A functional strain, fermentation method, production method and application

A functional and bacterial strain technology, applied in the field of biocontrol Bacillus amyloliquefaciens and antagonistic bacteria, can solve the problems of agricultural product pesticide residues, human health threats, environmental pesticide pollution, etc., to reduce the amount of chemical fertilizers, reduce pesticide residues and pollution, The effect of reducing the use of pesticides

Active Publication Date: 2020-08-18
SHANGHAI LVLE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a large number of chemical pesticides are used, one is to cause pesticide pollution to the environment, and the other is to bring about the problem of pesticide residues in agricultural products, which poses a threat to human health

Method used

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  • A functional strain, fermentation method, production method and application
  • A functional strain, fermentation method, production method and application
  • A functional strain, fermentation method, production method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0049] 1. Microbial liquid: the formula of the strain fermentation medium is 25g of corn flour, 25g of bean cake powder, 1g of urea, 3g of sucrose, K 2 HPO 4 1.2g, MgSO 4 0.3g, MnSO 4 0.2, bubble enemy 0.3g, pH 7.2, add water to 1000ml. The effective number of viable bacteria in the microbial liquid is 1.49×10 10 individual / ml.

[0050] The fermentation conditions for liquid fermentation production of Bacillus amyloliquefaciens LVLE15 are: pH 6.5-8.0, culture temperature 30-45°C, initial rotation speed 120-200rpm, ventilation volume 0.5-1vvm, culture for 35-45 hours, middle and late stages of fermentation Spores are formed, the spore rate is as high as 93%, and the number of effective viable bacteria in the fermentation broth is above 15 billion / ml.

[0051] 2. Raw material formula: add according to the following formula. The trace element is borax and chelated zinc, the ratio is 1.5:3.5; the protective agent is sodium benzoate and molasses, the ratio is 1.5:2.

[0052]...

Embodiment 1-2

[0059] 1. Preparation of solid microbial agent: the fermented bacterial liquid of biocontrol Bacillus amyloliquefaciens LVLE15 is adsorbed with peat according to the ratio of bacterial liquid: peat = 1:5, and the effective number of viable bacteria of the compound bacterial agent after mixing reaches 1.2×10 9 more than one per gram, and the water content should be kept within 30%.

[0060] 2. Sampling. The hydrolysis slag produced in the amino acid production process was obtained from Henan Lianhua Monosodium Glutamate Co., Ltd.;

[0061] 3. pH adjustment: crush large pieces of hydrolyzed slag into powder or small particles, mix with powdered lime according to hydrolyzed slag:lime=4:1, pulverize with chain crusher and stack for 8-12 hours to allow it to fully react;

[0062] 4. Turn over: use a forklift or manually turn over the pile, let the temperature of the reacted material cool down to normal temperature;

[0063] 5. Pulverization: The hydrolyzed slag cooled to normal ...

Embodiment 1-3

[0071] 1. Preparation of solid microbial agent: the fermented bacterial liquid of biocontrol Bacillus amyloliquefaciens LVLE15 is adsorbed with peat according to the ratio of bacterial liquid: peat = 1:5, and the effective number of viable bacteria of the compound bacterial agent after mixing reaches 1.2×10 9 more than one per gram, and the water content should be kept within 30%.

[0072] 2. Sampling. The oil residue produced by adsorption during the salad oil production process was obtained from Shanghai Lichang Environment Co., Ltd.;

[0073] 3. pH conditioning: crush large pieces of oil residue into powder or small particles, mix it with powdered lime according to hydrolysis residue:lime=4:1, crush it with a chain crusher and stack it for 8-12 hours to allow it to fully react;

[0074] 4. Turn over: use a forklift or manually turn over the pile, let the temperature of the reacted material cool down to normal temperature;

[0075] 5. Pulverization: the oil residue cooled...

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Abstract

The present invention provides a functional bacterial strain, which is Bacillus amylolique faciens LVLE15, the preservation date is June 20, 2016, and the preservation number is CGMCC No.12640. The present invention also provides a method for understanding the production of liquid compound microbial fertilizer and solid compound microbial fertilizer by Bacillus amyloliquefaciens, and the fermented production by Bacillus amyloliquefaciens, the fermented liquid is added to liquid organic raw materials or processed solids at a mass ratio of 0.1-3%. in organic raw materials. Also provided is the application of the bacillus amyloliquefaciens in inhibiting crop diseases, especially inhibiting early blight of cucumber and gray mold of cucumber. The bacillus amyloliquefaciens and the fermented liquid and fertilizer prepared by the invention have effective biocontrol effects, and are particularly effective in preventing and controlling cucumber early blight and cucumber gray mold.

Description

technical field [0001] The invention relates to an antagonistic bacteria capable of preventing and eliminating various crop diseases—biocontrolling bacillus amyloliquefaciens, and to a production method of related fertilizers and the like. Background technique [0002] At present, the prevention and control of diseases in our country basically adopts chemical pesticides, which are widely welcomed by farmers because of their strong toxicity, good control effect and low cost. my country produces more than 250 kinds of pesticides, more than 500 kinds of processed preparations, and more than 800,000 tons of raw materials, ranking second in the world. The annual consumption of pesticides is 800,000-1,000,000 tons, ranking first in the world. However, a large number of chemical pesticides are used, one is to cause pesticide pollution to the environment, and the other is to bring about the problem of pesticide residues in agricultural products, which poses a threat to human health...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C05G3/00C05F11/08C05G3/60C12R1/07
CPCA01N63/00C05F5/002C05F5/006C05F11/08C05G3/00C12N1/205C12R2001/07C05F11/02
Inventor 闫龙翔陈露林百全
Owner SHANGHAI LVLE BIO TECH
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