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Biological control bacillus subtilis, production method, liquid fertilizer and application

A Bacillus subtilis and liquid technology, applied in the field of antagonistic bacteria, can solve problems such as pesticide residues in agricultural products, threats to human health, and environmental pesticide pollution, and achieve the effects of reducing the amount of chemical fertilizers, reducing pesticide residues and pollution, and reducing the amount of pesticides used

Active Publication Date: 2018-03-09
SHANGHAI LVLE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of chemical pesticides are used, one is to cause pesticide pollution to the environment, and the other is to bring about the problem of pesticide residues in agricultural products, which poses a threat to human health

Method used

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  • Biological control bacillus subtilis, production method, liquid fertilizer and application
  • Biological control bacillus subtilis, production method, liquid fertilizer and application
  • Biological control bacillus subtilis, production method, liquid fertilizer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0051] 1. Microbial liquid: the formula of the strain fermentation medium is 30g of soluble starch, 15g of soybean powder, 5g of sucrose, 5g of peptone, 3g of soybean meal, K 2 HPO 4 3g, MgSO 4 1g, bubble enemy 0.5g, add water to 1000ml. The effective number of viable bacteria in the microbial bacterial liquid is 9.8×10 9 individual / ml.

[0052] The fermentation conditions for the liquid fermentation production of Bacillus subtilis LVLE14 are: pH 6.5-7.5, culture temperature 20-40°C, initial rotation speed 100-200rpm, ventilation volume 0.5-1vvm, culture for 35-40 hours, during fermentation In the later stage, spores are formed, the spore rate is as high as more than 95%, and the number of effective viable bacteria in the fermentation broth is more than 10 billion / ml.

[0053] 2. Raw material formula: add according to the following formula. The trace element is borax and chelated zinc, the ratio is 2:3; the protective agent is sodium benzoate and molasses, the ratio is ...

Embodiment 1-2

[0061] 1. Preparation of solid microbial bacterial agent: ferment according to the method of Example 1, use peat to absorb the bacterial liquid after fermentation of biocontrol Bacillus subtilis LVLE14 according to the ratio of bacterial liquid: peat = 1:4, and compound the bacteria after mixing The effective number of live bacteria of the agent reaches 1.8×10 9 more than one per gram, and the water content should be kept within 30%.

[0062] 2. Sampling. The hydrolysis slag produced in the amino acid production process was obtained from Henan Lianhua Monosodium Glutamate Co., Ltd.;

[0063] 3. pH adjustment: crush large pieces of hydrolyzed slag into powder or small particles, mix with powdered lime according to hydrolyzed slag:lime=4:1, pulverize with chain crusher and stack for 8-12 hours to allow it to fully react;

[0064] 4. Turn over: use a forklift or manually turn over the pile, let the temperature of the reacted material cool down to normal temperature;

[0065] 5...

Embodiment 1-3

[0073] 1. Preparation of solid microbial bacterial agent: ferment according to the method of Example 1, use peat to absorb the bacterial liquid after fermentation of biocontrol Bacillus subtilis LVLE14 according to the ratio of bacterial liquid: peat = 1:5, and compound the bacteria after mixing The effective number of live bacteria of the agent reaches 1.2×10 9 more than one per gram, and the water content should be kept within 30%.

[0074] 2. Sampling. The oil residue produced by adsorption during the salad oil production process was obtained from Shanghai Lichang Environment Co., Ltd.;

[0075] 3. pH conditioning: crush large pieces of oil residue into powder or small particles, mix it with powdered lime according to oil residue:lime=4:1, crush it with a chain crusher and stack it for 8-12 hours to allow it to fully react;

[0076] 4. Turn over: use a forklift or manually turn over the pile, let the temperature of the reacted material cool down to normal temperature;

...

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Abstract

The invention provides a strain of bacillus subtilis with disease resistant activity. The bacillus subtilis with disease resistant activity is bacillus subtilis LVLE14, has the preservation date of June 20, 2016, and has the preservation number of CGMCC No.12639. The invention also provides a method for producing a liquid fertilizer by using the bacillus subtilis. The bacillus subtilis is used forfermentation production. The fermentation liquid is added into the liquid organic raw materials according to the mass ratio of 0.1 to 3 percent to obtain the liquid biological fertilizer; meanwhile,the production method of the biological organic fertilizer is provided. The invention also provides application of the bacillus subtilis to the effects of inhibiting the crop diseases and particularlyinhibiting powdery mildew of cucumber and gray mold of cucumber. The bacillus subtilis and the prepared fermentation liquid and fertilizer have the effective biological control effect; the preventionand treatment effect on the powdery mildew of cucumber and the gray mold of cucumber is better.

Description

technical field [0001] The invention relates to an antagonistic bacteria capable of preventing and eliminating various crop diseases—biocontrol bacillus subtilis and a production method thereof. Background technique [0002] At present, the prevention and control of diseases in our country basically adopts chemical pesticides, which are widely welcomed by farmers because of their strong toxicity, good control effect and low cost. my country produces more than 250 kinds of pesticides, more than 500 kinds of processed preparations, and more than 800,000 tons of raw materials, ranking second in the world. The annual consumption of pesticides is 800,000-1,000,000 tons, ranking first in the world. However, a large number of chemical pesticides are used, one is to cause pesticide pollution to the environment, and the other is to bring about the problem of pesticide residues in agricultural products, which poses a threat to human health. In order to reduce the use of chemical pes...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05F11/08C12R1/125
CPCC05B7/00C05F5/002C05F5/006C05F11/08C05G3/00C12N1/20C05G5/23C12N1/205C12R2001/125C05C5/02C05C9/00C05D9/00C05D9/02C05F7/00
Inventor 闫龙翔陈露林百全
Owner SHANGHAI LVLE BIO TECH
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