134 results about "Immune adsorption" patented technology

The invention discloses a monoclonal antibody 64360-52D1 capable of specifically combining with SARS-Cov-2 virus structural nucleocapsid protein (N protein), a preparation method thereof, and 6 complementarity-determining regions (CDR) of heavy chain and light chain variable regions; and more specifically, the monoclonal antibody is secreted by a hybridoma strain 64360#52D1, and can specifically recognize the SARS-Cov-2 virus N protein rather than SARS virus N protein. Thus, the monoclonal antibody can be used for identifying the two coronaviruses with high similarity. The invention further provides an enzyme-linked immunosorbent assay (ELISA) and an immune colloidal gold test strip detection method for specifically detecting the SARS-Cov-2 virus N protein by preparing the 64360#52D1 antibody. The antigen of the antibody is SARS-Cov-2 virus N protein subjected to heat treatment and expressed in mammalian cells; the finally obtained antibody belongs to an IgG1 subtype; and a sequence for encoding the variable region of the antibody is obtained in a gene cloning mode.

The present application relates to antigen-binding proteins that are capable of binding to mammalian IgG. The frame-work regions of the antigen-binding proteins of the application preferably correspond to those of antibodies naturally that are devoid of light chains as may e.g. be found in camelids. The application further relates to nucleic acids that encode such antigen-binding proteins, to immunoadsorbent materials that comprise such proteins, to the uses of such immunoadsorbent materials for the purification of mammalian IgG antibodies and for therapeutic apheresis.

This invention discloses a citrinin immune chromatography test paper and its process method and utility. The test paper comprises pad, sample pad, tracing particle compound pad, pyroxylin film, adsorption pad, test thread, and control thread. The method is the following: to orderly put sample pad, tracing particle compound pad, pyroxylin film and adsorption pad on the pad; the tracing particle compound pad has citrinin antibody with tracing particle mark; the pyroxylin film has test thread and control thread; the test thread has citrinin antigen and antibody of citrinin antigen.

The invention discloses the production method and use for imidacloprid artificial hapten, artificial antigen and specific antibody, wherein the production method comprises, using imidacloprid (1-(6-chlorine-3-picolyl)-N-nitro-2-imidazoline imine) as raw material for reaction with 3-mercaptopropionic acid under alkaline condition, thus synthesizing hapten 1-(6-(2-carboxyethyl) sulfo-3-picolyl)-N-nitro-2-imidazoline imines (IM), then coupling with proteins through carbodiimide method and mixed anhydride method to prepare artificial antigens (immunogens and peridium antigens).

The invention discloses an enzyme-linked immunosorbent kit for detecting the vitellogenin of goldfish, and a preparation method and applications thereof. The preparation method comprises the following steps: when in preparation, firstly, the pure product of the vitellogenin of the goldfish is prepared; secondly, the polyclonal antibody of rabbit anti-vitellogenin of the goldfish is prepared; thirdly, the polyclonal antibody of the rat anti-vitellogenin of the goldfish is prepared; and fourthly, respectively one bottle of the pure product of the vitellogenin of the goldfish, the polyclonal antibody of the rabbit anti-vitellogenin of the goldfish and the polyclonal antibody of the rat anti-vitellogenin of the goldfish, one block of blank 96-hole enzyme-linked immunosorbent assay board and respectively one bottle of the serum of a negative contrasting group, coating liquid, confining liquid, sample diluent, washing liquid, color development liquid, terminator and goat anti-mouse secondary antibody marked by horse radish peroxidase are filled into a box body together, thus obtaining the enzyme-linked immunosorbent kit for detecting the vitellogenin of the goldfish. The kit of the invention can be applied to the survey that the internal secretion disturbs chemical substances and can quantitatively detect the vitellogenin in the blood of the goldfish, liver tissues and the culture solution of hepatic cells, with sensitivity, accuracy and convenience.

Indirect ELISA of deer's brucellosis belongs to the veterinary field and is used for diagnosing deer's brucellosis to improve the diagnosis level of the prior art. Biomaterials used in the assay of the invention comprise pig brucella vaccine 2 (S2) as an antigen, an enzyme labeled antibody rabbit anti-deer IgG-HRP, a standard positive serum, a standard negative serum and a serum to be assayed; and the assay comprises the following steps: coating the antigen, washing, adding the serum, adding the rabbit anti-deer IgG-HRP, adding a substrate liquid, terminating a reaction, testing by an enzyme-linked detector, adjusting to zero at 490nm, testing the OD value of each hole, and determining the result is positive when the OD value is equal to or greater than 0.32. According to the invention, the S2 is used as the antigen, the rabbit anti-deer IgG-HRP is used as the enzyme labeled antibody, and the indirect ELISA is used to diagnose the deer's brucellosis, so compared with previous diagnosis methods, the indirect ELISA of the invention has the advantages of good specificity, sensitivity and repeatability, and accurate diagnosis.

