52results about How to "Long extraction time" patented technology

The invention relates to a method for preparing an antioxidative active extractive of sweet potato leaves, and belonging to the field of agriculture products processing. The method comprises the following steps: drying and crushing fresh sweet potato leaves; performing microwave assistant extraction after the decoloring and degreasing treatment is carried out; decompressing and concentrating extract in vacuum; chromatographing through a column, and absorbing by maroporous resin; collecting eluent; decompressing and concentrating the eluent in vacuum to obtain extract of the sweet potato leaves; and atomizing and drying the extract to obtain powdered extractive of the sweet potato leaves. The product is powdered, has the unique smell of the sweet potato leaves, contains 65 to 75 percent of total phenol, and contains chlorogenic acid and 3,5-O-dicaffeoylquinic acid. The method adopts mass waste sweet potato leaves to prepare the extractive with strong antioxidative activity, turns waste into wealth, is a green industry, has simple process, no need of larger equipment investment and high extracting rate of antioxidative active substances, and is applicable to industrial production.

The invention discloses a method for preparing active peptide of laver. The method is characterized by comprising the following steps of: drying and crushing porphyra yezoensis serving as a raw material, taking the part with particle size of between 150 and 200 meshes, adding water into the raw material according to a weight volume ratio of the raw material to water as 1 to 100g / mL and uniformly mixing; filling the material liquid into an ultrasonic cell disruptor for disruption and centrifuging to obtain supernatant; adjusting the pH value of the supernatant to be 7.0, adding papain for enzymolysis, inactivating, cooling and centrifuging to obtain enzymatic hydrolyzate; and performing ultrafiltration on the enzymatic hydrolyzate, concentrating filtrate and performing vacuum freeze dryingto obtain a freeze-dried product of the active peptide of the laver. The method has the advantages of more reasonable preparation process, simple process, high operability and high extraction rate; the prepared active peptide of the laver contains fewer impurities; and the biological activity is also kept to the greatest extent.

The invention relates to the field of agricultural fungicide, in particular to a botanical fungicide, an extracting method and the usage thereof. The botanical fungicide adopts extracts of plant raw materials such as sisal leaves, wild chrysanthemum, Chinese parasol leaves, ginger, bitter gourd or the like; the original preparation technology is improved, and the botanical fungicide is used for controlling agricultural diseases and has good environmental compatibility; the three-time extracting technique is adopted, and ultrasound-assisted extraction is applied, so that the maximum extraction capacity of active ingredient can be ensured; the technique is simple and seldom affects the environment, and the extracts have good effect on sterilization and bacteriostasis.

The invention relates to a method for extracting polysaccharides with cell immunocompetence from needle mushroom root waste materials and belongs to the field of biomedicine. The method comprises the steps of: drying and crushing needle mushroom roots to prepare ultrafine needle mushroom root powder, wherein the needle mushroom roots are the cut root parts as waste materials in a needle mushroom processing course; adding a proper amount of 85% ethanol to the crushed ultrafine powder to soak for 2 hours at room temperature; centrifuging to collect a precipitate; adding deionized water according to a ratio of water to material namely 28mL / g based on the raw material as a reference; carrying out ultrasonic treatment for 19.8 minutes at the power of 680W; centrifuging to collect a supernate; carrying out rotary evaporation on the supernate to obtain a proper amount of at room temperature; adding anhydrous ethanol the volume of which is 4 times that of a concentrated solution in the concentrated solution; precipitating for 12 hours at 4 DEG C; centrifuging to collect a precipitate; and placing the precipitate in an oven of 60 DEG C to bake so as to obtain crude needle mushroom root polysaccharide. An ultrasonic wave extraction technology is adopted, and the polysaccharide yield is 16.20%. Compared with the traditional hot water extraction, by using the method disclosed by the invention, the yield is improved by 70%, and the time is shortened by 92%.

The invention discloses a device and a method for active hole drilling and sealing. The device comprises a gas extraction pipe, a grout injection pipe, a grout return pipe, an inner-block grout disk, an outer-block grout disk, a sealing piston, a gravity hammer and a grout storage tank, wherein the bottom of the grout storage tank is provided with a grout injection opening which is controlled by a grout injection valve and a grout supplementing opening which is controlled by a grout supplementing valve. An inorganic flexible paste body is injected into a drilling space between the inner-block grout disk and the outer-block grout disk through the grout injection pipe, the grout return pipe is connected with the grout storage tank when the grout return pipe is subjected to grout return, the grout injection valve can be opened, the inorganic flexible paste body can be squeezed out by applying sustained pressure to the inorganic flexible paste body in the grout storage tank by the gravity hammer, cracks added around a drilling hole can be sealed and blocked, and the active hole drilling and sealing of the drilling hole can be realized. The device and the method, which are disclosed by the invention, have the advantages that the structure is simple, the operation is convenient, the cost is low, and the using effect is good.

The invention relates to a method for extracting cordycepin. The method includes the following steps of subjecting a cordycepin fermentation liquid to reduced pressure concentration, then performing ultrasonic extraction in combinations of microwaves and light waves respectively, and further subjecting an obtained extraction liquid to separation and purification to obtain the cordycepin. Compared with traditional extraction methods, the method has the advantages that by means of the method, the extraction ratio is improved, extraction time is saved, and purity is increased.

