66 results about "Gold fish" patented technology

The invention discloses an enzyme-linked immunosorbent kit for detecting the vitellogenin of goldfish, and a preparation method and applications thereof. The preparation method comprises the following steps: when in preparation, firstly, the pure product of the vitellogenin of the goldfish is prepared; secondly, the polyclonal antibody of rabbit anti-vitellogenin of the goldfish is prepared; thirdly, the polyclonal antibody of the rat anti-vitellogenin of the goldfish is prepared; and fourthly, respectively one bottle of the pure product of the vitellogenin of the goldfish, the polyclonal antibody of the rabbit anti-vitellogenin of the goldfish and the polyclonal antibody of the rat anti-vitellogenin of the goldfish, one block of blank 96-hole enzyme-linked immunosorbent assay board and respectively one bottle of the serum of a negative contrasting group, coating liquid, confining liquid, sample diluent, washing liquid, color development liquid, terminator and goat anti-mouse secondary antibody marked by horse radish peroxidase are filled into a box body together, thus obtaining the enzyme-linked immunosorbent kit for detecting the vitellogenin of the goldfish. The kit of the invention can be applied to the survey that the internal secretion disturbs chemical substances and can quantitatively detect the vitellogenin in the blood of the goldfish, liver tissues and the culture solution of hepatic cells, with sensitivity, accuracy and convenience.

The present invention relates to aquarium fish raising technology and is the raising method and special apparatus of raising aquarium fish. Unlike the background technology, which has aquarium fish capable of swimming only in aquarium, the present invention has some increased special apparatus for the aquarium fish to form certain fish stream with increased enjoying effect.

The invention relates to a test kit for qualitatively detecting fish vitellogenin by utilizing lipovitellin antibody. The test kit comprises pure product of goldfish lipovitellin and anti-rat goldfish lipovitellin polyclonal antibody. The preparation method of the test kit comprises the following steps: obtaining goldfish lipovitellin through purifying at first; then preparing anti-rat goldfish lipovitellin polyclonal antiserum through immune rats; and further purifying anti-rat goldfish lipovitellin polyclonal antibody. According to the invention, the test kit can be used for screening environmental estrogen materials and monitoring the situation of water environment which is polluted by the environmental estrogen materials, so that vitellogenin in fish blood, overall uniform serous fluid, skin mucus, liver tissues and hepatic cell nutrient solution can be monitored sensitively and conveniently. The test kit can be used for qualitatively detecting cyprinidae fish vitellogenin, and has the same monitoring sensitivity to the fish vitellogenin of a far provenance relationship fish. The biggest advantage of the test kit is that the test kit has a very wide application scope, and the minimum monitoring limit can reach 100ng / ml.

The invention relates to a method for detecting the insertion flanking sequence and copying quantity of a Tgf2 transposon in a goldfish genome. The method comprises the following steps of: extracting the DNA (Deoxyribose Nucleic Acid) of a goldfish genome with a Tgf2 transposon; performing restrictive incision enzyme digestion on the DNA of the genome; connecting an enzyme-digested DNA fragment with a splinkerette joint; and performing primary PCR (Polymerase Chain Reaction) and secondary PCR respectively to amplify flanking sequences of transposon insertion loci 5' and 3' respectively; and performing cloning and sequence measurement on a product of the secondary PCR to detect the flanking sequence of the transposon in a fish genome and presume an inserted copying quantity. The method has the advantages of easiness, convenience, high efficiency and low cost, and a rapid and accurate method can be provided for the detection of the copying quantities and flanking sequences of other fish genome insertion mutation and transgenic descendants mediated by the Tgf2 transposon.

The invention discloses a cultivation method for a hybridized goldfish. The cultivation method comprises the following steps: S1, taking bred red-eye white butterfly-type tail goldfishes as female parents and taking bred green long-tail ryukin goldfishes as male parents; hybridizing the two types of the goldfishes to obtain a first-filial generation F1; S2, selecting five-color butterfly-type tail ryukin goldfishes and ink-color butterfly-type tail ryukin goldfishes to be hybridized to obtain a second-filial generation F2; S3, selecting female and male three-color butterfly-type tail ryukin goldfishes from the second-filial generation F2, and carrying out self-hybridization on the female and male three-color butterfly-type tail ryukin goldfishes to obtain three-color butterfly-type tail ryukin goldfish breeds capable of stably inheriting three-color butterfly-type tail ryukin properties. According to the cultivation method, the selection and the hybridization of the goldfish breeds are carried out to cultivate the new goldfish breeds, so that the variety of the goldfishes is enriched.

The invention relates to the fields of cosmetic products and in particular relates to a golden hydrotherapy composition and a using method thereof. The golden hydrotherapy composition is prepared from the following components in parts by weight: 52-55 parts of wisdom golden water, 12-14 parts of gold introduction liquid, 6-7 parts of gold stock solution, 6-7 parts of thin skin growth promoting liquid, 6-7 parts of hyaluronic acid stock solution, 6-7 parts of black gold fish egg essence and 6-7 parts of snail protein stock solution. Compared with the prior art, the golden hydrotherapy composition has remarkable effects of fading and removing spots and is also capable of obviously increasing water content of skin and brightening whole skin color of a face.

The invention relates to a method for preparing a transparent skeleton specimen of a small animal, and a specimen prepared with the method. According to the invention, muscle of the small animal become transparent, and a colouring agent is adopted for soaking to develop and distinguish different parts of an animal body, so that original position structures of hard bones and cartilages of the animal body are showed directly and accurately. The method is simple in step, the adopted agent is safe and can be used for preparing transparent skeleton specimens of small animals such as goldfishes, tropical fishes, frogs and mice, the prepared specimens can be kept for a long time, structures are showed clearly, and the prepared specimens have relatively high scientific research and teaching values.

