Saccharomyces ellipsoideus for preventing and treating postharvest diseases of fruits and vegetables and preparation and use methods thereof

Abstract

The invention discloses Saccharomyces ellipsoideus BY36, with broad bacteriostasis spectrum and stable effect, for preventing and treating postharvest diseases of fruits and vegetables and a use method and use thereof. The strain has a collection strain serial number of CGMCC No. 14910 in General Microorganisms Center of China Committee for Culture Collection of Microorganisms. The use method of the Saccharomyces ellipsoideus comprises the steps: activating the strain, carrying out fermentation culture with YPD, carrying out centrifugation, and preparing a 1*10<8>cell / mL bacterial suspension from thalli and sterile water; placing the fruits and vegetables such as apples, pears, grapes, strawberries, oranges or cherry tomatoes into the bacterial suspension, carrying out soaking for 30 seconds, then, taking out the fruits and vegetables, and drying the fruits and vegetables in the air; and placing the fruits and vegetables into a fresh-keeping box, and carrying out storage at normal temperature. The Saccharomyces ellipsoideus strain can be used for simultaneously controlling blue molds and gray molds of apples, gray molds of pears, gray molds, aspergillosis, black spots, anthrax andpink rot of grapes, gray molds of strawberries, blue molds of oranges and gray molds and aspergillosis of cherry tomatoes, and loss caused by the postharvest diseases is reduced, so that the Saccharomyces ellipsoideus strain has a very good application prospect.

Description

technical field [0001] The invention relates to the field of biological control of post-harvest diseases of fruits and vegetables, in particular to a wine yeast (Saccharomyces ellipsoideus) used for the biological control of post-harvest diseases of fruits and vegetables. The main postharvest diseases have significant control effects. Background technique [0002] Although the quality deterioration of fresh fruits and vegetables is affected by many factors, disease is the main reason. Among them, rot and deterioration caused by fungal diseases is the most serious factor in postharvest fruit loss. Although postharvest diseases of fruits and vegetables can be prevented and controlled in many ways such as agricultural control, physical control, chemical control and biological control, the main measure at present is chemical control (Eckert & Ogawa, 1985, 1988). However, long-term use of chemical pesticides not only leads to the development of drug resistance of pathogenic bac...

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Problems solved by technology

[0004] However, although there are nearly a hundred species of antagonistic yeasts reported at home and abroad, the biocontrol effects of most antagonistic yeasts have only been verified on a few fruits.

However, since the biocontrol effects of different strains of the same yeast are very different (Filonow et al., 1996), most of the antagonistic yeasts lack strains with broad antibacterial spectrum and stable effect.

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