The invention discloses a culture method for sturgeon in flowing water. A formula for an artificial feed comprises the following components in percentages: 45% of fish meal, 15% of feed yeast, 15% ofbean cakes, 5% of maize meal, 5% of dry milk, 3% of mixed salt, 2% of amino acid, 2% of an adhesive and 5% of an attractant. According to the invention, a correct sturgeon culturing method creates a good living environment for the sturgeon, and the quality of water is guaranteed; bait and sturgeon feces in the interior of a pool are timely cleaned; and a scientific formula of the artificial feed provides good feeding bait for the growth of the sturgeon. Diseases and pests are prevented, and different measures are taken for different diseases during occurrence of diseases and insect pests, wherein (1) enteritis disease is treated mainly through antibiotics, (2) saprolegniasis is treated through an immersion bath of a table salt solution with a concentration of 0.05% for 20 min, and (3) ichthyophthiriasis is treated through soaking in a formaldehyde solution with a concentration of 0.05% for 30 to 60 min. Good survival of the sturgeon is guaranteed, and healthy culturing of the sturgeonis promoted. An EM bacterial liquid is used, so the quality of the water can be remained to be clarified; the quality of the water is prevented from aging; the growth of plankton is promoted; and diseases of the sturgeon are prevented from occurrence.