The invention relates to a method for extracting flavonoid compounds in mung bean hull, comprising the following specific steps: firstly grinding one part of mung bean hull, then performing extrusionand swelling treatment to the grinded mung bean hull, using organic solution to extract from the grinded puffed material, filtrating to collect extract, performing lyophilization concentration treatment to the extract and obtaining raw flavonoid compounds of mung bean hull. The invention combines the double screw extrusion and swelling technology and organic solvent extraction process to extract flavonoid compounds of mung bean hull, shortens the extraction time, improves the extraction rate, simplifies the operation and is applicable to industrialized production.

The invention discloses an automatic patent keyword extraction method, and belongs to the technical field of text retrieval. The method comprises the following steps of electronic document importing,retrieving and querying, title analysis, text analysis and keyword exporting, wherein text analysis comprises document generation, word recognition, word retrieval, word comparison, word acquisition,word displaying and word saving. According to the method, by extracting keywords in a patent text, patent retrieving and querying can be more easily completed.

The invention provides a method for detecting content of heavy metals in a plant sample, particularly a method for treating a to-be-detected sample in a sample pretreatment step by using dilute acid. Compared with a national standard method, the dilute acid method has wide application value, is easy and convenient to operate and rapid; and the national standard method is long in extraction time and easy to pollute and lose, and the dilute acid method is easy and wide to popularize. According to the method disclosed by the invention, heavy metal elements in vegetables are analyzed and measured, and the method has great significance for rapidly detecting the heavy metal elements in the vegetables.

The invention discloses a hole drilling and sealing device and method with continuous belt pressure. The device comprises a gas extraction pipe, a grouting pipe, a grout return pipe, grout blocking plates, a grout storing tank, a grout pressing plate and a spring, wherein the grout blocking plates are connected to the gas extraction pipe at equal intervals of 10m to enable the gas extraction pipe to be fed into a dill hole, and the outer grout blocking plate is made to be in the drill hole and in the position away from the opening of the drill hole for 1m. Inorganic flexible pasty fluid is injected to the drill hole space between every two grout blocking plates through the grouting pipe; when the grout return pipe performs grout returning, the grout return pipe and a grout supplementing valve on the lower portion of the grout storing tank are connected, an air receiving valve is opened, and continuous air pressure is applied to the inorganic flexible pasty fluid in the grout storing tank; when fractures around the drill hole increase, the inorganic flexible pasty fluid is continuously pressed into the fractures around the drill hole by the air pressure in the well. When the inorganic flexible pasty fluid in the grout storing tank is totally pressed into the drill hole, a pressure relief valve is opened, and a grout pressing plate ascends under the action of the spring. Due to the fact that the inorganic flexible pasty fluid is injected into the grout storing tank again through a grouting valve, the drill hole is effectively sealed through the continuous belt pressure.

The invention relates to a method for extracting effective component of traditional Chinese medicine, with dynamic overflow continuous reverse-flow method, wherein said method uses several extractors, while each extractor works independently and they use active overflow method, to transfer liquid continuously. Compared with traditional method, the invention improves extracting speed, reduce solvent consumption, reduce following energy consumption and improve efficiency. The invention can reduce extracting temperature, etc. And the invention comprise several pots, heater, condenser, tubes, pump and value which control the active overflow between pots, and the meter element controlling the balance of whole device.

The present application relates to a method for simultaneously detecting four isomers of resveratrol in peanut by using ultra performance liquid chromatography during the operation procedure and accurately controlling the detection conditions. The method of the invention can achieve continuous sample injection, and perform sample analysis in batch. The detection time for each sample is only about 10 min, which greatly improves the detection efficiency. Further, the ultra performance liquid chromatography has high sensitivity and a low detection limit. The method of the invention adopts ethanol extraction under heating in the earlier stage of extraction, and controls the temperature of the extraction, so as to achieve effective extraction of four isomers of resveratrol, time saving, and efficient extraction and separation.

The present invention provides a method for preserving nucleic acid on the solid surface, and a preservation solution thereof, and relates to a nucleic acid extraction and preservation solution, which comprises a buffer, a non-ionic surfactant, a biological surfactant and bovine serum albumin. According to the present invention, with the preservation solution, cells and other samples are placed into the nucleic acid extraction and preservation solution to make DNA be stably preserved in the nucleic acid extraction and preservation solution so as to save the nucleic acid extraction time of the laboratory; according to the existing detection kit, the sample is generally preserved in the nucleic acid preservation solution, the extraction is performed by using other extraction methods after the sample returns to the lab, the whole process exceeds 1 h, and if the number of the sample is more, the extraction is substantially prolonged, such that the extraction rate determines more than or equal to half of the detection time of the sample; with the technical scheme of the present invention, the cells and other samples can be placed into the nucleic acid extraction and preservation solution to make DNA be stably preserved in the nucleic acid extraction and preservation solution so as to save the nucleic acid extraction time of the laboratory.

