66results about How to "Infectious" patented technology

The invention discloses a Seneca valley virus strain and an application thereof, wherein the Seneca valley virus SVV-HeNXX/swine/2017 is deposited in China Center For Type Culture Collection with theserial number of CCTCCNO: V201767. The Seneca valley virus SVV-HeNXX/swine/2017 has good immunogenicity. It can induce immune animals to produce higher level of immune protection and can be used for preparing vaccine against Seneca Valley Virus Disease. The vaccine has high safety and no detoxification after immunization, and can induce animals to produce higher level of antibody quickly, so as toachieve high-efficient prevention of Porcine Seneca Valley Virus Disease, and has good popularization and application value.

The present invention discloses recombinant vaccine based on the Helicobacter pylori neutral granulocyte activated protein A (NapA) antigen and its preparation process and application in inducing the protecting immune reaction resisting Helicobacter pylori infection. The vaccine consists of independent Helicobacter pylori NapA as the basic active component, or the fusion protein comprising Helicobacter pylori NapA, adhesion HpaA and urase B subunit active segment (UreB414), and one or several kinds of pharmaceutically acceptable adjuvant and excipient.

The invention discloses Acremonium hansfordii Ahy1 and Acremonium hansfordii wettable powder. The Acremonium hansfordii wettable powder comprises the following components in percentage by weight: 10 percent of Acremonium hansfordii conidium powder, 2 percent of wetting agent, 5 percent of dispersing agent, 0.8 percent of ultraviolet protective agent, 1 percent of stabilizing agent and the balance of carrier. The Acremonium hansfordii Ahy1 is screened and separated in a drought environment, is different from strains from other areas due to the geographic environment in which the Acremonium hansfordii Ahy1 is positioned, and has good property of high stress resistance. Meanwhile, indoor and field efficacy proves that the Acremonium hansfordii wettable powder has high infectivity on aphid.

The invention discloses a method for separating halimasch from endophytic fungi of gastrodia elata, thereby solving problems that the existing halimasch separation method is limited in separation material, prone to infectious microbe infection, prone to strain degradation and the like. The invention relates to a method for separating halimasch from endophytic fungi of gastrodia elata creatively; the method comprises the steps of pretreatment of gastrodia elata, tissue separation, tissue culture, separation of endophytic fungi, purification and preservation. The halimasch strain subjected to separation, purification and subculture need to be subjected to cultivation tests with gastrodia elata before application to production; the cultivation tests include asexual cultivation of hemp and halimasch and sexual cultivation of gastrodia elata seeds, germinating bacteria and halimasch. The method is high in practicability and convenient to operate; with the method, effects that the separationpollution rate is low, and the separated halimasch strains are high in infectivity and not prone to degradation in gastrodia elata cultivation can be realized.

The The invention relate to the art reflective watercolor and the preparation method, belonging to technical field of painting things, which is characterized in that first the gum Arabic is put into distilled water and dissolved completely after swelling, heating and blending. The water soluble starch, the glycerin, the sodium benzoate and the sodium hexametaphosphate are mixed and the then the bond can be made by blending evenly. The powdered pigment is added into the bond and blended evenly. The aluminum silicate and the hydrated silicon dioxide are added in and then blended evenly to make rough sizing agent. The rough sizing agent is grinded in the hopper of a three-roller muller to make color sizing agent. The product can be obtained by blending in a vacuum mixer after adding the ethanol and glass microballoon in the color sizing agent. The whole image can show splendent result when lighting in dark environment after using the art reflective watercolor, which can show the infectivity and the attraction of the artwork better.

The invention discloses a water curtain pavilion with a water screen movie. The water curtain pavilion comprises a water curtain pavilion body, a human infrared sensor is arranged on the periphery of the water curtain pavilion body and is connected with the input end of a controller module, an amplifying circuit, a filtering circuit and an A/D conversion circuit which are sequentially connected are connected between the controller module and the human infrared sensor, the human infrared sensor is connected with the amplifying circuit, the output end of the controller module is connected with projection equipment, sound equipment and lighting equipment, the controller module is further connected with a data storage circuit module and a storage battery, and the storage battery is connected with the solar battery panel through a charging circuit module. The water curtain pavilion is simple in structure, novel and reasonable in design, vivid and picturesque in vision effects, full of appeal, energy saving, environment friendly and highly practical.

The invention relates to an application of a mouse leukemia cell L1210 in the preparation of an HIV-1 infectible host cell, and also relates to the HIV-1 infectible host cell. The mouse leukemia cell L1210 is a mouse leukemia cell L1210 with CD4 and CCR5 expressed on the surface of the cell and Cyclin T1 expressed in the cell. The invention also relates to a preparation method of the HIV-1 infectible host cell, and an application of the HIV-1 infectible host cell. The HIV-1 infectible host cell obtained in the invention can be highly-efficiently infected by HIV-1, virus particles replicated in the infected cell has infection activity, and the infected cell secretes active HIV-1 virus to the outside of the cell. The HIV-1 infectible host cell can be used for treating and researching HIV-1 infection and developing anti-HIV-1 drugs.

