The invention discloses a method for determining the oleaginous microalgae harvesting time by utilizing the
chlorophyll fluorescence parameter Pv / Fm. The method comprises the following steps: (1) carrying out conventional culture and
stress induction on oleaginous microalgae, namely carrying out conventional autotrophic culture on the oleaginous microalgae; culturing the oleaginous microalgae to mid-log phase, and inducing accumulation of
neutral fat under a stress condition; (2) detecting the
chlorophyll fluorescence parameter Fv / Fm, namely sampling in an oil-production inducing process, and detecting Fv / Fm; (3) detecting the content of
neutral fat, namely extracting
algae oil by
chloroform /
methanol, and detecting the content of the
neutral fat by means of a
thin layer chromatography method and
image analysis software; (4) determining the harvesting time, namely harvesting microalgae after the neutral fat in cells starts to be remarkably accumulated for 1-2 days, determining the Fv / Fm range after the neutral fat starts to be remarkably accumulated for 1-2 days to be the Fv / Fm harvesting range if the detected Fv / Fm and the neutral
fat content are remarkably related, and determining the harvesting time when the
algae solution Fv / Fm reaches the harvesting range in induction of neutral fat production under the same
stress induction. The method is simple and quick in the
chlorophyll fluorescence parameter Pv / Fm detection operation, has few interference factors, and is accurate and stable.