collection Center with the accession number of CGMCC No. 15523 on March, 28, 2018. The strain can growth under various heavy metal concentrations (high-concentration copper, zinc, lead and zinc), the yield of the indoleacetic acid is high, and after the strain is inoculated in heavy metal polluted soil, growth of wheat and development of a root system can be promoted effectively.

The invention discloses a pantoea agglomerans and application thereof, and also discloses a screening method of the pantoea agglomerans; the invention obtains the pantoea agglomerans (MFC3) by separation and purification from the soil sample of an ancient forest of Zhaoqing in Guangdong, which is preserved in China culture collection and management committee general microorganisms center, with the preservation number of CGMCC2453; the invention has stronger reducing capacity of Fe(III) or humic acid and has the advantages that the electronic utilization spectrum is wide, the iron oxides with high crystallinity can be reduced, the screening method is simple, and has the possibility of industrial production.

The invention discloses solid composite accelerant for low-temperature methane and a preparation method thereof. The preparation method includes selecting Bacillus pumilus CICC10440, Streptococcus lactis CICC20711, Pseudomonas sp. CGMCC No. 4764 and Pantoea agglomerans CGMCC No. 4765, performing purification and rejuvenation to each of the four bacteria to obtain fresh pure culture fermentation liquors, mixing the four fermentation liquors according to a mass ratio of 1:1:1:1, well mixing the mixed liquor with soybean meal and bran which are equal in mass of the mixed liquor, spreading mixture in a shady and dry environment for drying in shade, adding 6-36% of ferric trichloride and 1.2-7.2% of nickel chloride, and well mixing to obtain the solid composite accelerant, wherein the soybean meal and the bran are mixed according to a weight ratio of 1:1. The solid composite accelerant is applicable to low-temperature methane and is worthy of wide application.

The invention provides a compound microbial agent for reducing ammonia gas release in chicken manure composting and a preparation method and application thereof, and belongs to the technical field ofmicroorganisms. The compound microbial agent consists of Pantoea agglomerans HAAS-1, Anoxybacillus rupiensis HAAS-2 and Enterobacter aerogenes HAAS-3. The compound microbial agent can effectively reduce the ammonia gas yield in the chicken manure composting process, and when the compound microbial agent is added in an amount of 2.5% of the composting material weight, the total release amount of ammonia gas can be reduced by 65% or above in the first 15 days of composting fermentation; and the compound microbial agent is used for ammonia gas removal of the chicken manure compost, and is simpleto use, low in cost and free from secondary pollution.

The invention relates to a microorganism aerobiotically reducing arsenic high-effectively, which is separated from a high-arsenic pollution region in Inner Mongolia, China. 16S rDNA identification proves that the microorganism belongs to pantoea agglomerans and is named Pantoea sp.IMH. A bacterial strain of the microorganism is assigned the accession number CGMCC No.8825. The microorganism is wide in pH and temperature ranges of growth, is very high in tolerance on trivalent arsenic and pentavalent arsenic, and can reduce more than 90% of the pentavalent arsenic in 36 h under an aerobiotical culture condition.