The invention discloses an
enzyme-linked immunosorbent kit for detecting the
vitellogenin of goldfish, and a preparation method and applications thereof. The preparation method comprises the following steps: when in preparation, firstly, the pure product of the
vitellogenin of the goldfish is prepared; secondly, the polyclonal
antibody of rabbit anti-
vitellogenin of the goldfish is prepared; thirdly, the polyclonal
antibody of the rat anti-vitellogenin of the goldfish is prepared; and fourthly, respectively one
bottle of the pure product of the vitellogenin of the goldfish, the polyclonal
antibody of the rabbit anti-vitellogenin of the goldfish and the polyclonal antibody of the rat anti-vitellogenin of the goldfish, one block of blank 96-hole
enzyme-linked immunosorbent
assay board and respectively one
bottle of the serum of a negative contrasting group,
coating liquid, confining liquid, sample
diluent, washing liquid, color development liquid, terminator and goat anti-mouse secondary antibody marked by
horse radish peroxidase are filled into a box body together, thus obtaining the
enzyme-linked immunosorbent kit for detecting the vitellogenin of the goldfish. The kit of the invention can be applied to the survey that the internal
secretion disturbs chemical substances and can quantitatively detect the vitellogenin in the blood of the goldfish, liver tissues and the culture solution of hepatic cells, with sensitivity, accuracy and convenience.