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Crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, preparation method and application thereof

A technology of Cryptococcus lorens and a biological preservative, which is applied in the field of crystal pear biological preservative and its preparation, and achieves the effects of increasing agricultural income and increasing efficiency, reducing application, and having outstanding biocontrol effects.

Active Publication Date: 2014-08-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a big gap in the control effect of antagonistic yeasts on pathogens compared with chemical fungicides. Therefore, how to use safe and effective methods to improve the antibacterial ability of antagonistic yeasts on postharvest crystal pears and other fruits is the key to current research. sexual problems

Method used

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  • Crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, preparation method and application thereof
  • Crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, preparation method and application thereof
  • Crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, preparation method and application thereof

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Embodiment Construction

[0035] The following is a detailed introduction to the method of using mixed bacteria to control the blue mold of crystal pear.

[0036] (1) The above-mentioned 12 strains of antagonistic bacillus are to the inhibitory effect of Penicillium expanses on the PDA plate:

[0037] 1.拮抗芽孢杆菌具有较好的体外抗青霉活性,分别为Bacillus subtilis AFB007、Bacillus subtilis AFB028、Bacillus cereus AFB037、Paenibacillus polymyxa AFB041、Bacillus subtilis AFB042、Bacillus pumilus AFB053、Paenibacillus polymyxa AFB074、Bacillus subtilis AFB096、Bacillus licheniformis AFB109, Bacllus cereus AFB114, Bacillus subtilis AFB124, Bacillus subtilis AFB138

[0038] 2. Beef extract-peptone medium: containing 10g of peptone, 5g of beef extract, 5g of sodium chloride, and 20g of agar, add water to the volume to 1000ml, adjust the pH to 7.0-7.2, and autoclave at 121°C for 20 minutes.

[0039] 3. Preparation of 12 kinds of antagonistic bacillus suspension

[0040] 1) Activation: 12 strains of antagonistic bacillus were cultured in...

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Abstract

The invention discloses a crystal pear biological antistaling agent based on mixed application of cryptococcus laurentii and antagonistic bacillus, a preparation method and application thereof. 12 strains of antagonistic bacilli are respectively employed for mixed application with the cryptococcus laurentii, they all can improve the postharvest biocontrol effect of crystal pears, and four strains can reach the best effect. Finally, the application concentration and ratio of the antagonistic bacillus and cryptococcus laurentii for a best mixed application effect are determined. Every 1ml of a control agent contains 106-109 antagonistic bacilli and 106-109 cryptococcus laurentii, and the best control effect can be ensured when the bacterial number ratio is 1:1. The cryptococcus laurentii and antagonistic bacillus involved in the biological antistaling agent both have the characteristics of safety and high efficiency, and have the advantages of environmental friendliness, and outstanding biocontrol effects, etc. the mixed bacteria antistaling agent provided by the invention can further reduce the application of chemical bactericides, and has important significance to food safety, environmental protection, agricultural income increase and synergy, and sustainable social development, etc.

Description

technical field [0001] The invention relates to the technical field of post-harvest disease prevention and control of fruits, in particular to a crystal pear biological preservative based on the mixed application of Cryptococcus laurentii and antagonistic bacillus and its preparation method and application. Background technique [0002] my country is one of the origins of the Pear family. Among the 25 species of the Pear family, there are 14 species in my country, accounting for more than half of the total. It is the country with the most varieties of pear trees in the world. As we all know, the damage caused by postharvest diseases of fruits and vegetables is huge, and postharvest diseases of fruits and vegetables are a global problem. According to reports, in developed countries, 10% to 30% of fresh fruits and vegetables are lost to post-harvest rot, while in developing countries, due to the lack of storage and transportation refrigeration equipment and backward transporta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/155C12N1/20C12N1/16C12R1/125C12R1/10C12R1/085C12R1/07C12R1/645C12R1/01
Inventor 周文文高慧沈洁郑晓冬热米拉李玮屠鹏程
Owner ZHEJIANG UNIV
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