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Application for morindone G and/or morindone O in preparation for medicine for killing fish parasites

A technology of Kuwanone and fish, which is applied in the field of fish parasite drugs, can solve the problems of high residue, environmental pollution, high toxicity, etc., and achieve the effect of simple extraction and preparation process, broad application prospects, and rich sources

Active Publication Date: 2014-07-30
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the hazards of high toxicity, high residue, carcinogenesis and teratogenicity, and environmental pollution, these chemicals have been banned in my country and even in the world.

Method used

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  • Application for morindone G and/or morindone O in preparation for medicine for killing fish parasites
  • Application for morindone G and/or morindone O in preparation for medicine for killing fish parasites
  • Application for morindone G and/or morindone O in preparation for medicine for killing fish parasites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: The killing effect of mulberry G, mulberry O, and a mixture of mulberry G and mulberry O (1:1) on the infective larvae of Melon

[0026] (1) Preparation of the drug: Weigh 5 mg of the manganone G, the manganone O monomer, and the mixture powder of the manganone G and the manganone O (mass ratio 1:1), use 20 μL of absolute ethanol to promote the dissolution, and add 3.105 mL of distilled water , Formulated into a 1600mg / L medicinal solution, diluted to the required concentration in the experiment by two-fold gradient dilution method. Taking the content (0.1%) of the absolute ethanol in the highest concentration chemical solution used as the standard as the standard, a control solution containing 0.1% absolute ethanol and no compound was prepared.

[0027] (2) Take a 96-well plate, add 100 μL of larva-containing liquid to each well, and then add 100 μL of different concentrations of medicinal liquid to each well. The medicinal liquids refer to mulberry G, mulberry O...

Embodiment 2

[0030] Example 2: The killing effect of mulberry G, mulberry O, and a mixture of mulberry G and mulberry O (1:1) on the adult (trophozoite) of the worm

[0031] (1) The drug preparation is the same as in Example 1.

[0032] (2) Take a 24-well plate, add 200μL of insect fluid containing about 60 mature trophozoites to each well and accurately count the number of adults in each hole, and then add 200μL of different concentrations of medicinal solution (2-fold gradient dilution) to make the final drug The concentration of the drug is 50, 25, 12.5, 6.25, 3.125, 1.78, and 0 mg / L (control), with 5 replicates of each drug concentration. The temperature of the experiment was kept at 23±0.5℃, and the total lethal time of each foraminifera and the number of surviving adults in each hole were observed and recorded under 4x objective lens, and the 4h lethality rate of mulberry G and mulberry O to the adult worms was calculated. And half effective concentration EC 50 (See Table 3).

[0033] Aft...

Embodiment 3

[0037] Example 3: The killing effect of mulberry G, mulberry O, and a mixture of mulberry G and mulberry O (1:1) on the cysts of the worm

[0038] (1) The drug preparation is the same as in Example 1.

[0039] (2) In a 24-well plate, inhale 200μL of the worm fluid containing about 60 mature cucurbita worm adults into each well, and let it stand for 6h. After the cysts are formed, count the number of cysts in each well, and then add 200μL of different The concentration of the drug solution is such that the final drug concentration is 50, 25, 12.5, 6.25, 3.125, 1.78, and 0 mg / L (control), with 5 replicates of each drug concentration. The mortality rate at 4h after encapsulation was added under a 4X microscope was recorded. After 4 hours, place the 24-well plate (undead cysts) in a constant temperature incubator at 23°C for 12 hours, observe the incubation under a 4x microscope, count the number of hatched larvae in each well, and calculate the average number of hatched cysts The nu...

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PUM

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Abstract

The invention belongs to the technical field of medicines for fish parasites, and discloses an application for morindone G and / or morindone O in preparation for a medicine for killing fish epizoic infusorians. According to the application disclosed by the invention, morindone G and morindone O are applied to preparation for a medicine for killing fish ectoparasites, and in particular for killing ichthyophthirius multifilis, for the first time, and all the parasites are killed in one time in an effective dosage range, so as to achieve the effects of preventing and treating ichthyophthiriasis; morindone G and morindone O are free from moraceae plants, are safe to people, are free from residues and pollution to environment, have median lethal concentrations which are 19 times and 13.4 times than that of the effective prevention and treatment concentration (2 mg / L) on fishes, and are safe to fishes in the effective dosage range for killing parasites; morindone G and morindone O are abundant in source, simple in extraction and preparation processes, and wide in application prospects.

Description

Technical field [0001] The invention belongs to the technical field of fish parasite medicines, and particularly relates to the application of mulberry G and / or mulberry O in preparing medicines for killing fish ectoparasitic ciliates. Background technique [0002] Parasitic ciliate disease is one of the most serious diseases that endanger aquaculture, especially cucurbitoccus. The disease is caused by the parasitic ciliates, which are parasitic on the gills and skins of fish, causing the fish to have difficulty breathing, and then a large number of diseased fish die, causing serious economic losses. For a long time, the prevention and treatment of melon worm disease has mainly been based on chemical drugs, such as malachite green and mercury preparations. However, due to their high toxicity, high residue, carcinogenic and teratogenic, environmental pollution and other hazards, these chemicals have been explicitly banned in my country and even internationally. Therefore, it is ...

Claims

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Application Information

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IPC IPC(8): A61K31/353A61K31/352A61P33/14
Inventor 张其中梁靖涵
Owner JINAN UNIVERSITY
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