158results about How to "Contribute to research" patented technology

The invention provides a preparation method of a V-based alloy metallographic sample, and belongs to the fields of heterogeneous alloy metallographic phase preparation. The method includes: subjecting a block V-based alloy sample to coarse grinding pretreatment, grinding with water step by step, preparing an electrolytic polishing solution, subjecting the sample to electrolytic polishing, subjecting the polished sample to cleaning under ultrasonic wave, wiping with alcohol, blowing the sample to dry the sample, preparing an electrolytic corrosion solution, subjecting the sample to electrolytic corrosion, washing the sample, wiping the sample with alcohol, blowing the sample to dry the sample, and performing metallographic observation. By adjusting experiment parameters and controlling the preparation steps of the V-based alloy metallographic sample, different metallographic phase shapes of the V-based alloy can be obtained, thus meeting observation requirements of different metallographic phases, reflecting comprehensive V-based alloy microstructure and providing experiment support for research of the V-based alloy.

The invention discloses a construction method of a literature history question and answer system based on a knowledge graph, which comprises the following steps of constructing the knowledge graph ofa literature history vertical field by taking structural data related to Chinese literature history as a basis and combining a literature history ontology structure created from top to bottom; designing a semantic analysis framework which comprises two user question analysis modules characterized in that one module is based on regularization and rules, and the other module is based on a neural network; organizing the results obtained through problem analysis into corresponding SPARQL query statements, and searching the corresponding results for in the constructed knowledge graph; organizing the result as a reply, and returning the reply to the user; designing a webpage side and a WeChat official account service as a window for interaction between the system and a user; designing a user uses a log and feedback collection module, wherein the related data is used for iteratively training a neural network model, and the generalization capability of the model is enhanced. According to the present invention, the natural language query of a user can be directly processed, an accurate result is returned, and the method plays an important role in improving the knowledge acquisition efficiency, promoting Chinese culture research and the like.

The invention relates to the biological field and discloses an animal source-free and serum-free culture medium of lymphocyte. The culture medium disclosed by the invention essentially consists of IMDM (Iscove Modified Dulbecco Medium), L-glutamine, sodium bicarbonate, recombinant human insulin, human transferrin, human serum albumin, 2-mercaptoethanol, N-acetyl-cysteine, lipid, amino acid, vitamin, microelement, ferric citrate, hydrocortisone, cholamine and non-essential amino acid. The serum-free culture medium disclosed by the invention has the advantages of clear chemical components, no animal source, no serum, safe and ideal culture cells; the instability caused by the doping of animal components and batches is avoided; the result of culturing lymphocyte shows that the total number of the cells and the cell phenotypes are normal; and the serum-free culture medium disclosed by the invention has a good industrial application prospect.

The invention discloses a centrifugal model testing system and method studying the common character of a composite foundation and a support structure. The centrifugal model testing system comprises a model box. Foundation soil is arranged in the model box. A support plate used for simulating a support pile is vertically embedded in the foundation soil. A plurality of foundation pile model piles are vertically arranged on one side of the support plate in the model box. A cushion layer is arranged on the top of the foundation soil in which the foundation pile model piles are embedded. The composite foundation is jointly formed by the foundation soil, the foundation pile model piles and the cushion layer. A digging area used for simulating digging is arranged on the other side of the support plate. Testing devices connected with a computer are distributed on the foundation pile model piles and the support plate. A load pressurizing device is arranged at the upper portion of the composite foundation. The whole testing system can be provided with a test system according to the requirements, and the test system is used for testing deformation displacement data and the like of the foundation soil in the composite foundation when a foundation pit is dug. When digging is conducted in the digging area, changes of force or displacement of the composite foundation or the support structure are studied, and practices can be better guided accordingly.

Provided is a simulation test system of a hydraulic support electro-hydraulic control system. The simulation test system comprises a small hydraulic support model, a hydraulic support display board, a simulation device, and a PC server, the small hydraulic support is provided with a plurality of sensors and used for displaying the movement of a hydraulic support and attitude of each part of the support, the hydraulic support display board is used for displaying the movement of the hydraulic support and the states of main sensors in a graphic visual manner, the simulation device is electrically connected with the sensors and used for receiving data of the sensors and performing preliminary processing of the data, and the PC server is electrically connected with the simulation device and used for receiving the data after preliminary processing by the simulation device and performing post-processing of the data and downloading on-site system operation data to a test system and performing reproduction of the on-site operation data and simulation of the system operating environment. According to the simulation test system, the on-site system operation data is collected and downloaded to the test system and is mutually integrated with the simulated test system via a control system, and the laboratory simulation tests in the condition of on-site environment are realized.