The invention provides a hybridoma cell strain 2C9, an anti-aflatoxin M1 monoclonal antibody produced by the secretion of the hybridoma cell strain 2C9 and application thereof. The hybridoma cell strain 2C9 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO.C201018. The hybridoma cell strain 2C9 can be used for preparing high-titer anti-aflatoxin M1 monoclonal antibody, and a method of rat ascites antibody ELISA (Enzyma-linked Immunosorbent Assay) is adopted to detect the titer which can reach 4.26*106. The ant-aflatoxin M1 monoclonal antibody has high sensitivity, the 50% of inhibition concentration IC50 aflatoxin M1 caused by the anti-aflatoxin M1 monoclonal antibody is 67pg / ml, and the cross reaction rates between the anti-aflatoxin M1 monoclonal antibody and aflatoxin B1, between the anti-aflatoxin M1 monoclonal antibody and aflatoxin B2, between the anti-aflatoxin M1 monoclonal antibody and aflatoxin G1 and between the anti-aflatoxin M1 monoclonal antibody and aflatoxin G2 are respectively less than 0.1%. The anti-aflatoxin M1 monoclonal antibody can be used for quick detection of aflatoxin M1.

The invention discloses a detecting method (ELISA) of enzyme link immune suction of banqi-enterobacteria and antibody and alpha-glucosidase and coded nucleic acid and carrier of the antibody, which is characterized by the following: using the amino acid sequence of alpha-glucosidase as Seq ID No:2; using ELISA of antibody to make agent box of the antibody; detecting the banqi-enterobacteria rapidly and effectively; providing high sensitivity, strong specificity and reliable result.

The invention discloses a process for preparing Quinclorac artificial semiantigen, artificial antigen, specific antibody and use thereof, wherein the preparation comprises, subjecting the dichloroquine (3,7-dichlorine-8-quinoline carboxylic acid) to sulfoxide chlorinated acylation, reacting with reanal and aminocaproic acid, thus obtaining semiantigen 4-(3,7-dichlorine-8-quinolineformyl) reanal or 6-(3,7-dichlorine-8-quinolineformyl) aminocaproic acid (QB or QC).

The invention relates to an enzyme couple immune detecting field, especially disclosing a clenobuterol enzyme immune agent box, relative detecting method, and a method for preparing the sample of animal organism, wherein said box comprises a box body, a 96 / 40 porous enzyme mark plate, a horse peroxide enzyme mark antibody, a clenobuterol standard solution, a substrate liquid, a substrate buffer liquid and a reaction ending liquid; the holes of said mark plate packs the packing antibody that combined with the clenobuterol antibody. The invention uses direct competitive enzyme couple immune adsorption analysis technique, to simplify the operation and reaction time, to reduce error. The inventive method has simple operation, low cost, and short time, while the extracted sample can be directly used in enzyme couple immune method and golden mark immune laminated analysis, to be sample of prior analysis.

The invention discloses a rabbit monoclonal antibody based ciprofloxacin residue analysis enzyme-linked immune adsorption kit. In the ciprofloxacin detection, the IC50 value of the kit is 1.41ng / mL, the linear fitting equation of an inhibition ratio curve is y = 0.0239x - 1.3159, the detection range is between 0.19 and 10ng / mL, and the lowest detection limit is 0.095ng / mL. The ciprofloxacin antibody in the kit has higher specificity, and has lower crossing-over rate with other quinolone micromolecules; and the cross reaction rates between the ciprofloxacin antibody and enrofloxacin, ofloxacin, norfloxacin, fleroxacin or pefloxacin are 28.8 percent, 13.1 percent, 11 percent, 22.6 percent and 20.4 percent respectively. The ciprofloxacin antibody has no cross reaction with other antibiotics and sulfanilamide medicaments. The kit is suitable for detecting ciprofloxacin residue in samples of milk, urine and the like. The kit can detect samples on a large scale at the same time, is convenient and quick, has quite important realistic significance for on-site supervision and trace analysis, meanwhile greatly reduces the detection cost, and has potential economic value.

