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Biocontrol strain JH21 for controlling rice sheath blight and rice blast and biocontrol bacterial agent thereof

A technology of rice sheath blight, JH21, which is applied in the fields of biocontrol strain JH21 and its biocontrol fungicide, and the prevention and control of rice blast and rice sheath blight in major rice production areas, and can solve the problems of high cost, high pesticide residues, and rice sheath blight. Problems such as blast and rice sheath blight are difficult to control, so as to achieve the effect of low price, saving expenses, and benefiting the pollution-free production of rice

Inactive Publication Date: 2011-09-21
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0007] The purpose of the present invention is to address the problems of rice blast and rice sheath blight prevention and control in current agricultural production, which are difficult, high in cost, increasingly prominent in chemical pesticide resistance, and high in pesticide residues. Screen high-efficiency biocontrol strains and prepare biocontrol agents, which are effective against rice blast and rice sheath blight

Method used

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  • Biocontrol strain JH21 for controlling rice sheath blight and rice blast and biocontrol bacterial agent thereof
  • Biocontrol strain JH21 for controlling rice sheath blight and rice blast and biocontrol bacterial agent thereof
  • Biocontrol strain JH21 for controlling rice sheath blight and rice blast and biocontrol bacterial agent thereof

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Embodiment Construction

[0019] A biocontrol strain JH21 screened from rice rhizosphere soil to control rice sheath blight and rice blast, the bacteria is Pantoea ( Pantoea agglomerans ), a single colony was produced on LB medium for 8 hours, and after 24 hours of cultivation, the colony was yellow, uniform in texture, round to irregular, wet, with a raised center, smooth surface, and the cells of the bacteria were straight rods, G - .

[0020] The preparation method of JH21 microbial agent adopts conventional bacterial culture method:

[0021] 1) Inoculate the strain into PDA culture solution and culture at 30°C and 180rpm respectively. After 16 to 20 hours, take samples and measure the OD value at 600nm in the ultra-clean workbench. During the measurement process, use the uninoculated culture solution to adjust to zero;

[0022] 2) When the OD value is not between 0.1 and 1, continue to cultivate each bacteria, and measure it every 3 hours until each OD value is within this range. When the OD valu...

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Abstract

The invention relates to a biocontrol strain JH21 for controlling rice sheath blight and rice blast, belonging to the technical field of plant protection. The bacteria are Pantoea agglomerans. The preparation method of the bacterial agent thereof comprises the following steps: using the bacteria to perform shaking cultivation in potato dextrose agar(PDA) culture solution at 30 DEG C in 180rpm for20-24 hours, then centrifuging in 6000rpm for 10 minutes, and using wet bacteria and the preservative fluid of the laboratory to prepare the bacterial agent according to a ratio of 1:40 (g / ml), wherein the total concentration of the live bacteria in the finished product is 3*10<9>-3*10<12>CFU / ml. The control rate of the JH21 bacterial agent to rice blast is about 65-75%, the yield can be increased by 40-50%; and the control rate to rice sheath blight is more than 60-70% and the yield can be increased by 40-60%. The JH21 bacterial agent can control rice sheath blight and rice blast simultaneously in one field; and the bacterial agent can obviously promote the growth of rice and increase the yield of rice. The bacterial agent can be used through soaking during seeding and after rice is transplanted, the bacterial agent is sprayed to control diseases.

Description

1. Technical field [0001] The invention relates to a biocontrol bacterial strain JH21 for preventing and treating rice sheath blight and rice blast and its biocontrol agent, which belongs to the technical field of crop disease prevention and treatment, and is specially used for the prevention and treatment of rice blast and rice sheath blight in major rice production areas . 2. Background technology [0002] Rice is one of the main food crops in my country. The current planting area accounts for about 1 / 4 of the country's cultivated land area, and its annual output is about 1 / 2 of the country's total grain output. However, the continuous occurrence of rice diseases has been seriously affecting rice production. Under the current control conditions, the national average annual rice production reduction due to various rice diseases reaches 20 billion kg, and if no control is carried out, the production reduction can reach 30 billion kg. Therefore, it is urgent to study and co...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCY02A40/10
Inventor 郭坚华周冬梅张文芝陈云
Owner NANJING AGRICULTURAL UNIVERSITY
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