The invention discloses a cerebral cortex transcranial magnetic stimulation three-dimensional positioning navigation system and method. The navigation system comprises a fixing device, a navigation device and an auxiliary positioning device, wherein the fixing device comprises a fixer, and the navigation device is arranged above the fixing device and comprises a first motor and a second motor driven by the first motor; the auxiliary positioning device comprises a conformal mask and positioners arranged around the conformal mask, and positioning holes are formed in the conformal mask; the first motor and the second motor are connected to one controller. According to the navigation method, through a coordinate conversion method, the position of a nidus in a scintigram is made to correspond to the position in an actual coordinate system, then, the motors are driven for navigation, and a basis is laid for subsequent treatment. Through the device and method, the nidus can be accurately and rapidly positioned and stimulated, and good equipment and a good research idea are provided for transcranial magnetic stimulation treatment.

The invention discloses a method for measuring the inductance in the deep saturation state of a transformer. The method is used for solving the technical problem that in the prior art, no method is provided for simulating the running state of a transformer in a deep saturation state. According to the invention, the excitation surge current and the secondary side voltage of the transformer when thetransformer is in no-load and switched-on state are measured. Through the numerical integration and the calculation, the excitation curve of the transformer can be obtained. Meanwhile, the range of the excitation surge current is large, and the obtained excitation curve contains excitation characteristics in the deep saturation state. Therefore, the inductance of the transformer in the deep saturation state can be obtained by performing linear fitting on the obtained excitation curve. The operation state of the transformer in the deep saturation state can be studied by the engineering operation and maintenance personnel.

The invention relates to a method for realizing the parallel single-pixel imaging, which expands a basic method of the single-pixel imaging into a pixel array (image) sensor and greatly widens the application range of the single-pixel imaging method. The method comprises the following steps of firstly, calculating and positioning an observation area of each pixel on a pixel array by projecting and shooting a pattern mode of a Fourier slice; secondly, projecting a required pattern mode to the scene by using a projector, and shooting by using a camera to obtain a corresponding image; secondly, executing a parallel single-pixel imaging image reconstruction algorithm on all pixels on the camera image to obtain a corresponding image with projector resolution under each camera pixel. Due to the fact that the sparsity during the light transmission process is fully explored, the parallel single-pixel imaging method can greatly improve the imaging efficiency of a traditional single-pixel system. Meanwhile, the corresponding relation between the camera pixels and the projector pixels is established through the reconstruction result, and a good effect is achieved for further promoting the development of the optical imaging theory, the computer graphics and the computer vision.

The invention provides a fusobacterium nucleatum subsp.vincentii strain THCT14A3 for intestinal tract separation. The classification designation is Fusobacterium nucleatum subsp.vincentii, the preservation number is CCTCC NO:M 2019363, the preservation date is May 17th, 2019, and the preservation unit is China Center for Type Culture Collection. The 16SrRNA gene sequence of the strain is as shownin SEQID NO:1. The invention further provides a specific DNA sequence for identifying the fusobacterium nucleatum subsp.vincentii and a primer sequence corresponding to the specific DNA sequences. Thecolorectal cancer cell line and THCT14A3 co-culture result show that the THCT14A3 can significantly promote colorectal cancer cell proliferation. Therefore, the THCT14A3 provided by the invention canfacilitate micro-ecology research of intestinal tracts suffering from colorectal cancer.

The invention discloses an oral vaccine delivery system. W1 of W1 / O / W2 type nanoparticles is a vaccine medicine phase, an organic phase O is selected from PLGA and HP55, an external water phase W2 and a continuous water phase are selected from poloxamer 188, and a vaccine medicine is a double epitope-double adjuvant helicobacter pylori vaccine. The method adopts a polymer material having good biological safety and good compatibility to prepare the nanoparticles, a preparation process and a formula are optimized, the oral vaccine delivery system is non-toxic, free of any side effects, easy to manufacture and low in cost. In the field of biological medicines, the nanoparticles can be used for oral taking of a helicobacter pylori subunit vaccine, accordingly helicobacter pylori infection related diseases are prevented and treated, and huge economic benefits and social benefits are brought.