The invention provides a rapid virus detection method based on a single-molecule total internal reflection fluorescence imaging technology. The method comprises the following steps: step A, adding a fluorescent nucleic acid probe into a virus standard solution as a to-be-detected solution; step B, putting the to-be-detected solution on a total internal reflection fluorescence imaging device for fluorescence signal detection, calculating a diffusion coefficient according to the dynamic change of a tracked fluorescence signal phase point, taking the diffusion coefficient as a single molecular dynamics fingerprint signal of virus particles, and calculating the average hydrodynamic diameter of the virus by utilizing a Stocks-Einstein equation; and step C, replacing the virus standard substancewith a to-be-detected virus sample, obtaining a monomolecular kinetic fingerprint signal and a hydrodynamic diameter of the to-be-detected virus according to the same operation, and indicating that the to-be-detected virus sample contains the same type of virus if the monomolecular kinetic fingerprint signal and the hydrodynamic diameter conform to the monomolecular kinetic fingerprint signal andthe average hydrodynamic diameter. Quantitative detection can be realized by counting the two-dimensional paths of the fluorescence point signals conforming to the diffusion coefficient. The method has the advantages of simplicity, rapidness, high efficiency and economy, and is suitable for large-scale rapid detection of viruses.

The invention discloses a construction method and application of an antheraea pernyi multinucleocapsid nucleopolyhedrovirus shuttle vector, and belongs to the field of a biological technology. A bacterium artificial chromosome cloning vector is used for constructing a transfer vector comprising a DNA fragment containing a lacZ alpha report gene and a bacterial transposon Tn7 transposition target point sequence, a DNA fragment containing an AnpeNPV polyhedrin gene promoter sequence and an upstream gene partial sequence, and a DNA fragment containing a polyhedrin gene 3' terminal partial codingregion sequence and a downstream gene partial sequence, and linearized transfer vector plasmid DNA and linearized AnpeNPV genomic DNA are subjected to cotransfection with Tn-High Five cells, so that recombinant AnpeNPV is obtained. The recombinat AnpeNPV genomic DNA is transformed to escherichia coli, so that an AnpeNPV shuttle vector AnpeNPV-bacmid is obtained. The shuttle vector can be reproduced in the manner of plasmid DNA in the escherichia coli, can also be reproduced in the virus DNA manner in Tn-High Five insect cells and antheraea pernyi, and has infectivity.

The invention discloses a stage feedback loudspeaker box. The stage feedback loudspeaker box comprises a box body, wherein the contour of the cross section of the box body is shaped like a right trapezoid; two mid-range loudspeakers and two coaxial loudspeakers are arranged on an inclined panel of the box body; a sound amplifying module is arranged on a vertical back plate; the inclination angle of the inclined panel is between 40 degrees and 50 degrees; the width of a top flat plate is between 3cm and 30cm. The stage feedback loudspeaker box provided by the invention has the advantages that the box body adopts an irregular structural design, so that standing waves in the box body can be reduced, and the tone is cleaner; with the top flat plate, a singer can conveniently sit or place his/her feet on the box body, so that the interactivity with the audience is effectively enhanced; the inclined panel is arranged at an angle of 40 degrees to 50 degrees, so as to effectively ensure that the sound axis can be directly oriented to the ears of the singer, and achieve good radiation effects; the two coaxial loudspeakers and the two mid-range loudspeakers not only achieve a large volume, good layering effects and high appealingness, but also achieve an ampler frequency allocation of the loudspeaker box, smooth cohesion among high-frequency tones, middle-frequency tones and low-frequency tones, and a wide sound field, so that the overall tone quality is improved.

The invention relates to a method for painting on a glass surface by using acrylic paint. The method comprises the following steps that 1) a picture is painted on the glass surface by using the acrylic paint, and an acrylic paint layer is formed; 2) pigment ink is added to the acrylic paint layer after the water in the acrylic paint layer is volatilized completely; and (3) the water is dried; and the acrylic paint is composed of, by weight, 20-40 parts of methyl acrylate, 1-5 parts of ammonia water, 20-30 parts of nano color paste, 5-15 parts of calcium carbonate, 3-8 parts of magnesium carbonate, 3-5 parts of emulsifying agent, 0.5-2 parts of coupling agent, 20-40 parts of distilled water and 4-8 parts of linoleic acid. According to the method for painting on the glass surface by using the acrylic paint, the texture effects can change richly and naturally.

This invention discloses chemical splicing of full-length cDNA of western Nile virus, RT-PCR and real-time quantitative PCR techniques for detecting western Nile virus, and test kit for detecting western Nile virus. The full-length cDNA of western Nile virus can be used for detecting western Nile virus, developing anti-western Nile virus vaccines, and preventing western Nile virus infection. The test kit has such advantages as high sensitivity, easy operation and simple apparatus, and is suitable for high-throughput western Nile virus screen at frontier ports.

The invention discloses an enzyme linked immunosorbent assay instrument testing method for penicillium marneffei mycelia-phase in-vitro susceptibility testing. The light transmittance of a bacterium-containing diluent with an antibiotic medicinal liquid is tested by using an enzyme linked immunosorbent assay instrument in the penicillium marneffei mycelia-phase in-vitro susceptibility testing process. The densities are different in different bacterium growth situations, the light transmittances are also different, results are judged by using the enzyme linked immunosorbent assay instrument, apparent phenomena can be converted into values, and the operation can be relatively convenient and accurate; secondly, as an enzyme linked immunosorbent assay instrument pore plate is used, material consumption can be reduced; only a solution less than 1ml is needed in each pore, so that the amount of medicines is small, and waste can be greatly reduced; in addition, penicillium marneffei has infectivity, so that the pollution property can be further reduced because of the small use amount. Finally, normal growth of the penicillium marneffei can be not affected by the enzyme linked immunosorbent assay instrument, the operation is simple, convenient and rapid, and precise in-vitro susceptibility data results can be obtained.