The invention relates to method and agent box for detecting mammary gland globulin in the field of biology chemistry. The latex fixation leptospiral method comprises the steps of: a) mixing the mammary gland globulin antibody marked emulsion particle with the tested sample, b) quoting the test result by weather it has agglutination reaction, wherein the mammary gland globulin antibody marked emulsion particle is the emulsion particle which uses mammary gland globulin antibody R028 or R048 marked emulsion particle with the dilemma 2-10um; preserving the marked emulsion particle inside the HEPES buffer solution with pH 8.2. It also discloses a method for using the anti-mammary gland globulin monoclonal antibody and skin factor mark anti-mammary gland globulin monoclonal antibody to quantity test the mammary gland globulin by double clamp heart enzyme immune adsorption method. It also discloses a RT-PCR test mammary gland globulin expressing method and provides the agent box used to test the mammary gland globulin.

The invention discloses an artificial antigen of tetraodotoxin and a corresponding specific antibody and a preparation method and an application thereof. In the artificial antigen of the tetraodotoxin, the mass ratio of the tetraodotoxin to carrier protein is 1:10. In the invention, the artificial antigen is prepared from the tetraodotoxin which is coupled with carrier protein by a Mannich method, and the specific antibody is prepared from the artificial antigen immunization animals. The invention can be used for the extraction and purification of the artificial antigen aiming at the specific antibody of the artificial antigen of the tetraodotoxin, and can also be used for setting time-resolved immunofluorometric assay or an enzyme linked immunosorbent assay to detect the tetraodotoxin quickly and sensitively.

The invention discloses a method for preparing a complete antigen from a trimethoprim semiantigen compound T1 and a use of the complete antigen, and belongs to the technical field of biochemical engineering. An amino group at one end of a pyrimidine nucleus is derived to form active carboxyl, a trimethoprim carboxyl derivative (TMP-HS) with a single specific structure is obtained by purification, the derivative is the trimethoprim semiantigen compound T1, and the trimethoprim semiantigen compound T1 is coupled to a carrier protein to form the trimethoprim complete antigen T1-MA-BSA. The antigen or antibody can be used for building an enzyme-linked immunosorbent assay adsorption analysis method and a colloidal gold test paper rapid-detection method so that trimethoprim residue in foods can be fast detected.

The present invention concerns novel synthetic blocking reagents for the reduction of non-specific bindings in solid phase assays that rely on biological and specific recognition, e.g., in enzyme-linked immunosorbent assays (ELISAs). The invention provides the use of compounds as blocking reagents as well as kits comprising these compounds. The compounds comprise one or more poly(ethylene glycol) moieties, one or more alkyl- or aminoalkyl groups and another unit bridging the aforementioned groups, wherein the compound comprises at least one amino group.

The invention belongs to the technical field of food safety immunodetection and particularly discloses hapten, artificial antigen and antibody directly targeted to alternariol and a preparation method and application thereof. The alternariol hapten is of the structure as indicated in formula (I), wherein n=1, 2. The alternariol artificial antigen is of the structure as indicated in formula (III), wherein n=1, 2; carrier protein is keyhole limpet haemocyanin or bovine serum albumin or ovalbumin. The titer of antiserum obtained from an immune animal of the artificial antigen can reach 1: 128000, the minimum detection limit is 1.15 ng / mL, the half inhibiting concentration is 16.5 ng / mL, the antibody has the remarkable advantages of being high in specificity, good in sensitivity, high in accuracy and the like, therefore the antigen and the antibody can be used for establishing an alternariol enzyme linked immunosorbenption and analysis technology, and thereby the hapten, artificial antigen and antibody directly targeted to alternariol and the preparation method and application thereof can be used for rapidly detecting residual alternariol in food and have wide application prospect.

The invention discloses a chlorothalonil antigen, an antibody preparation method and a residual chlorothalonil ELISA (Enzyme-Linked Lmmuno Sorbent Assay) detection method. Chlorothalonil is used as an initial reactant; an amino group is used for replacing a 4-bite chlorine atom; an artificial antigen is prepared by using a 1'1-carbonyl diimidazole method; a rabbit-resistant chlorothalonil polyclonal antibody can be obtained by an immune New Zealand white rabbit; the antiserum titer can be determined to be 1:5.12*10<4> by using the ELISA; and on the basis, the residual chlorothalonil ELISA detection method can be established; and the lowest detection concentration by using the method is 0.383ng / mL.

