40 results about "Blue mold" patented technology

The invention relates to a microbial agent used for producing a soil modifying agent by fermentation, consisting of fiber monad, bacillus, lactobacillus, methanobacteria, thiobacillus, streptomycete, high-temperature actinomyces, high-temperature monad, azotobacteria, nitration monad, nitration bacilus, rhizobium, koji mold, leaven, blue mold, detritus mold, rhizopus as well as mycorrhizal fungi and substrate. In addition, the invention also relates to a soil modifying agent produced by the fermentation of the microbial agent, application thereof and the like.

The present invention relates to a novel tobacco cultivar designated NC 2000, which is resistant to blue mold caused by the fungus Peronospora tabacina Adam. The invention provides seeds of the cultivar NC 2000, plants and parts thereof of the cultivar NC 2000, a tissue culture derived from the cultivar NC 2000, hybrids produced from cultivar NC 2000 and lines derived from cultivar NC 2000, as well as genetically modified forms of the foregoing plants and tissue culture. Also provided are methods of producing cultivar NC 2000 plants, cultivar NC 2000 hybrid plants, and tobacco lines derived from cultivar NC 2000.

The invention discloses Metschnikowia pulcherrima DPP2 with the collection number of CGMCC No. 8178. The Metschnikowia pulcherrima DPP2 has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, apple postharvest penicillium notatum and pear postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, pear postharvest blue mold and apple postharvest blue mold. In the selected plant pathogenic fungi, the inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are more than 20mm respectively, and the Metschnikowia pulcherrima DPP2 has the advantages of stability, high efficiency and broad-spectrum antibacterial property, and can be applied to control of pathogenic bacteria or diseases.

The invention discloses a biological prevention and control bacterial agent, and provides application of Metschnikowia zizyphicola sp. nov. XY201T to preventing and controlling plant diseases and application of the Metschnikowia zizyphicola sp. nov. XY201T to preparing a bacterial agent for preventing and controlling the plant diseases and refreshing harvested fruits and vegetables. The plant diseases comprise black spot, rot, gray mold, blue mold, soft rot, brown rot and green mold. The refreshing of the harvested fruits and vegetables is to inhibit the fruits and vegetables from rotting. Experiments prove that the separated Metschnikowia zizyphicola sp. nov. XY201T belongs to a new variety and has obvious effect of preventing and controlling the black spot of jujube, the rot of cucumber and eggplant and the like.

The natural fruit and vegetable preservative is prepared through mixing lilac flower extract and adsorbent, which is bentonite and active carbon. The lilac flower extract is prepared with lilac flower and through crushing, water soaking, distilling to obtain distillate, the first extraction of the distillate with solvent, the second extraction of the supernatant with sodium bicarbonate, the third extraction of the second supernatant with sodium bicarbonate, and volatilizing the solvent in the lower layer to leave the lilac flower extract. Mixing bentonite and active carbon first and mixing with the lilac flower extract then can produce the fumigating fruit and vegetable preservative. The fruit and vegetable preservative has obvious and lasting inhibition on blue mold and Aspergillus niger with serious infection to cut of fruit and vegetable, has active effective period as long as 15 months, and may be used in preserve various kinds of fruits and vegetable.

Strains of Metschnikowia species, in particular Metschnikowia pulcherrima, have been isolated from apple and shown to have antagonistic activity against Penicillium expansum, the causative agent of blue mold on apples. A unique feature of the strains is demonstrated activity at cold storage temperatures. The present invention is thus drawn to novel Metschnikowia isolates which are effective biological control agents against blue mold and to biocontrol compositions which include at least one of the isolates.

