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A kind of am mycorrhizal fungus mw21 and its biocontrol agent and application

A MW21, biocontrol fungicide technology, applied in applications, biocides, fungicides, etc., can solve the problems of not very significant and stable application improvement, and achieve the effect of no environmental pollution, protection of ecosystems, and reduction of large-scale use.

Active Publication Date: 2019-04-26
北京田源生物农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the improvement of these applications is not very significant, stable

Method used

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  • A kind of am mycorrhizal fungus mw21 and its biocontrol agent and application
  • A kind of am mycorrhizal fungus mw21 and its biocontrol agent and application
  • A kind of am mycorrhizal fungus mw21 and its biocontrol agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A biocontrol preparation AM mycorrhizal fungus MW21 powder, containing microbial components: AM mycorrhizal fungus MW21 (preservation number CGMCC No. 10058) is isolated from tobacco rhizosphere soil in Guizhou Province.

[0036] Isolation and identification method of AM mycorrhizal fungus MW21: separate AM fungal spores by wet sieve pour-sucrose centrifugation, weigh 100g soil sample in a centrifuge tube, add water to 2 / 3 of the centrifuge tube, centrifuge at 3000rpm for 3min, then remove the top Add 45%-50% sucrose solution to the supernatant, stir well and then centrifuge at 1500rpm for 1.5min quickly, pass through a 400-mesh sieve, and gently wash the spores and spores on the sieve with water, collect them in a petri dish with normal saline, and The number of spores was counted under a microscope and the morphology of the spores was observed.

[0037]Morphological characteristics of MW21: solitary in spore soil, spherical, white, 143‐330 (‐350) μm, occasionally oval...

Embodiment 2

[0038] Embodiment 2 MW21 powder process

[0039] The preparation method of the AM fungus MW21 powder is as follows:

[0040] 1) Evenly mix the soil containing the spores of the strain MW21 with the culture medium at a ratio of 1:80‐120 by volume. The matrix formula: peat soil (purchased from Dali Flower Sales Center, Qixia District, Nanjing) and steamed with hot air for at least 2 hours Coarse sand (purchased from Dali Flower Sales Center, Qixia District, Nanjing) mixed in 1:0.5‐3;

[0041] 2) Put the mixed spore-containing substrate into the sterilized ground pot (diameter 15cm, height 19cm), and plant sorghum, with 50-60 seeds in each pot, to ensure that the spores can fully contact the sorghum . After 4 months, the soil in the pot and the sorghum root system were taken out together, dried, pulverized to 800 mesh, and kept dry to prepare the soil containing mycorrhizal fungus spores for subsequent use;

[0042] 3) Preparation: mix the soil containing mycorrhizal fungal sp...

Embodiment 3

[0043] Example 3 Field Biocontrol Test of Woody Plant Diseases

[0044] Method: The healthy annual apple seedlings and grape seedlings were transplanted to the orchard (located in Taicang Modern Agricultural Park, Taicang, Jiangsu). When transplanting, 2g of AM fungus MW21 powder (prepared in Example 2) was evenly sprinkled on the roots, and each treated 48 seedlings, repeated 3 times. Then pour a sufficient amount of water to ensure the humidity, and use ordinary soil mixed with substrate as a control, and manage them uniformly according to conventional management methods. White-spotted feather disease and anthracnose are natural occurrences. After 4 months, the disease severity and control effects are counted. Symptoms of white-spotted feather disease: the diseased root is covered with a layer of white to off-white mycelium, and the cortex of the damaged root gradually turns brown and rots, and the outer cork is sheathed outside the xylem; when the root is seriously damaged...

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Abstract

The invention discloses an AM mycorrhizal fungus MW21 and its biocontrol agent and application. The main component of the arbuscular mycorrhizal fungus MW21 powder is Gigaspora albida MW21. Mix the soil containing the above mycorrhizal fungal spores with kaolin in a certain proportion, dry and crush, add 1% gum arabic, and add sodium dihydrogen phosphate to adjust the pH to 7.0, pass through a 300-mesh sieve, and dry to 3% water content, made into a powder with a concentration of 40-60 spores / g. Sprinkle the powder on the root circle of woody plants according to the dosage of 2g / plant, the control effect on woody plant diseases is more than 52.29%, the effect of promoting growth is more than 30.60%, and the survival rate of disease-free woody plants can be increased by 26.66% % or more, and improve the stress resistance of woody plants, and the growth-promoting effect reaches more than 10%.

Description

technical field [0001] The invention relates to the field of plant protection, and relates to an AM mycorrhizal fungus MW21 and its biocontrol agent and application. Background technique [0002] There are mainly 52 kinds of diseases of woody plants, including 32 kinds of leaf diseases, 9 kinds of branch diseases, 5 kinds of root diseases, and 6 kinds of flower and fruit diseases. The pathogens are fungi, bacteria, viruses, mycoplasma, nematodes, parasitic seed plants and non-infectious factors. Although some work has been done on disease prevention and treatment, the general attention is not enough, the light makes the growth of trees weak, reduces the ornamental value, and the serious ones die, causing irreparable social, ecological and economic benefit losses. For example, anthracnose, an aboveground disease, is a common and serious plant disease in agricultural production worldwide, causing serious economic losses to many trees, fruit trees, tea trees, ginkgo, etc., mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00A01P3/00A01P21/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 朱剑
Owner 北京田源生物农业科技有限公司
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