The invention discloses a preparation method of dendrobium officinale EST-SSR primers. The method comprises the steps of acquiring EST sequences of a dendrobium officinale root; screening dendrobium officinale EST sequences with SSR loci; designing primers; and verifying dendrobium officinale EST-SSR loci to finally obtain 117 pairs of dendrobium officinale EST-SSR labeled primers. The appropriate annealing temperatures of the 117 pairs of dendrobium officinale EST-SSR primers are found to range from 45 DEG C to 63 DEG C, the dendrobium officinale EST-SSR primers are applied to dendrobium officinale genome DNA after being subjected to DNA polymerase chain reaction, and 117 dendrobium officinale DNA sequences with microsatellite loci are obtained through amplification and sequencing confirmation. According to the preparation method, microsatellite molecular markers of dendrobium officinale are developed from the EST sequences, so that the preparation method has the advantages of simplicity, rapidness, stability, high efficiency and low cost. The method provides a powerful tool for molecular identification of dendrobium plants and is beneficial to further study on genetics and molecular biology of the dendrobium plants.

The present invention discloses a measurement method of the excitation curve of a transformer under a saturation condition. The invention aims to solve the technical problem that a method which can obtain the operating status of a transformer under a saturation condition through simulation does not exist in the prior art. According to the method of the invention, the excitation inrush current andsecondary-side end voltage of the transformer during the no-load closing of the transformer are measured; by means of numerical integration and calculation, the excitation curve of the transformer isobtained; since the range of the excitation inrush current is wide, the excitation curve contains the excitation feature of the transformer under a deep saturation condition, and therefore, information such as the parameter value of the transformer and the excitation feature of the magnetic core of the transformer under the saturation condition of the transformer can be obtained through the obtained excitation curve; and engineering and maintenance personnel can be facilitated to research the operation status of the transformer under the saturation condition.

The present invention relates to a mediating method of RNAi (ribonucleic acid interference) utilizing a lentiviral vector. The method comprises the following specific steps of: firstly carrying out homology analysis on the nucleic acid sequence of relevant viruses to find out conserved regions and designing siRNA (small interfering ribonucleic acid) corresponding to the conserved regions of the related viruses; and cloning the siRNA to an expression plasmid pLVTH, then co-transfecting 293T cells together with pCMV-dR8.91 and pMD2.G, collecting the supernatant of the transfected cells, carrying out ultracentrifugation, purification and concentration to obtain a high-titer lentiviral vector expressing the siRNA, then carrying out cotransduction into target cells together with LV-tTR-KRAB, and strictly controlling the acting time and dose of siRNA drugs utilizing tetracycline analogue DOX. Because of high transduction rate and a stringent control system, the mediating method can actually evaluate the in-vivo antiviral action of the siRNA and can track the whole process of drug interference action, thus having clinical application prospects.

The invention relates to a salt stress-induced specific promoter of a plant leaf and an application of the salt stress-induced specific promoter. A promoter sequence of an AtPGK2 gene is cloned from a model plant Arabidopsis, and then, in transgenic Arabidopsis, the promoter can drive a GUS (Glucuronidase) reporter gene to be specifically expressed in the plant leaf in a salt stress-induced form. By using the promoter provided by the invention, a promoter-target gene fused gene is established and obtained, and a transgenic plant having a target gene which can be specifically expressed in the plant leaf in a salt stress-induced form can be obtained by using the promoter-target gene fused gene into plant transformation. Therefore, the promoter not only is beneficial to research on the molecular mechanism of the plant responding to salt stress, but also is applied to plant genetic engineering so that the exogenous gene regulates specific expression of the target gene in the plant leaf in a way of salt stress induction, and meanwhile, the salt resistance of the plant is improved with a clear target. The promoter has a wide application value.

