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Method for building gyrodactylus purebred artificial infection system

A technology for establishing methods and three generations of worms, which can be used in biochemical equipment and methods, microbial determination/inspection, climate change adaptation, etc.

Inactive Publication Date: 2015-04-22
INST OF AQUATIC LIFE ACAD SINICA
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AI Technical Summary

Problems solved by technology

However, evaluation of the efficacy of fish medicines is difficult to accomplish due to the lack of an artificial infection system of pure monogenetic trematodes

Method used

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  • Method for building gyrodactylus purebred artificial infection system
  • Method for building gyrodactylus purebred artificial infection system

Examples

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Embodiment 1

[0034] Embodiment 1 The method for the establishment of the purebred artificial infection system of the third generation Kobayashi worm, the method is composed of the following steps:

[0035] 1) How to obtain healthy and insect-free goldfish: Temporarily raise goldfish purchased from the Flower, Bird, Fish and Insect Market in an aquarium filled with aerated tap water. After 1 week, soak them in 100mg / L formalin solution for 12 hours. Soak 3 times with an interval of 2 days. After the immersion, the goldfish were cultured in aerated tap water for 1 week. After randomly selecting 10 goldfishes and anesthetizing them with 50 mg / L MS-222 solution, observe the gills, body surface and fin rays of the fish under a stereomicroscope, and confirm that there are no third-generation worms before using them for the following infection experiments.

[0036] 2) Artificial infection method of third-generation worms: select a third-generation worm suspected to be third-generation worms from...

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Abstract

The invention belongs to the field of aquatic product disease prevention and treatment, and relates to a method for building a gyrodactylus purebred artificial infection system, in particular to a method for building an artificial infection system of gyrodactylus kobayashii hukuda purebred parasitized on the gill and the body surface of goldfish. The method includes the steps of (1) obtaining healthy and gyrodactylus-free goldfish; (2) conducting gyrodactylus artificial infections on the healthy and gyrodactylus-free goldfish; (3) maintaining the state after the gyrodactylus artificial infections are carried out. By means of the method for building the gyrodactylus purebred artificial infection system, an animal model is provided for screening medicine, developing new fish medicine and the like with the goldfish as hosts.

Description

technical field [0001] The invention belongs to the field of aquatic disease prevention and control, and relates to a method for establishing an artificial infection system of purebred three-generation insects, in particular to a method for establishing an artificial infection system for purebred third-generation Kobayashi parasites on goldfish. Background technique [0002] Gyrodactylus, known as the "fruit fly" in the parasite world, belongs to the flatworm phylum, monogenetic fluke class, third generation insect order, and third generation insect family. Gills, body surfaces and fin rays of seawater and freshwater fish. Since the discovery of the three-generation worm, the three-generation worm has attracted widespread attention because of its unique reproductive mode and strong host specificity, and it was used as a classic model for the study of embryonic development in developmental biology in the early days. Since the 1970s, foreign researches on three-generation wor...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12Q1/68
CPCA01K61/00C12Q1/6888Y02A40/81
Inventor 王桂堂周顺李文祥邹红杨宝娟张效平吴山功
Owner INST OF AQUATIC LIFE ACAD SINICA
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