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Application of rosemarinus officinalis extract in preparation of medicine for preventing and controlling ichthyophthirius multifiliis fouquet

A technology of rosemary extract and multi-seed melon worm, which is applied in the application field of rosemary extract in the preparation of medicines for preventing and controlling multi-seed melon worm, achieving the effect of broad application prospects and abundant resources

Inactive Publication Date: 2021-12-10
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of rosemary for the prevention and control of multiseed melon disease

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the killing action of rosemary extract to the infective larvae (predatory body) of small melon worm

[0021] (1) Drug preparation: Accurately weigh 0.1 g of rosemary extract, dissolve it with 1 mL of dimethyl sulfoxide, prepare a 100 g / L drug solution, and then dilute to the concentration required for the test. At the same time, a compound-free control solution containing 0.02% dimethyl sulfoxide was prepared.

[0022] (2) Set up 6 test groups, 1 dimethyl sulfoxide control group and 1 blank control group, set 3 parallels in each group, and complete the experiment in a 96-well plate. Add 160 μL of solution containing predators to each well of the 96-well plate, pipette 6 drops of 10 μL insect solution in each well and count on a slide to estimate the number of predators contained in 100 μL of the well. Add 100 μL of different concentrations of drug solution to each well, so that the final drug concentration is 8, 4, 2, 1, 0.5, 0.25 mg / L in sequence. Set a...

Embodiment 2

[0027] Embodiment 2: the killing action of rosemary extract to the adult (trophoblast) of small melon worm

[0028] (1) Drug preparation is the same as in Example 1.

[0029] (2) Set up 5 test groups, 1 dimethyl sulfoxide control group and 1 blank control group, set 3 parallels for each group, and complete the test in a 12-well plate. Add 1mL of insect liquid containing about 30 mature trophozoites to each well of the 12-well plate and accurately count the number of trophozoites in each well, and then add 1mL of different concentrations of liquid medicine respectively, so that the final drug concentration is 32, 16, 8, 4, 2 mg / L, the groups added with only distilled water and 0.05% dimethyl sulfoxide were set as negative controls. After incubating in a constant temperature incubator at 24°C for 6 hours, the number of surviving worms in each treatment group was recorded and the mortality rate was calculated. The 12-well plate was placed in a constant temperature incubator at ...

Embodiment 3

[0035] Embodiment 3: the extermination effect of rosemary extract to the cyst of the multi-seed melon worm

[0036] (1) Drug preparation is the same as in Example 1.

[0037] (2) Set up 5 test groups, 1 dimethyl sulfoxide control group and 1 blank control group, set 3 parallels for each group, and complete the test in a 12-well plate. Add 1mL of insect liquid containing about 30 mature trophozoites to each well of the 12-well plate and accurately count the number of trophozoites in each well, place the 12-well plate in a 24°C constant temperature incubator and incubate for 4 hours until the trophozoites develop into cysts Finally, accurately count the number of cysts in each well, and then add 1mL of different concentrations of the drug solution, so that the final concentration of the drug is 32, 16, 8, 4, 2 mg / L, and only distilled water and only 0.05% of the drug will be added. The dimethyl sulfoxide group was used as a negative control. Place the 12-well plate in a consta...

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Abstract

The invention discloses application of a rosemarinus officinalis extract in preparation of a medicine for preventing and controlling ichthyophthirius multifiliis fouquet, and belongs to the technical field of prevention and control of fishery diseases. According to the invention, the rosemarinus officinalis extract is applied to prevention and control of ichthyophthirius multifiliis fouquet for the first time, and 100% of killing rate of ichthyophthirius multifiliis fouquet can be achieved in an effective dose range. The median lethal concentration of the rosemarinus officinalis extract to goldfish respectively is 28.6 times and 9.4 times of the median effective concentration of the rosemarinus officinalis extract for killing ichthyophthirius multifiliis theront and trophozoite, and the rosemarinus officinalis extract is safe to fish within the effective range of killing insects. Rosemarinus officinalis resources are rich, the preparation process of the rosemarinus officinalis extract is simple, and the rosemarinus officinalis extract has the potential of being developed into a novel green alternative drug for preventing and controlling ichthyophthirius multifiliis fouquet.

Description

technical field [0001] The invention belongs to the technical field of fishery disease prevention and control, and in particular relates to the application of rosemary extracts in the preparation of medicines for preventing and controlling the plethysia melon worm. The invention also relates to a method for killing the plethysty melon worm. Background technique [0002] Ichthyophthirius multifiliis Fouquet is a parasitic disease caused by Ichthyophthirius multifiliis Fouquet (Ichthyophthirius multifiliis Fouquet) (abbreviated as Ichthyophthirius Fouquet) parasitic on the body surface and gills of freshwater fish. The rate is high, and in severe cases, it can cause a large number of fish to die and cause economic losses. At present, the prevention and control of multi-seeded melon worm disease in production mainly relies on chemical drugs such as formalin and copper sulfate, but the use of these drugs faces problems such as high residue, high toxicity, and unfriendly environm...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61P33/02A61K127/00
CPCA61K36/53A61P33/02A61K2236/333
Inventor 顾泽茂胡静波杨浩李丹
Owner HUAZHONG AGRI UNIV
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