The invention provides an antagonistic strain 12a generating volatile bacteriostatic gas and application thereof in preventing and treating fruit-vegetable diseases. The antagonistic strain 12a is Bacillus vallismortis and is collected under CGMCC No.12179. The antagonistic strain 12a is obtained by collecting and separating peach tree rhizosphere soil from a Feicheng peach orchard without being applied with chemical fertilizer and pesticide, and the volatile bacteriostatic gas generated in fermentation liquid of the antagonistic strain 12a has strong antagonistic effect on Monilinia fructicola and also has obvious inhibiting effect on Marssonina mali caused by Alternaria mali, sweet potato black spot caused by Ceratocystis fimbriata Ellis.Et Halsted, citrus blue mold caused by Penicillium italicum Wehmer and winter jujube black spot caused by Alternaria alternata. The antagonistic strain 12a can effectively prevent and treat various fruit-vegetable diseases, is a biological preventing and treating potential strain which is good in biological preventing effect, pollution-free, wide in preventing and treating range, safe and ecological and has wide market application prospect.

The preparation process of biological yeast preparation for preventing and treating post-picking diseases of pear includes shaking table culture of Lorenzini cryptococcales in malt juice culture medium, centrifugal separation, collecting thallus, compounding Lorenzini cryptococcales suspension of 1E8-5E8 / ml or preparing dry powder with freeze drying machine at -40 to -45 deg.c, and storing. The biological yeast preparation of the present invention can tolerate harsh environment of high temperature, low temperature, field, etc. and may be used before or after picking of pear to prevent and treat blue mold and botrytis. It has no influence on the quality of pear and no harm on human body.

A broad-spectrum bactericide is prepared from the nano-porous material chosen from the molecular sieves of A, X and Y-type zeolite, mordenite, Si-Al zeolite, etc. and the silicate-base zinc oxide ions as active component. It is suitable for skin tinea fungus, multiple pathogenic funguses, and other 10 bacteria including candida, blue mold, aspergillus, etc.

The invention discloses Saccharomyces cerevisiae BY23, with broad bacteriostasis spectrum and stable effect, for controlling postharvest diseases of fruits and a use method and use thereof. The strainhas a collection strain serial number of CGMCC No. 14905 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the saccharomyces cerevisiae comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell / mL bacterial suspension from thalli and sterile water; placing the fruits such as apples, pears, grapes or oranges into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits, and drying the fruits in the air; and placing the fruits into a fresh-keeping box, and carrying out storage at normal temperature. The saccharomyces cerevisiae strain can be used for simultaneously controlling blue molds, Botrytis cinerea and black spot of apples, Botrytis cinerea of pears, black spot, Fusarium molds, anthrax and Phoma glomerata of grapes and blue molds of oranges, and loss caused by the postharvest diseases is reduced, sothat the saccharomyces cerevisiae strain has a very good application prospect.

The present invention discloses one kind of composite leaven for producing Chinese style fermented meat product. The composite leaven is prepared with dominant zymogen strains lactobacillus casei, fresh-colored staphylococcus and Nadi blue mold separated and screened from Qiang's meat product and Huniang 3.042 aspergillus oryzae for brewing soy sauce, and through domestication in meat, re-compounding, proliferating culture, centrifuging concentration, freeze drying, and mixing with protecting agent. The composite leaven for producing Chinese style fermented meat product has several microbe strains, high activity, fast fermentation, and capacity of producing meat product with traditional fermenting fragrance.

The invention discloses Saccharomyces ellipsoideus BY36, with broad bacteriostasis spectrum and stable effect, for preventing and treating postharvest diseases of fruits and vegetables and a use method and use thereof. The strain has a collection strain serial number of CGMCC No. 14910 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the Saccharomyces ellipsoideus comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell / mL bacterial suspension from thalli and sterile water; placing the fruits and vegetables such as apples, pears, grapes, strawberries, oranges or cherry tomatoes into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits and vegetables, and drying the fruits and vegetables in the air; and placing the fruits and vegetables into a fresh-keeping box, and carrying out storage at normal temperature. The Saccharomyces ellipsoideus strain can be used for simultaneously controlling blue molds and gray molds of apples, gray molds of pears, gray molds, aspergillosis, black spots, anthrax andpink rot of grapes, gray molds of strawberries, blue molds of oranges and gray molds and aspergillosis of cherry tomatoes, and loss caused by the postharvest diseases is reduced, so that the Saccharomyces ellipsoideus strain has a very good application prospect.

