The invention discloses a biocontrol strain YKY 01 for preventing and treating aromatic tobacco black shank and an anti-biological inoculant of the biocontrol strain YKY01, belonging to the technical field of bio-pesticides. The preservation number of the strain YKY01 is CGMCC (China general
Microbiological Culture Collection) NO.7723. The anti-biological inoculant is obtained by the following steps of
transplanting the strain YKY01 to a culture dish for activating, and culturing for 72 hours at 33 DEG C; transferring the activated
lawn into 300ml of NA culture solution which is contained in a 500ml triangular flask, culturing for 72 hours at the rotation speed of 175 revolutions / min under the temperature of 33 DEG C to obtain primary
fermentation liquor; inoculating the primary
fermentation liquor into the NA culture solution in a seed tank according to an
inoculation amount proportion of 1:150, thereby obtaining strain liquor for secondary
fermentation after fermenting for 48 hours with the temperature of the seed tank of 32 DEG C-35 DEG C; and inoculating the strain liquor for the secondary fermentation to a fermentation tank for fermentation according to the
inoculation amount proportion of 1:15, fermenting for 120 hours at the temperature of 32 DEG C-35 DEG C, and storing the strain liquor as biocontrol liquor, centrifuging for 3 minutes at 1500 rpm, and preparing with special-purpose
preservative fluid to form active
fungicide, so that the total concentration of viable
bacteria in the
fungicide is above1*1011 FU / ml. According to the biocontrol strain YKY 01 for preventing and treating aromatic tobacco black shank and the anti-biological inoculant of the biocontrol strain YKY01, the cost is low, the successive
cropping obstacle of the aromatic tobacco black shank can be effectively prevented, and the yield and the quality are improved.