The invention relates to the field of gene engineering, and specifically discloses an application of taking a carboxylic acid amides bactericide resistant gene as an oomycetes conversion screening marker. According to the application, coding gene of a mutant protein, which can make oomycetes generate more than 100 times of resistance on a carboxylic acid amides bactericide, is taken as the screening marker and is used to screen the converter in the process of oomycetes conversion. The screening marker can solve the problem that the screening marker is single in genetic transformation of phytophthora capsici and helps the research on functions of gene of phytophthora capsici.

An electroporation apparatus comprises an elongated hollo member in order to provide a uniform electric field during electroporation, wherein specifically, electroporation is carried out b applying electric pulses through a couple of electrodes from both end parts of the elongated hollow member, after the hollow member is charged with fluid specimen including cells and material which would be injected into the cells.

The invention provides a system with a video monitoring function for carrying out research on circadian rhythms under the condition of exposure to microwaves. Various LD (light and dark) lighting systems for the research on the circadian rhythms are automatically implemented by a lighting system setting and illumination control module; a multi-processor working mode is adopted, a main processor notifies a video processor to process corresponding videos via a video processing function calling interface, and is connected with temperature, humidity and light intensity sensors by serial or I2C (inter-integrated circuit) interfaces, data can be acquired and stored, and whether the data exceed set threshold values or not can be judged; and the system supports two modes for controlling microwave radiation, microwave equipment is automatically controlled and measured by LXI standards in one mode, and a microwave generating circuit and a microwave measuring circuit are integrated in the system in the other mode. The system with the video monitoring function for carrying out the research on the circadian rhythms under the condition of exposure to the microwaves has the advantages that an automatic procedure and the lighting system implementation precision can be improved, and the complexity of manual operation is reduced.

The invention discloses a method for improving the imaging quality of Arabidopsis thaliana seeds, belongs to the field of biology, and particularly relates to the fields of methods for improving the imaging quality of plant seeds. The method aims at solving the problem that the three-dimensional image imaging quality of the Arabidopsis thaliana seeds is poor, and the images cannot be used for further analyzing internal parameters of the Arabidopsis thaliana seeds. The method for improving the imaging quality of the Arabidopsis thaliana seeds comprises the following steps that 1, a solution isprepared, wherein standard stationary liquid is prepared, and a cesium iodide solution is prepared; 2, Arabidopsis thaliana seeds are treated; 3, the Arabidopsis thaliana seeds are dried; 4, micro-calculation tomoscanner imaging of the Arabidopsis thaliana seeds is carried out; 5, a reconstructed picture is preprocessed. Arabidopsis seed images obtained through the method are subjected to Micro CTscanning, the result is clearer, the image quality is improved greatly, setting up of three-dimensional reconstruction of the Arabidopsis thaliana seeds is completely utilized, and reliable data is provided for calculation of the internal parameters of the Arabidopsis thaliana seeds.

The invention relates to a piperazine derivate and application thereof. The piperazine derivate takes N1-arylmethyl and N4-pyridyl piperazine as parent matrixes to modify the structure of pesticides of main pharmacophore of the N1-arylmethyl and N4-pyridyl piperazine, so as to obtain a type of novel compound. As shown by biological (insect disinfestation) activity determination: the novel compound designed and synthesized by the invention can effectively inhibit feeding and growth of crolepidopteron, and cause the crolepidopteron to die. The piperazine derivate not only is helpful to the further research of 5-hydroxytryptamine receptors of insects but also provides direction for creation of green pesticides with a novel functional mechanism.

The invention discloses a construction method for a fat body cell line of a chilo suppressalis larva. The construction method for the fat body cell line of the chilo suppressalis larva includes the following steps: (1) a cell primary culture solution and a cell subculture solution are prepared; (2) a chilo suppressalis larva is soaked in an ethanol solution, surface sterilization is carried out, and the larva body is cleaned and dried through absorption; (3) the larva body is placed in a wax dissection tray disinfected with ethanol, and the chilo suppressalis larva is dissected; (4) a fat body tissue block is placed in a cell culture bottle filled with an HBSS cleaning solution, the cell culture bottle with the fat body tissue block stands still, and the process of pipetting the HBSS and adding fresh HBSS is repeated for three times after fat body cells become adherent; (5) the cell primary culture solution is added, the cell culture bottle is placed into a non-illuminated cell culture box for culture through the night, and then the cell subculture solution is added; (6) the cell subculture solution is pipetted and replaced every 7 days till the culture bottle is filled with expanded and reproduced cells. The construction method for the fat body cell line of the chilo suppressalis larva provided by the invention facilitates chilo suppressalis researches under an isolated culture condition, so as to provide help for the research and development of chilo suppressalis prevention and control methods.

