130 results about "Immune adsorption" patented technology

The present application relates to antigen-binding proteins that are capable of binding to mammalian IgG. The frame-work regions of the antigen-binding proteins of the application preferably correspond to those of antibodies naturally that are devoid of light chains as may e.g. be found in camelids. The application further relates to nucleic acids that encode such antigen-binding proteins, to immunoadsorbent materials that comprise such proteins, to the uses of such immunoadsorbent materials for the purification of mammalian IgG antibodies and for therapeutic apheresis.

The invention discloses an enzyme-linked immunosorbent kit for detecting the vitellogenin of goldfish, and a preparation method and applications thereof. The preparation method comprises the following steps: when in preparation, firstly, the pure product of the vitellogenin of the goldfish is prepared; secondly, the polyclonal antibody of rabbit anti-vitellogenin of the goldfish is prepared; thirdly, the polyclonal antibody of the rat anti-vitellogenin of the goldfish is prepared; and fourthly, respectively one bottle of the pure product of the vitellogenin of the goldfish, the polyclonal antibody of the rabbit anti-vitellogenin of the goldfish and the polyclonal antibody of the rat anti-vitellogenin of the goldfish, one block of blank 96-hole enzyme-linked immunosorbent assay board and respectively one bottle of the serum of a negative contrasting group, coating liquid, confining liquid, sample diluent, washing liquid, color development liquid, terminator and goat anti-mouse secondary antibody marked by horse radish peroxidase are filled into a box body together, thus obtaining the enzyme-linked immunosorbent kit for detecting the vitellogenin of the goldfish. The kit of the invention can be applied to the survey that the internal secretion disturbs chemical substances and can quantitatively detect the vitellogenin in the blood of the goldfish, liver tissues and the culture solution of hepatic cells, with sensitivity, accuracy and convenience.

The invention provides a hybridoma cell strain 2C9, an anti-aflatoxin M1 monoclonal antibody produced by the secretion of the hybridoma cell strain 2C9 and application thereof. The hybridoma cell strain 2C9 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO.C201018. The hybridoma cell strain 2C9 can be used for preparing high-titer anti-aflatoxin M1 monoclonal antibody, and a method of rat ascites antibody ELISA (Enzyma-linked Immunosorbent Assay) is adopted to detect the titer which can reach 4.26*106. The ant-aflatoxin M1 monoclonal antibody has high sensitivity, the 50% of inhibition concentration IC50 aflatoxin M1 caused by the anti-aflatoxin M1 monoclonal antibody is 67pg / ml, and the cross reaction rates between the anti-aflatoxin M1 monoclonal antibody and aflatoxin B1, between the anti-aflatoxin M1 monoclonal antibody and aflatoxin B2, between the anti-aflatoxin M1 monoclonal antibody and aflatoxin G1 and between the anti-aflatoxin M1 monoclonal antibody and aflatoxin G2 are respectively less than 0.1%. The anti-aflatoxin M1 monoclonal antibody can be used for quick detection of aflatoxin M1.

The invention relates to an enzyme couple immune detecting field, especially disclosing a clenobuterol enzyme immune agent box, relative detecting method, and a method for preparing the sample of animal organism, wherein said box comprises a box body, a 96 / 40 porous enzyme mark plate, a horse peroxide enzyme mark antibody, a clenobuterol standard solution, a substrate liquid, a substrate buffer liquid and a reaction ending liquid; the holes of said mark plate packs the packing antibody that combined with the clenobuterol antibody. The invention uses direct competitive enzyme couple immune adsorption analysis technique, to simplify the operation and reaction time, to reduce error. The inventive method has simple operation, low cost, and short time, while the extracted sample can be directly used in enzyme couple immune method and golden mark immune laminated analysis, to be sample of prior analysis.

The invention discloses an artificial antigen of tetraodotoxin and a corresponding specific antibody and a preparation method and an application thereof. In the artificial antigen of the tetraodotoxin, the mass ratio of the tetraodotoxin to carrier protein is 1:10. In the invention, the artificial antigen is prepared from the tetraodotoxin which is coupled with carrier protein by a Mannich method, and the specific antibody is prepared from the artificial antigen immunization animals. The invention can be used for the extraction and purification of the artificial antigen aiming at the specific antibody of the artificial antigen of the tetraodotoxin, and can also be used for setting time-resolved immunofluorometric assay or an enzyme linked immunosorbent assay to detect the tetraodotoxin quickly and sensitively.

The invention relates to the technical field of blood purification materials and in particular discloses a high carrying capacity protein A immune adsorption material and a preparation method thereof.The protein A immune adsorption material is a high polymer material prepared from hydrophilic gel microspheres and a protein A through coupling; the hydrophilic gel microspheres are adopted as a carrier medium of the material, after being activated with epoxy chloropropane or oxidized with sodium periodate, the carrier medium reacts with ethidene diamine, and then an amino carrier is prepared; the obtained amino carrier reacts with bis-glycidyl ether to generate an activated carrier; and finally the activated carrier is coupled with the protein A. The material is high in content of the protein A in a coupled manner, high in adsorption efficiency on immune globulin and compounds thereof, and is applicable to clinical immune adsorption treatment.

An antigen capture IgA Enzyme Linked Immunosorbent Assay (ACA-ELISA) was developed for the detection of anti-flavivirus IgA. The assay utilizes flavivirus lysate antigen, preferably dengue virus lysate antigen captured by a monoclonal antibody. Captured anti-flavivirus IgA from test sera are preferably detected using rabbit anti-IgA conjugated with a reporter group such as horseradish peroxidase (HRP). The assay was found to be at least 8 times more sensitive than anti-human IgA capture ELISA (AAC-ELISA). The ACA-ELISA, based either on serum or saliva, was found to be more sensitive and rapid compared to the ''gold standard'' anti-dengue IgM detection technique and can be utilized as a diagnostic tool for the confirmation of dengue in the early phase of infection.

The invention relates to a protein A adsorption medium. The protein A adsorption medium is formed of a solid phase carrier material and a ligand fixed on the carrier by chemical coupling, wherein the ligand is recombinant protein A of an amino acid sequence represented by SEQ ID NO.1 in a sequence table. The protein A adsorption medium is tolerable to 20 minutes of thermal pressure sterilization at the temperature of 121.5 DEG C, so that a problem that a protein A immune adsorption column product is unable to perform final sterilization is resolved.