The invention belongs to the technical field of tobacco preparation, and particularly relates to a pectinase produced by blue mold-microzyme mixed fermentation and application thereof in a tobacco sheet working process. The pectinase is prepared by the steps of strain activation, seed solution preparation, fermentation culture, enzyme solution crude extraction and the like. The tobacco sheet and pectinase in a ratio of 1:(1-10) are subjected to enzymolysis in the crude enzyme solution at 40-50 DEG C for 2-6 hours, thereby treating the tobacco sheet. The overall technical line is as follows: the specific strains are subjected to mixed fermentation, the optimal fermentation conditions are screened and optimized to obtain the crude enzyme solution with the maximum pectinase enzyme activity, and the crude enzyme solution is used for treating the tobacco sheet to lower the pectinase content, thereby obtaining the optimal degradation effect. The maximum enzyme activity of the crude enzyme solution can reach 3880 U / mL. When the crude enzyme solution is used for treating the tobacco sheet, the pectinase content in the tobacco sheet can be lowered from 2.07% to 1.48%, thereby well improving the quality of the tobacco.

A blue cheese and a process for producing blue cheese from a liquid pre-cheese obtained by complete ultrafiltration of milk, and of which the blue mold develops only in the needling holes.

A straw-stalk feed with high bioactivity and nutritive value and low cost is prepared through preparing the culture medium from chicken droppings, straw and stalk, adding the composite enzyme for feed, adding useful bacteria chosen from trichoderma, blue mold and candida, and fermenting culture.

The invention discloses a separated streptomyce NBF715 and an application thereof in crop disease control. The separated streptomyce NBF715 is preserved in the China type culture collection center, and the preservation number is CCTCC NO: M2017414. It is firstly reported that the bacterial strain can be used for preventing and treating tobacco black shank, preventing and treating bacterial wilt oftomatoes, eggplants, peppers and gingers, preventing and treating citrus blue mold, preventing and treating tea target spot, and preventing and treating pepper anthracnose and potato late blight, andhas no cross resistance with a current bactericide and a pesticide. Compared with the current prevention and treatment of using a chemical synthesis pesticide, because the bacterial strain is from anatural environment, the bacterial strain has the advantages of high efficiency, low toxicity, environment-friendliness and difficult generation of resistance and the like, and has the extensive application prospect.

The invention discloses a pichia cactophila strain BY35 for effectively controlling fruit postharvest diseases with advantages of wide antibacterial spectrum and stable effect and a using method of pichia cactophila. The strain has a strain collection number of CGMCC No. 14909 in China General Microbiological Culture Collection Center. The using method of the pichia cactophila strain comprises thefollowing steps: activating the strain, performing fermentation culture by YPD, centrifuging, and preparing the culture into bacterium suspension of 1*10<8>cells per mL with sterile water; adding apples, pears, grapes or citrus and the like into the bacterium suspension, soaking for 30 seconds, taking out the fruits, and airing; and putting into a preservation box, and storing at a normal temperature. The pichia cactophila is capable of simultaneously controlling apple penicillium disease and botrytis cinerea, penicilliosis and botrytis cinerea of pear fruits and fusarium wilt disease and anthracnose of grapes as well as blue mold of citrus fruits. Losses caused by the postharvest diseases are decreased, and the strain has excellent application prospects.

The invention relates to the technical field of willow artware processing treatment, and discloses a moldproof and moistureproof treatment method for willows. The willows can be prevented from absorbing water, and wood color can be kept uniform; the treatment method has an extremely good killing effect on various types of aspergillus, trichoderma and blue molds, so that molds on the surfaces of the willows can be killed, and effective components can be diffused and permeate into willow tissues to prevent mildewing and worm damage inside; and treated willows have a long-term moldproof and moistureproof effects, and are efficient and environmentally-friendly novel products.