The invention provides a H2S (hydrogen sulfide) releasing agent HA-ADT as well as a preparation method and an application thereof. The H2S releasing agent HA-ADT has the structural formula as followsin the description, wherein p is 4-6 and q is 16-22. By enzyme-linked immunosorbent assay, the concentration change of H2S in cell supernatant within 48 h and the concentration of H2S in the cell andthe supernatant in 24 h after cell administration are detected. Detection by Western blotting, MTT, EDU and Tunel methods shows that 200 mu mol / L of HA-ADT can promote cell apoptosis and inhibit cellgrowth when acting on human breast cancer cells MCF-7 and MDA-MB-231; detection by scratch, transwell method and invasion method shows that the HA-ADT can inhibit cell migration; tumor-forming experiments in nude mice show that the HA-ADT can inhibit growth of human breast cancer cells.

The invention relates to a combined medium and method for testing snail fever. It has the following characters: the medium contains recombination antigen mixture and recombination snail signal protein (rSj14-3-3) monoclonal antibody, and recombination antigen is mixed as recombination snail signal protein (rSj14-3-3) and recombination snail glutathione-s-transferase (rSjGST). The test operation is separately according to indirect enzyme immunosorbent test and double antibody bedded texture method. The invention adopts the two advantage diagnosis antigen molecule and its corresponding monoclonal antigen to form combined testing medium box.

The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

The invention provides preparation methods and application of pendimethalin hapten and antigen. The pendimethalin hapten is characterized by being prepared by taking N-(1-ethylpropyl)-3,4-dimethylaniline and 4-ethyl bromobutyrate to react to generate alkylated N-(1-ethylpropyl)-3,4-dimethylaniline, taking the alkylated N-(1-ethylpropyl)-3,4-dimethylaniline and concentrated nitric acid to react togenerate nitro-alkylated N-(1-ethylpropyl)-3,4-dimethylaniline, and then reacting with potassium hydroxide; the pendimethalin antigen is prepared by coupling the pendimethalin hapten and carrier protein. The antigen prepared by the invention has a specific pendimethalin antigen determinant, so that a high-specificity pendimethalin monoclonal antibody is possibly screened. The produced antibody hashigh specificity and high sensitivity and can be used for establishing an enzyme-linked immunosorbent assay method and a colloidal gold test strip determination method, so that rapid detection of pendimethalin in tobaccos and foods is realized.

The invention belongs to the field of biological separation engineering and relates to a protein A immune adsorption material used for purifying blood and a preparation method thereof. The invention discloses a biopolymer separating material coupled with agarose gel microspheres and recombinant protein A. An immune adsorption material with high protein A absorption performance is prepared according to the following steps: taking agarose gel as a carrier, activating with allyl glycidyl ether, coupling with sulfydryl on recombinant protein A amino terminal (C terminal) cysteine, and finally, blocking the terminal, thereby fixing the C terminal of the protein A on the agarose gel microspheres at a fixed point. The material has the advantages that the recombinant protein A on the agarose gel microspheres is uniform in structure and fixed in sequence, so that the adsorption capacity of the adsorption material for an antibody is promoted, and the adsorption material can be used for clinical immune adsorption treatment.

The invention relates to a method for fixing an antibody in a solid phase carrier in the immune adsorption reaction process. The method is characterized by comprising the following steps: enveloping FITC (fluorescein isothiocyanate) antibody on the solid phase carrier in advance, and then reacting 8-12 hours at 4-6 DEG C; sealing unreacted sites of the solid phase carrier, and then reacting 1-2 hours at 34-37 DEG C; adding an FITC mark antibody, then reacting 1-2 hours at 34-37 DEG C or 8-12 hours at 4-6 DEG C; and finally completing the enveloping of the solid phase carrier. According to the method provided by the invention, the problems that a current enveloped antibody is high in concentration, low in sensitivity, and poor in specificity are solved. The method that an enveloping layer is formed on the solid phase carrier in advance, and then the antibody is fixed has the advantages of simplicity, low cost, strong applicability, wide range and the like.

The invention relates to the technical field of detection, in particular to an aflatoxin-enzyme-linked immunosorbent assay device based on a nano-antibody and antigen analog peptide. An aflatoxin antigen mimic peptide uses immune prepared by an aflatoxin nano-antibody Nb28 as a target and is obtained through screening of a phage display technology, its amino acid sequence is shown as SEQ ID NO.3,SE QID NO.4, SEQ ID NO.5 or SEQ ID NO.6. For the first time, the nano-antibody Nb28 is applied to the antigen mimic peptide corresponding to the nano-antibody and meanwhile is used for aflatoxin-enzyme-linked immunosorbent assay (ELISA), and quick aflatoxin detection can be achieved. The genes and amino acid sequences of genetically engineered antibodies and antigen-mimicking peptides used in themethod can be determined and are easy to be produce, popularize and apply.

