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Pantoea agglomerans strain and application thereof

A technology of Pantoea agglomerans and Pantoea agglomerans, which is applied in the directions of application, bacteria, fungicides, etc., to achieve a broad-spectrum effect of control

Active Publication Date: 2020-01-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there are no reports on the use of Pantoea agglomerans as biocontrol bacteria to simultaneously control plant pathogenic fungal diseases and mycotoxins such as scab, gray mold, rice blast, rice sheath blight, soybean anthracnose, etc.

Method used

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  • Pantoea agglomerans strain and application thereof
  • Pantoea agglomerans strain and application thereof
  • Pantoea agglomerans strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of biocontrol bacteria containing ZJU23 strain

[0033] 1. Acquisition of ZJU23 strain

[0034] Pantoea agglomerans ZJU23, Gram stain negative, no spores, unipolar flagella, able to move. A 1.2mm colony can be formed after culturing on LB medium for 24 hours. The colony can produce yellow pigment, round, convex surface, smooth, thicker, easy to provoke, and neat edges (see figure 1 A). The 16S rDNA sequence is shown in the attachment, and the result of comparison at NCBI shows that it is Pantoea agglomerans (Pantoea agglomerans).

[0035] The above-mentioned Pantoea agglomerans was deposited by the General Microbiology Center of the China Microbial Species Collection Management Committee on July 30, 2018, and the deposit number is CGMCC No. 16174, so it is named Pantoea agglomerans. ZJU23 CGMCC No. 16174, referred to as ZJU23 strain.

[0036] 2. Preparation of biocontrol agent

[0037] 1) Inoculate the ZJU23 strain into LB culture medium, culture at 30°C,...

Embodiment 2

[0041] Example 2 Antagonistic activity of ZJU23 strain against Fusarium graminearum

[0042] 1. Determination of plate antagonistic activity of strain ZJU23 against Fusarium graminearum

[0043] Use the confrontation culture method to inoculate the Fusarium graminearum PH-1 (model bacteria) stored at 4°C on the PDA plate for activation. After the fungus is overgrown on the plate, use a sterile puncher to evenly pound from the outer edge of the colony. Into a circular clump with a diameter of 6mm. Inoculate the mycelium block in the center of WA plate (WA medium / L: peptone 5g, glucose 10g, gravy extract 3g, sodium chloride 5g, agar 20g, pH=7.0) center, about 25mm away from the center on its circumference ZJU-23 was inoculated, and the experiment took clear water as the control group. Cultivate at 25°C, and record the size of the inhibition zone after the mycelium grows over the plate.

[0044] The activity of ZJU-23 against Fusarium graminearum was evaluated according to the size o...

Embodiment 3

[0065] Example 3 The control effect of ZJU23 strain on wheat head blight and its vomiting toxin

[0066] 1. Evaluation of the effect of ZJU-23 strain in the light incubator on controlling wheat head blight

[0067] Collect wheat ears in blooming stage, spray and inoculate 10 8 The ZJU-23 strain with cfu / mL (containing 0.05% Tween20) until the water droplets flow (about 5 mL per ear), and then placed in a light incubator at 25°C for 24 hours. The next day, Fusarium graminearum PH-1 spore suspension (10 5 A / mL, containing 0.05% Tween20) was sprayed to inoculate wheat ears (sprayed to form small water droplets, about 4 mL per ear), and then placed in a light incubator at 25°C and 90% humidity to observe the disease. The experiment set up two treatment groups: ZJU23 and clear water control. Each treatment group was inoculated with 15 wheat ears, and cultivated after inoculation until the whole ear of wheat ears in the clear water treatment group was onset (see Figure 5 ), count the n...

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Abstract

The invention discloses a pantoea agglomerans strain and application thereof. The pantoea agglomerans strain is named as Pantoea agglomerans ZJU23, with a preservation number of CGMCC No.16174 and a preservation date of July 30, 2018. According to the pantoea agglomerans strain, the control effect on wheat scab is up to 50-70%, vomitoxin is reduced by 50-80%, diseases caused by plant pathogenic fungi such as gibberellic disease, gray mold, rice blast, colletotrichum destructivum and rice sheath blight disease can also be simultaneously controlled, and the range of controlling is wide.

Description

Technical field [0001] The invention relates to the technical field of development and utilization of microbial germplasm resources, in particular to a strain of Pantoea agglomerans and its application. Background technique [0002] Wheat is the staple food for nearly half of the population in our country. Ensuring the production and quality of wheat is directly related to the safety of our country's grain and food. In recent years, wheat head blight caused by a complex population of Fusarium graminearium as the dominant species has become one of the most important diseases affecting wheat production in my country. Wheat scab in general epidemic years can cause 10%-30% yield loss, and pandemic years can even lead to field failure. At the same time, when the bacteria infect wheat, they will produce vomiting toxin (Deoxynivalenol, deoxynivalenol), zearalenone and other mycotoxins. Among them, vomitin is the mycotoxin with the highest pollution rate and pollution level in wheat an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 陈云马忠华徐孙德尹燕妮
Owner ZHEJIANG UNIV
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