The invention discloses bactericidal agent compound which contains effective composition of propiconazole and can be applied in the prevention and cure of leaf spot which is hard to prevent and cure in agriculture and the application of the bactericidal agent compound. Composition A of propiconazole and composition B of imazalil are combined, and the mass ratio of the bactericidal agent compound is A:B=50-1:1-50. Auxiliary material is also included in the application and remixing of the compound, the composition A, the composition B and the auxiliary material are mixed to produce creaming agent, aqueous emulsion and suspending agent, two or more of the above are chosen as auxiliary material which is not limited within the above agents in the remixing. The compound is characterized in that by three times of applying the double bactericidal agent compound, the prevention and cure effect is at least 90 percent and at most 98 percent seven days after the last application of the compound. The compound is mainly applied for bananas and can also be applied for crops such as mandarin trees, wheat and the like, and has good prevention and cure effect for blue mold and green mold of mandarin trees, wheat sharp eyespot, root rot, powdery mildew and rust of wheat. The consumption of effect composition is decreased, costs are reduced, the occurrence of the drug resistance of bacteria is retarded, and environment pollution is decreased.

The invention discloses a strain BY25 of Pichia deserticola and a use method and applications thereof. The strain has a wide antibacterial spectrum, is used to control diseases of picked vegetables and fruits, and has a stable effect. The strain is preserved in China General Microbiological Culture Collection Center, CGMCC, and the preservation number is CGMCC No. 14906. The use method of Pichia deserticola comprises following steps: activating strains, using YPD to carry out fermentation culture, carrying out centrifugation, preparing bacterial suspension (1*10<8> cells / mL) from bacteria andsterile water; soaking fruits and vegetables (the apple, pear, grape, strawberry, orange, cherry tomato, etc.) in the bacterial suspension for 30 seconds, drying fruits and vegetables in the air, placing the fruits and vegetables in a fresh-keeping box, and storing the fruits and vegetables at a room temperature. The Pichia deserticola strain can control blue mold, gray mold, and black spot of theapple, blue mode and gray mold of the pear fruit, gray mold, aspergillosis, black spot, pink rot, and phoma disease of the grape, grey mold and aspergillosis of the straw berry, grey mold of orange,and grey mold and aspergillosis of the cherry tomato, reduces the loss caused by diseases after picking, and has a good application prospect.

The invention discloses a method for extracting and separating a flavonoid compound pinocembrin-7-O-belta-D-glucoside from a hairy fig and application of the pinocembrin-7-O-belta-D-glucoside and belongs to the technical field of botanical pesticides. Particularly the compound is separated with the method of subjecting a hairy fig crude extract to macroporous resin column chromatography, Sephadex LH-20 gel column chromatography and recrystallization, and the compound has good bacteriostatic activity to citrus postharvest pathogen-penicillium italicum and good disease resistance to citrus blue mold and can be used for developing a novel plant source citrus preservative.

A prothioconazole-containing sterilization composition comprises an active component A and an active component B with a weight ratio of 1:80-80:1, wherein the active component A is prothioconazole, and the active component B is one selected from thiophanate-methyl, carbendazim and prochloraz. The sterilization composition can be prepared into wettable powder, water-dispersible granules, a suspending agent, a suspoemulsion, an emulsion in water and a micron emulsion, is used to control diseases such as powdery mildew, sheath blight, fusarium wilt, leaf spot disease, rust disease, sclerotinia rot, virus disease, anthracnose, grey mold, ring rot, bakanae disease, rice blast, early blight, green mold, stem-end rot, blue mold, bipolaris oryzae, cladosporium cucumerinum, damping-off disease and the like, and has the characteristics of being small in pesticide usage amount, resistant to rain scrubbing and obvious in synergy.

The invention provides a plant disease combined fungicide. Especially the pesticide composition with prochloraz manganese salt and propineb as active ingredients, prochloraz manganese salt is prochloraz-manganese chloride, which is a broad-spectrum fungicide of imidazoles, which acts by inhibiting the biosynthesis of sterols. It has a good control effect on a variety of crop diseases caused by ascomycetes and semi-known fungi. Compared with prochloraz, the drug has high safety and is not easy to cause phytotoxicity. It is suitable for mushroom brown rot and brown spot. Mango anthracnose, citrus penicillium, green mold, anthracnose, pedicle rot, banana anthracnose, cucumber anthracnose, etc. Prosenb is a quick-acting, long-lasting, broad-spectrum protective fungicide, which is effective against It has a good protective effect against downy mildew of vegetables, tobacco, hops and other crops, as well as early and late blight of tomatoes and potatoes. It can control cabbage downy mildew, cucumber downy mildew, tomato morning and evening blight, mango anthracnose, and also has a certain inhibitory effect on powdery mildew, rust and diseases caused by Botrytis. It is safe for crops at the recommended dosage.