The invention relates to a fish molecular design breeding technology, in particular to a goldfish gene editing technology and a method for creating a new goldfish variety by using the gene editing technology. The method comprises steps of knockout target design, microinjection, fertilization incubation, gynogenesis and high-temperature sex reversal. According to the invention, a CRISPR / Cas9 gene editing technology is established on goldfish, so that a target phenotype is generated on F0 generation goldfish, an efficient gynogenesis condition of pleated Thai lion goldfish is determined through experiments, homozygotes are obtained in F1 generation, and a large number of groups are expanded in F2 generation through temperature-induced reversion. Through a molecular breeding means, transfer of a single target character is rapidly achieved, the goldfish gene editing technology and the method can be used for creating a new goldfish variety, provides technical support for molecular design and breeding of goldfish, and is beneficial to promote development of goldfish molecular breeding technology.

The invention relates to a method for detecting the insertion flanking sequence and copying quantity of a Tgf2 transposon in a goldfish genome. The method comprises the following steps of: extracting the DNA (Deoxyribose Nucleic Acid) of a goldfish genome with a Tgf2 transposon; performing restrictive incision enzyme digestion on the DNA of the genome; connecting an enzyme-digested DNA fragment with a splinkerette joint; and performing primary PCR (Polymerase Chain Reaction) and secondary PCR respectively to amplify flanking sequences of transposon insertion loci 5' and 3' respectively; and performing cloning and sequence measurement on a product of the secondary PCR to detect the flanking sequence of the transposon in a fish genome and presume an inserted copying quantity. The method has the advantages of easiness, convenience, high efficiency and low cost, and a rapid and accurate method can be provided for the detection of the copying quantities and flanking sequences of other fish genome insertion mutation and transgenic descendants mediated by the Tgf2 transposon.

The invention relates to a fish molecular design breeding technology, in particular to a method for creating jade rabbit pleated skirt Taishi goldfish. The method comprises the steps of target spot design, microinjection and fertilization incubation. On the basis of a CRISPR / Cas9 gene editing technology, after a pigment gene oca2 is knocked out, jade rabbit goldfish with red eye characteristics is generated on F0-generation goldfishes, efficient gynogenesis conditions and high-temperature group expansion conditions of pleated skirt Taishi goldfish are determined through experiments, and jade rabbit pleated skirt Taishi goldfish homozygotes are obtained on F1-generation goldfish; and through temperature-induced reversion, the F2 generation is massively expanded. By means of molecular breeding, transfer of the single-purpose character is rapidly achieved, the jade rabbit pleated skirt Taishi goldfish which is the variety with the high ornamental value is stably and rapidly created, and wide application prospects and market values are achieved.

Although an ornamental fish tank arranged in a modern home increases a lot of fun and color for family taste, the ornamental fish tank is high in manufacturing cost, and rare fishes such as goldfish and swallow fish are high in price and extremely difficult to manage and breed. For some rare endangered animals or dangerous animals, the close and free observation of the animals and no disturbance of the animals are problems to be solved. The invention discloses an electronic real scene viewing system which uses a high-definition camera or a camera to continuously shoot real scenes at multiple angles, the shot real scenes can be separated by one-way light-transmitting objects, a transparent glass room or a fish tank is formed by a plurality of screens through a receiving and displaying interaction unit in a viewing place, the synchronous and real scenes can be seen, and goldfish can be fed by pressing a button; a giant aquarium and a beast garden can be built in the venue, habits and food intake of dangerous organisms in a real scene can be viewed in a near-front manner, field interaction facilities can be controlled to interact, which is splendid, excited, sanitary and safe, and feelings similar to those in the field can be enjoyed.

The invention discloses a newly bought goldfish feeding water-passing intelligent motor device. The newly bought goldfish feeding water-passing intelligent motor device comprises a machine body, wherein a main space is formed in the machine body, a small space is formed on the lower side of the main space, a pause space is arranged on the lower side of the small space, and a pause device is arranged in the pause space. Newly bought goldfishes can automatically pass water, so that the goldfishes are prevented from directly entering a new environment of a fish tank and getting sick or dying due to the fact that the goldfishes do not adapt to the suddenly and violently changed environment. Two processes of temperature balance and water environment change balance can be controlled through a motor controlled through temperature. A motor controlled through continuous pressure is used for monitoring the possible inadaptation phenomenon of the goldfishes in the water passing process in real time, water passing is temporarily stopped to be buffered by the goldfishes, and it is guaranteed that water passing is normally completed.

The invention relates to the technical field of aquaculture, in particular to a household indoor goldfish culture method. The goldfish culture method comprises the following steps: injecting water into a fish tank, and illuminating and ventilating the fish tank to culture green algae and green moss in a water body; when green mosses appear on the tank wall of the fish tank or green algae appear in the water body, the original water in the fish tank is completely discharged, then new water is injected, the water is aerated, and then the goldfishes are put, and the putting density is that the putting number of the goldfishes with the length of 12-15 cm in each cubic meter of the water body is smaller than 18; the goldfish is fed with baits, the baits comprise granular baits and frozen blood worms, the granular baits and the frozen blood worms are fed at intervals, and after the granular baits are continuously fed for two days, the frozen blood worms are fed for one day; adjusting the water quality of the water body in the fish tank, namely extracting fish excrement at the bottom every day and supplementing the same amount of fresh water to the original water height.