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Application of curcumin to preparation of drug for killing fish epizoic infusorians

A technology of curcumin and ciliates, which is applied to the application field of curcumin in the preparation of drugs for killing fish ectoparasitic ciliates, which can solve problems such as economic losses, great harm to human health, and high residues of chemical drugs, and achieve broad application prospects , Abundant raw materials, low price effect

Inactive Publication Date: 2016-09-21
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The multi-spored melon worm parasitizes in the epithelial tissue and gill filament tissue of various fishes, and strips the epithelial cells and red blood cells of the host, often causing thousands of fish species to die within a few days, causing serious economic damage. loss
For decades, chemical medicines have become the most effective way to prevent and control the disease of small melon worms, but these chemical medicines have side effects such as high residues, environmental pollution, and great harm to human health, such as malachite green and mercury preparations. It has been reported to have strong carcinogenic and teratogenic effects on the human body, and has been banned from eating fish in most countries

Method used

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  • Application of curcumin to preparation of drug for killing fish epizoic infusorians
  • Application of curcumin to preparation of drug for killing fish epizoic infusorians
  • Application of curcumin to preparation of drug for killing fish epizoic infusorians

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: The killing effect of curcumin on the infective larvae of Melonella polyps

[0019] (1) Preparation of medicine: Weigh 2 mg of curcumin powder, use 20 μL of dimethyl sulfoxide (DMSO) to promote dissolution, add 761.25 μL of pure water, and make up a 2560 mg / L primary stock solution. Take 150μL of primary stock solution and 1350μL of pure water to make 1.5mL of 256mg / L secondary stock solution. The two-fold gradient dilution method was used to prepare 1.5 mL each of 128, 64, 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, and 0 mg / L (control group) liquids. Taking the DMSO content (0.13%) in the highest concentration chemical solution used in the experiment as the standard, a control solution containing 0.13% DMSO was prepared.

[0020] (2) Take a 96-well plate, add 100 μL of an aqueous solution containing about 500 larvae to each well, and then add 100 μL of the drug solution of different concentrations to each well, so that the final curcumin concentration in each well is...

Embodiment 2

[0023] Example 2: The killing effect of curcumin on the adult worm

[0024] (1) Drug preparation: the same as in Example 1.

[0025] (2) Add 200 μL of the insect liquid containing about 60 mature adults to each well of the 24-well plate and accurately count the number of adults in each hole, and then add 200 μL of curcumin liquid at different concentrations to make the final drug concentration sequentially 64, 32, 16, 8, 4, 2, 1, and 0 mg / L (control group), each drug concentration is set in 3 parallel. The experimental temperature was kept at 23±0.5℃, and the lethal time of each foraminifera within 5h and the number of surviving adults in each hole were recorded under a 4x objective lens, and the 5h mortality and half effective concentration (EC 50 ). After the above counting is completed, count the number of cysts formed in each well for 6 hours, and place the 24-well plate in a constant temperature incubator at 23±0.5°C, split and incubate for 16 hours, record the number of hatc...

Embodiment 3

[0029] Example 3: The killing effect of curcumin on the cysts of Melon

[0030] (1) Drug preparation: the same as in Example 1.

[0031] (2) Add 200μL of worm fluid containing about 30 mature cucurbita worm adults to each well of the 24-well plate, and let it stand for 6 hours. After the cysts are formed, count the number of cysts in each hole, and then add 200μL of different concentrations. The concentration of the final drug is 64, 32, 16, 8, 4, 2, 1, and 0 mg / L (control group), and three drug concentrations are set in parallel. After adding the medicine, place the 24-well plate in a constant temperature incubator at 23°C for 16 hours, observe the incubation under a 4x microscope, count the number of hatched larvae in each hole, and calculate the average number of hatched larvae per cyst, calculation formula as follows:

[0032] The average number of larvae hatched per cyst=the number of larvae in each hole / the number of cysts in each hole.

[0033] The results showed that: the hi...

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Abstract

The invention discloses application of curcumin to preparation of drug for killing fish epizoic infusorians, particularly relates to application to preparation of drug for killing ichthyophthirius multifilis, and belongs to the technical field of drug for killing parasites. According to the application, the curcumin is applied to the preparation of drug for fish epizoic infusorians for the first time, particularly for killing the ichthyophthirius multifilis; the parasites can be completely killed in the effective dosage range; and the effect of preventing and controlling ichthyophthiriasis is achieved. The application has the advantages that the curcumin sources are wide; the cost is low; the raw materials are rich; the preparation process is industrialized; no residue or no pollution on environment exists; no poison or no harm is caused on the human body; the half lethal concentration (56.8mg / L) on fishes is 187.4 times of the medium effective concentration (0.303mg / L) for killing the ichthyophthirius multifilis; safety is achieved on the fish; huge potential for developing novel fish drug for preventing and treating the fish epizoic infusorians is realized; and the the application prospects are wide.

Description

Technical field [0001] The invention belongs to the technical field of drugs for killing parasites, and relates to a new use of curcumin, in particular to the application of curcumin in preparing a drug for killing fish ectoparasitic ciliates. Background technique [0002] Parasitic ciliates disease is a common and frequently-occurring disease that harms fish in aquaculture. Multi-seed cucumis disease is a kind of parasitic ciliates disease, commonly known as "white spot disease". It is widely distributed worldwide, from Eurasia to the north to the Arctic Circle. The parasitic melon worm parasitizes in the epithelial tissues and gill filament tissues of various fishes, and strips the epithelial cells and red blood cells of the host, which can often cause thousands of fish species to die within a few days, causing serious economic problems. loss. For decades, chemical drugs have become the most effective way to prevent and control cucurbita vulgaris, but these chemical drugs hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P33/14
CPCA61K31/12
Inventor 张其中刘彦甍
Owner JINAN UNIVERSITY
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