The invention provides green organic fertilizer for preventing diseases of grapes. The fertilizer is prepared from raw materials as follows: 25-35 parts of waste fungus chaff, 10-15 parts of cow dung,20-30 parts of peanut meal, 15-20 parts of peanut shells, 5-10 parts of a banana peel extraction solution, 4-10 parts of a leek root extraction solution, 5-9 parts of a purslane herb extraction solution, 0.6-1.4 parts of citric acid and 45-65 parts of water. Compared with the prior art, the fertilizer has the advantages that the fertilizer cost is low, waste is changed into wealth, besides, the fertilizer has comprehensive nutrients and can meet the growth requirement of grapes, so that planted grape leaves are greener, larger and richer, the grape health degree can be increased, and the disease resistance of the grapes can be improved. Besides, the fertilizer contains an antibacterial component and can effectively kill bacillus subtilis, staphylococcus aureus, aspergillus niger, mucor, blue mold and other bacteria and molds and prevent diseases of grapes.

The invention discloses a method for preventing blue mould pollution in koji made with soy sauce. The method comprises the following steps: after biological antagonistic yeast receives slant activation treatment, liquid extended cultivation is processed to the yeast; after being cultivated to be mature, the yeast accesses to koji material which has well stirred koji mold and is to be sent into a tank, with the addition of 10 to 10< 7> pieces of yeast / g koji material, and then enters into the koji tank to make koji. The method uses the antagonistic yeast to prevent the blue mould pollution in the process of koji making; during the koji making process, the adding of the antagonistic yeast does not bring negative effect to the koji material and the soy sauce after fermentation, and can improve the quality of the soy sauce.

The invention discloses Bacillus amyloliquefaciens KB with the collection number of CGMCC No. 8179. The Bacillus amyloliquefaciens KB has an inhibiting effect on peach postharvest monilinia fructicola, apple postharvest botrytis cinerea, tomato postharvest botrytis cinerea and apple postharvest penicillium notatum, as well as the inhibiting effect on peach postharvest brown rot, apple postharvest gray mold, tomato postharvest gray mold and apple postharvest blue mold. The inhibiting effect on the peach monilinia fructicola and the apple botrytis cinerea is most obvious, the diameters of inhibition zones are 22mm and 20mm respectively, and the inhibition rate against peach brown rot spots and apple gray mold spots achieves 100%.

A straw-stalk feed with high bioactivity and nutritive value and low cost is prepared through preparing the culture medium from chicken droppings, straw and stalk, adding the composite enzyme for feed, adding useful bacteria chosen from trichoderma, blue mold and candida, and fermenting culture.

The invention discloses a preparation GS115 / Ac-AMP2 for inhibiting pear-fruit postharvest penicilliosis. The preparation is recombinant yeast GS115 / Ac-AMP2 obtained after Ac-AMP2 antimicrobial peptideis introduced. A construction method for the recombinant yeast GS115 / Ac-AMP2 includes the following steps that optimized Ac-AMP2 genes are constructed onto pPICZalphaA vectors through Xho I and Not Idouble enzyme digestion and T4 ligase, the formed pPICZalphaA / Ac-AMP2 recombinant expression vectors are subjected to linear enzyme digestion, and then are electrically transformed into GS115 competent cells, a single colony PCR is selected in a Zeocin-contained resistant plate, sequencing verification is carried out, and a recombinant pichia yeast GS115 / Ac-AMP2 strain is obtained. The preparation GS115 / Ac-AMP2 can be used for refreshment for inhibiting fruit postharvest diseases.