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Application of Kuwanone g and/or Kuwanone O in the preparation of drugs for killing fish parasites

The technology of mulberry and fish is applied in the field of fish parasite medicine, can solve the problems of high residue, polluted environment, high toxicity and the like, and achieves the effects of abundant sources, simple extraction and preparation process, and broad application prospects.

Active Publication Date: 2015-11-04
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the hazards of high toxicity, high residue, carcinogenesis and teratogenicity, and environmental pollution, these chemicals have been banned in my country and even in the world.

Method used

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  • Application of Kuwanone g and/or Kuwanone O in the preparation of drugs for killing fish parasites
  • Application of Kuwanone g and/or Kuwanone O in the preparation of drugs for killing fish parasites
  • Application of Kuwanone g and/or Kuwanone O in the preparation of drugs for killing fish parasites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: The killing effect of Kuwanone G, Kuwanone O and the mixture of Kuwanone G and Kuwanone O (1:1) on the infective larvae of the multiseed melon worm

[0026] (1) Drug preparation: Weigh 5 mg of Kuwanone G, Kuwanone O monomer, and Kuwanone G and Kuwanone O (mass ratio 1:1) mixture powder respectively, dissolve with 20 μL of absolute ethanol, and add 3.105 mL of distilled water , formulated as a 1600mg / L drug solution, and then diluted to the concentration required for the experiment using the two-fold serial dilution method. Taking the content of absolute ethanol (0.1%) in the highest concentration drug solution used in the experiment as a standard, a compound-free control group treatment solution containing 0.1% absolute ethanol was prepared.

[0027] (2) Take a 96-well plate, add 100 μL of larva-containing solution to each well, and then add 100 μL of different concentrations of medicinal solutions to each well, and the medicinal solutions refer to Kuwanone G...

Embodiment 2

[0030] Embodiment 2: Kuwanone G, Kuwanone O and the mixture of Kuwanone G and Kuwanone O (1:1) are to the killing action of the adult (trophoblast) of the multispecies melon worm

[0031] (1) Drug preparation is the same as in Example 1.

[0032] (2) Take a 24-well plate, add 200 μL of worm liquid containing about 60 mature trophozoites to each well and accurately count the number of adults in each well, and then add 200 μL of different concentrations of liquid medicine (2-fold gradient dilution) to make the final medicine The concentrations of the drugs were 50, 25, 12.5, 6.25, 3.125, 1.78 and 0 mg / L (control) successively, and each drug concentration was repeated 5 times. The temperature of the experiment was maintained at 23±0.5°C, and the total lethal time of each foraminifera and the number of surviving adults in each hole were observed and recorded under a 4x objective lens, and the 4-h lethality rate of Kuwanone G and Kuwanone O on the adults of the multispecies melon w...

Embodiment 3

[0037] Example 3: Killing effect of Kuwanone G, Kuwanone O and the mixture of Kuwanone G and Kuwanone O (1:1) on polyzygotic melon cysts

[0038] (1) Drug preparation is the same as in Example 1.

[0039](2) In a 24-well plate, inhale 200 μL of liquid containing about 60 mature adults of the melon worm into each well, and let it stand for 6 hours. After the cysts are formed, count the number of cysts in each well, and then add 200 μL of different Concentration of the medicinal solution, so that the concentration of the final drug is successively 50, 25, 12.5, 6.25, 3.125, 1.78 and 0mg / L (contrast), each drug concentration is repeated 5 times. Record the death rate at the 4th hour after adding medicine to the cysts under a 4X microscope. After 4 hours, place the 24-well plate (undead cysts) in a constant temperature incubator at 23°C for 12 hours, observe the hatching situation under a 4X microscope, count the number of hatched larvae in each well, and calculate the average nu...

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Abstract

The invention belongs to the technical field of medicines for fish parasites, and discloses an application for morindone G and / or morindone O in preparation for a medicine for killing fish epizoic infusorians. According to the application disclosed by the invention, morindone G and morindone O are applied to preparation for a medicine for killing fish ectoparasites, and in particular for killing ichthyophthirius multifilis, for the first time, and all the parasites are killed in one time in an effective dosage range, so as to achieve the effects of preventing and treating ichthyophthiriasis; morindone G and morindone O are free from moraceae plants, are safe to people, are free from residues and pollution to environment, have median lethal concentrations which are 19 times and 13.4 times than that of the effective prevention and treatment concentration (2 mg / L) on fishes, and are safe to fishes in the effective dosage range for killing parasites; morindone G and morindone O are abundant in source, simple in extraction and preparation processes, and wide in application prospects.

Description

technical field [0001] The invention belongs to the technical field of fish parasite medicines, in particular to the application of Kuwanone G and / or Kuwanone O in the preparation of medicines for killing fish ectoparasitic ciliates. Background technique [0002] Parasitic ciliates are one of the most serious diseases that harm aquaculture, especially small melon worms. The disease is caused by the multi-spawn ciliates of the parasitic ciliates, which parasitize the gills and skin of fish in large numbers, causing difficulty in breathing, and then a large number of sick fish die, causing serious economic losses. For a long time, the prevention and treatment of small melon worm disease has mainly been based on chemical drugs, such as malachite green and mercury preparations. However, because these chemicals are highly toxic, highly residual, carcinogenic and teratogenic, and pollute the environment, they have been banned in my country and even in the world. Therefore, it is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/353A61K31/352A61P33/14
Inventor 张其中梁靖涵
Owner JINAN UNIVERSITY
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