The invention relataes to novel evolutionary immunoglobulin binding molecules, as well as their preparation methods and applications. The invention discloses separated evolutionary immunoglobulin binding molecules, which are proteins with amino acid sequences as shown by SEQ ID NO : 1, or conservative variant proteins with immunoglobulin binding activity. The invention also discloses the gene encoding, genetic engineering preparation methods and applications of immunoglobulin binding molecules. The disclosed immunoglobulin molecule broad-spectrum combined with various immuneglobulin shows high immunoglobulin whole molecule binding activity, and can be used in large-scale purification of genetic engineering antibodies, purification of natural antibodies and monoclonal antibodies, enzyme-linked immunosorbent assay, and immuno-chromatography and immunohistochemical methods for immune antibody detection and diagnosis.

The invention relates to the technical field of blood purification materials and in particular discloses a high carrying capacity protein A immune adsorption material and a preparation method thereof.The protein A immune adsorption material is a high polymer material prepared from hydrophilic gel microspheres and a protein A through coupling; the hydrophilic gel microspheres are adopted as a carrier medium of the material, after being activated with epoxy chloropropane or oxidized with sodium periodate, the carrier medium reacts with ethidene diamine, and then an amino carrier is prepared; the obtained amino carrier reacts with bis-glycidyl ether to generate an activated carrier; and finally the activated carrier is coupled with the protein A. The material is high in content of the protein A in a coupled manner, high in adsorption efficiency on immune globulin and compounds thereof, and is applicable to clinical immune adsorption treatment.

The invention provides a micro-fluidic chip, which includes: a substrate; shaping electrode on a substrate; a gear shaping electrode on the substrate; an insulating layer covering the gear shaping electrode on the substrate; an electrode slice in the insulating layer and in electrical contact with the gear shaping electrode; a patterned bonding member with a hollow structure on the insulating layer; and a cover plate for sealing the hollow structure of the patterned bonding member therein, wherein the cover plate has an inlet hole and an outlet hole that are both communicated with the hollow structure. The invention also provides a manufacturing method of the micro-fluidic chip. The invention combines immune adsorption with the electrical characteristics of cells, a non-uniform electric field can be formed in a flow channel through the gear shaping electrode structure simply, cells receive a dielectrophoresis force, and non-targeted cells can break away without affecting targeted cell adsorption, thus maintaining high efficiency of cell immune adsorption while improving the adsorption purity under a flow rate conducive to specific adsorption.

An antigen capture IgA Enzyme Linked Immunosorbent Assay (ACA-ELISA) was developed for the detection of anti-flavivirus IgA. The assay utilizes flavivirus lysate antigen, preferably dengue virus lysate antigen captured by a monoclonal antibody. Captured anti-flavivirus IgA from test sera are preferably detected using rabbit anti-IgA conjugated with a reporter group such as horseradish peroxidase (HRP). The assay was found to be at least 8 times more sensitive than anti-human IgA capture ELISA (AAC-ELISA). The ACA-ELISA, based either on serum or saliva, was found to be more sensitive and rapid compared to the ''gold standard'' anti-dengue IgM detection technique and can be utilized as a diagnostic tool for the confirmation of dengue in the early phase of infection.

The invention relates to a protein A adsorption medium. The protein A adsorption medium is formed of a solid phase carrier material and a ligand fixed on the carrier by chemical coupling, wherein the ligand is recombinant protein A of an amino acid sequence represented by SEQ ID NO.1 in a sequence table. The protein A adsorption medium is tolerable to 20 minutes of thermal pressure sterilization at the temperature of 121.5 DEG C, so that a problem that a protein A immune adsorption column product is unable to perform final sterilization is resolved.

The invention discloses a preparation method and application of a carbendazim hapten and antigen. The method is characterized in that the carbendazim hapten is prepared from 2-aminobenzimidazole and butanedioic anhydride which are reacted; the carbendazim antigen is obtained by coupling the carbendazim hapten and carrier protein. The antigen prepared through the method presents a specific carbendazim antigenic determinantantigen epitope, so that screening out a high-specificity carbendazim monoclonal antibody becomes possible; the generated antibody is high in specificity and high in sensitivity, and can be used for building an enzyme-linked immunosorbent assay method and a colloidal gold test strip rapid determination method, so that carbendazim in tobacco and food is fast detected.