The invention provides a geomagnetic activity index correction method and system based on an MSISE00 atmospheric model, a medium and a terminal. The geomagnetic activity index correction method comprises the following steps: obtaining polar region energy injection data; calculating an average energy injection rate in every three hours based on the polar region energy injection data; calculating corrected geomagnetic activity indexes in every three hour intervals based on the average energy injection rate; and obtaining a corrected geomagnetic activity index corresponding to one natural day ora specified time interval based on the corrected geomagnetic activity indexes in every three hour intervals. According to the geomagnetic activity index correction method and system based on the MSISE00 atmospheric model, the medium and the terminal, the geomagnetic activity index input by the MSISE00 atmospheric model is corrected based on the statistical relationship and change rule of the polarregion energy injection and the geomagnetic activity index, so that the calculation precision of the atmospheric model is obviously improved.

The invention discloses a TDR (time domain reflection) technology based real-time test probe and device for water content of a triaxial sample of unsaturated soil. The probe and the device can be used for testing the local water content of the sample in a triaxial test of the unsaturated soil in geotechnical engineering. The probe comprises a base layer (1), multiple flexible conductors (2) and a connector (3), has certain flexibility, can be horizontally mounted on the cylindrical sample for the triaxial test of geotechnical engineering in a winding manner, is located between the triaxial sample (10) and a rubber membrane (4), is connected with a TDR tester (9) through a wire (8) and can monitor the water content of a mounting part at any time in a triaxial test process of the unsaturated soil. The problem that the water content of a local position cannot be acquired in the triaxial test process of the unsaturated soil in the geotechnical engineering is solved.

The invention provides a two-way loading fractured rock mass visual splitting grouting test device and method, and is suitable for the field of geotechnical engineering. The device comprises a splitting steel plate box, a side pressure loading part, a grouting part, a stress monitoring part, a separated hydraulic jack and a high-speed camera. Axial pressure is applied through the loading device, the lateral pressure is applied through water pressure by means of a lateral loading liquid bag, the scalability of the lateral loading liquid bag is combined with the characteristic that the rigidityof the axial pressure loading plate is small, and corner effect of a loaded fractured rock mass is eliminated. Furthermore, the whole transportation process of the split grouting slurry can be shot inreal time by adopting a transparent acrylic plate; rock samples are spliced according to different combination phases to form a fractured rock mass, and fractures with different inclination angles inthe rock mass are well simulated; the problems of the corner effect and invisibility in a loaded rock mass sample are solved, the fractured rock mass is assembled in a rock sample splicing mode to besubjected to a splitting grouting test in a two-way stress state, and important significance is achieved for researching fracturing grouting of the fractured rock mass.

The invention belongs to the technical field of engineering testing, and provides a wave velocity testing method and device by a single-hole method and a terminal device. The wave velocity testing method comprises the steps that firstly, a vibration source vibration signal generated by a surface vibration source device at a resonant frequency is obtained, a vibration source vibration waveform is generated, and the resonant frequency is the resonant frequency of a system formed by excitation blocks and strata in the vibration source device; vibration signals of measuring points in boreholes detected by a wave detector are obtained, and a corresponding measuring point vibration waveform is generated; then a wave train figure is obtained according to a mutual correlation analysis method, themeasuring point vibration waveform of the measuring points and the vibration source vibration waveform; and finally, the wave velocity testing results are obtained according to the wave train figure.The measurement of seismic waves is realized, the accuracy of seismic wave measurement in the strata is improved, and the study of the characteristics of foundation soil is facilitated.

