Separated streptomyce NBF715 and application thereof in crop disease control

A technology of Streptomyces and Streptomyces brownus, applied in the direction of application, bacteria, microorganisms, etc., can solve problems such as environmental pollution, pesticide residues, and drug resistance of bacteria, and achieve the effect of not polluting the environment and not easily producing drug resistance

Active Publication Date: 2018-08-17
WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Since the 1950s, pesticides have played an important role in crop production. However, the wide application of chemical pesticides h

Method used

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  • Separated streptomyce NBF715 and application thereof in crop disease control
  • Separated streptomyce NBF715 and application thereof in crop disease control
  • Separated streptomyce NBF715 and application thereof in crop disease control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Obtaining and Identification of Streptomyces enissocaesilis NBF715

[0036] An isolated strain of Streptomyces was isolated from the soil of Hefeng County, Enshi, and was identified as Streptomyces enissocaesilis after 16s rDNA combined with physiological and biochemical identification ( figure 1 , Table 1, Table 2), has been sent to the China Center for Type Culture Collection on July 7, 2017 for preservation, classification and name: Streptomycesenissocaesilis (Streptomycesenissocaesilis) NBF715; preservation number is CCTCC NO: M2017414; location: China, Wuhan, Wuhan University.

[0037] In the present invention, Streptomyces enissocaesilis NBF715 may be referred to as NBF715 for short.

[0038] Morphological characteristics: The colony is round, with smooth edges and dense surface. The aerial hyphae are silver-gray, the base mycelium cork is yellow, and the colony is round, without pigment. The spore filaments are distributed in a straight chain, and the spores a...

Embodiment 2

[0049] Fermentation of Streptomyces enissocaesilis NBF715:

[0050]Preparation of Streptomyces enissocaesilis NBF715 seed solution: including activating the slant-preserved bacterial species on the ISP-2 plate, picking and inoculating it into 100 ml of seed medium, at 28°C, on a shaker at 160 rpm Cultivate for 4 days; the seed solution is obtained.

[0051] The formula of the seed medium is: 1L of medium includes 20g of mannitol, 10g of soybean peptone, 0.35g of potassium dihydrogen phosphate, 0.5g of calcium carbonate, 20 μL of soybean oil, and the rest is water.

[0052] Solid fermentation: Inoculate Streptomyces enissocaesilis (Streptomyces enissocaesilis) NBF715 seed liquid into solid medium at 10%, with a sample loading of 20%, and culture at 30°C for 10 days. The bacterial concentration of the culture obtained by this method is 5*10 10 spores / gram.

[0053] The solid fermentation medium is: 83% bran, 3% glucose, 5% potassium nitrate, 3% dipotassium hydrogen phosphate,...

Embodiment 3

[0057] Streptomyces enissocaesilis NBF715 prevents tobacco black shank:

[0058] (1) Antibacterial activity of NBF715 strain against Phytophthora nicotianae in vitro

[0059] The pathogenic bacteria of tobacco black shank tested were isolated from tobacco black shank plants, and stored on V8 slope at 10°C for later use. The slant strain was transferred to the V8 plate for activation, and cultured at 30°C for 6 days for later use. NBF715 was activated from the slant to ISP2 medium for 7 days for later use. Use a 4mm hole puncher to punch out Phytophthora nicotianae bacteria discs and place them in the center of the PDA medium, then use a 4mm hole puncher to punch out NBF715 bacteria discs and place them on both sides of the Phytophthora nicotiana plate with a distance of 25mm. After 8 days, the inhibition zone was observed. Obvious bacteriostatic zone (diameter of bacteriostatic zone is 2cm) can be observed through in vitro confrontation, indicating that NBF715 strain has a ...

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Abstract

The invention discloses a separated streptomyce NBF715 and an application thereof in crop disease control. The separated streptomyce NBF715 is preserved in the China type culture collection center, and the preservation number is CCTCC NO: M2017414. It is firstly reported that the bacterial strain can be used for preventing and treating tobacco black shank, preventing and treating bacterial wilt oftomatoes, eggplants, peppers and gingers, preventing and treating citrus blue mold, preventing and treating tea target spot, and preventing and treating pepper anthracnose and potato late blight, andhas no cross resistance with a current bactericide and a pesticide. Compared with the current prevention and treatment of using a chemical synthesis pesticide, because the bacterial strain is from anatural environment, the bacterial strain has the advantages of high efficiency, low toxicity, environment-friendliness and difficult generation of resistance and the like, and has the extensive application prospect.

Description

technical field [0001] The invention belongs to the technical field of crop prevention and control, and in particular relates to an isolated streptomyces NBF715 and its application in crop disease prevention and control. Background technique [0002] Since the 1950s, pesticides have played an important role in crop production. However, the wide application of chemical pesticides has caused problems such as environmental pollution, pesticide residues, destruction of ecological balance, and drug resistance of pathogens. Based on the above reasons, more and more people pay attention to biological pesticides, which have the advantages of no pesticide residues, environmental friendliness and resistance to pesticides. Streptomyces (Streptomyces) is a higher actinomycetes, which can produce a variety of antibiotics, plant hormones and other active substances, which play an important role in improving the disease resistance and stress resistance of plants, and have the potential to ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P1/00A01P3/00C12R1/465
CPCA01N63/00C12N1/20C12N1/205C12R2001/465
Inventor 黄大野万中义曹春霞张亚妮刘晓艳王开梅姚经武朱志刚
Owner WUHAN CHUQIANG BIOLOGICAL TECH CO LTD
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