The invention provides an on-site microwave rock breaking simulation system. The on-site microwave rock breaking simulation system comprises a microwave source, a wave guide tube, a circulator, a water load, a rock breaking test cabin, a microwave blocking and confining pressure loading system and a data acquisition system. The microwave blocking and confining pressure loading system is composed of a jack, microwave blocking front plates and microwave blocking side plates; all the microwave blocking front plates surround the other faces, except for a microwave irradiation face subjected to microwave irradiation treatment, of a rock sample located in the rock breaking test cabin, and all the microwave blocking side plates seal a gap between the microwave irradiation face of the rock sampleand the inner wall of the rock breaking test cabin on the plane where the microwave irradiation face of the rock sample is located; the microwaves only act on one face of the rock sample and correspond to a tunnel face in engineering practice. The invention further provides an on-site microwave rock breaking simulation method. The field microwave rock breaking working condition can be simulated more truly, and microwave rock breaking work can be guided more effectively in engineering practice.

The invention discloses an anti-oxidation experimental system for preparing nitrogen at room temperature during laser cladding. The anti-oxidation experimental system is composed of a constant-temperature nitrogen making machine and an anti-oxidation experimental box, wherein the constant-temperature nitrogen making machine comprises an air inlet pipe, a nitrogen-oxygen separation tank, an air outlet pipe, a glass box and an air valve switch, wherein the anti-oxidation experimental box comprises an air delivery pipe, a quartz plate, a box body, a hollow metal base and two small bases. According to the anti-oxidation experimental system for preparing nitrogen during room-temperature cladding disclosed by the invention, nitrogen separated from air can be filled into the anti-oxidation experimental box during laser cladding, so as to prevent a base body and a cladding layer from being oxidized; meanwhile, a few of changes of scientific research personnel and instruments in close observation and laser cladding process detection can be avoided, and thus further research of laser cladding is facilitated. The experimental system also has the advantages of being scientific and reasonable in structure design, simple to use, energy-saving, environmental friendly and the like.

The invention discloses a real-virtual interactive comprehensive experiment platform for a hydraulic mechanism arm, which comprises an upper computer master control system, a motion control system and a hydraulic servo experiment table; wherein the upper computer master control system receives the input control instruction of a user and is connected with the motion control system. Furthermore the upper computer master control system transmits a calculation result to the motion control system. The motion control system receives the instruction of the upper computer mater control system and drives the hydraulic servo experiment table to perform a corresponding action. The real-virtual interactive comprehensive experiment platform performs modular design on an execution mechanism and facilitates detachment. Three-dimensional virtual reality interaction technology is used for realizing real-virtual interaction between the hydraulic experiment table and the mechanical arm. Response characteristics of the servo valve and the execution mechanism can be tested. Execution accuracy of the control algorithm can be verified. The real-virtual interactive comprehensive experiment platform is suitable for research and development of the hydraulic mechanical arm.

The invention discloses a leptobotia elongate four-base repetitive unit simple sequence repeat (SSR) molecular marker establishing method, and an application thereof. The invention belongs to the field of molecular biological DNA marker technology and application. The SSR molecular marker establishing method comprises the steps of: a leptobotia elongate SSR four-base repetitive unit enriched library is established; screening and sequencing are carried out upon SSR sequence positive clones; according to the leptobotia elongate four-base repetitive core sequence, specific primers are designed on two ends; and through PCR amplification detection, 13 pairs of high-polymorphism SSR molecular markers are obtained by screening. The method provided by the invention is mainly applied in respects such as leptobotia elongate resource monitoring, genetic diversity analysis, genetic map construction, molecular-assisted breeding, and the like.

The invention belongs to the technical field of biological data analysis, and relates to a marker screening method based on a single variable and pair variables. As biological data has the advantagesof being high in dimensionality and small in sample size, variables are evaluated and selected through the simple and accurate decision rule, and classification and forecasting are important tasks ofbiological data analysis. For comprehensively evaluating the variables, a best split point of the single variable is calculated through information gain. A new variable is constructed through the bestsplit point, and the pair variables are established through the new variable and an original variable corresponding to the new variable. Meanwhile, variables of primal space generate variable pairs through combination two by two. All the pair variables are scored according to two scoring criterions, and are sorted in descending order according to scores, k pairs of variables which have the highest score and are not overlapped are selected to establish a fusion classifier. According to the marker screening method based on the single variable and the pair variables, the new variable is constructed through the single variable, the classification performance of the single variable and the classification performance of the pair variables can be evaluated through the same rules, and the and effective data processing method is provided.