The invention discloses a method for preparing fructus amomi volatile oil. The method comprises the following steps of taking dry fructus amomi medical material, and crushing into crude powder of 20 meshes-50 meshes after weighing, placing into a 100-mesh filter bag, and filling the filter bag into a material column of a subcritical extraction kettle; adding 1,1,1,2-tetrafluoroethane which is 4-20 times the weight of the fructus amomi into the extraction kettle as an extracting agent, extracting for 20 minutes-60 minutes under the conditions that the pressure is 0.3-1.6 Mpa and temperature is 20 DEG C-50 DEG C, placing extracting liquor into a separating kettle, evaporating and recycling the extracting agent for use under pressure of 0.04-0.1 Mpa, wherein residual liquor is fructus amomi volatile oil extracting liquor; repeating the extracting process twice-five times, combining and layering the fructus amomi volatile oil extracting liquor each time, and then carrying out oil-water separation, wherein the obtained oil phase is fructus amomi volatile oil, and yield of the fructus amomi volatile oil is not less than 90%. The fructus amomi volatile oil prepared by the method is high in yield, low in cost, good in perception and suitable for large-scale industrial production of the fructus amomi volatile oil.

The invention discloses a technique for extracting and purifying oleanolic acid and ursolic acid from sweet tea, which includes the following steps: absolute ethyl alcohol is adopted for ultrasonic extraction and suction filtration; combined extract is concentrated, so that an extract is obtained, chloroform is used for dissolving the obtained extract, and after recovering chloroform by concentration, a brown extract is obtained; 5 percent of sodium hydroxide is used for carrying out alkali dissolution and acid precipitation on the extract, and a method, such as silica gel column chromatography separation, is carried out on precipitate, so that high-purity oleanolic acid and ursolic acid are obtained; by adopting a high-performance liquid chromatography method to carry out content determination, the purities of both oleanolic acid and ursolic acid are 90 percent or more, the yield of oleanolic acid is 20mg / 100g or more, and the yield of ursolic acid is 90mg / 100g or more. The technique disclosed by the invention has the characteristics of simple needed equipment, convenience in operation, short extraction time, high extraction efficiency, energy saving, material saving, mild conditions, no active component destruction and the like, provides a feasible source for the extraction of oleanolic acid and ursolic acid from the natural plant, increases the added value of sweet tea, and has important application value and economic and social benefits.

The invention discloses a panel gas treatment method based on treelike oriented long drilled holes gradually increased step by step in zones. The treelike oriented long drilled holes are arranged in the length direction of a panel, and the whole panel is covered by using main holes; planar branch holes are arranged by adopting a partition step-by-step gradual increasing mode and used for coveringplanar extracting blind areas caused by the drilling trajectory control precision, and the drilled holes are encrypted; section extracting blind areas caused by parting layers are covered by upper-layer branch holes and lower-layer branch holes; and the method not only can perform the overall ahead pre-extraction treatment on gas in the panel, but also can perform extraction treatment on extractedpressure relief gas during coal seam recovery, so that such difficulties as low progressive extraction efficiency, easy generation of extraction blind areas by the drilling trajectory control precision and the parting layers and unbalanced extraction due to arrangement of the drilled holes at equal intervals, the no-blind-area overall balanced extraction of the panel is realized, the coal mine gas treatment efficiency and effect are improved, and the safe and efficiency mining is guaranteed.

The invention discloses green and healthy cakes. The green and healthy cakes are prepared form the following raw materials: low-gluten flour, highland barley flour, eggs, avocado oil, gentiooligosaccharide, lactitol, skimmed milk powder, green juice, almond kernels, table salt, kefir, lysozyme and water. The green and healthy cakes provided by the invention are rationally proportioned; and the green and healthy cakes are fine, smooth and soft in texture, mellow in refreshing aroma, bright green in color and luster, balanced in nutrition and easy to absorb; the fat contents of the green and healthy cakes are reduced by 41%; the green and healthy cakes are free of sucrose; and the green and healthy cakes are capable of promoting metabolism, expelling toxicants, keeping youth, enhancing immunity, resisting oxidation, resisting senescence and revisiting cancer. Enzymatic hydrolysis and fermentation are carried out on the green juice sources, so that the juice yield is improved by 13%; and the prepared green juice is sour-sweet in taste and naturally green in color, and has the effect of keeping the body healthy. The green and healthy cakes are steamed with separated water, so that harmful substances are reduced, and volumes of the cakes do not change before and after cooling; and the prepared cakes are fine, smooth and soft in texture as well as non-greasy in taste. The highland barley powder is puffed, and ultrafine crushing is performed on the puffed highland barley; thus, the prepared highland barley powder is high in nutrient content and capable of promoting gastrointestinal functions as well as increasing water retaining capacity of the cakes; moreover, the extraction time of the high land barley is shortened to five sixths of the original extraction time, and the powder yield is improved by 14%.

The invention discloses a method and device for extracting and scattering tobacco stems synchronously. The method comprises the step one of carrying out feeding, wherein the tobacco stems and solvent are fed into a storage tank according to the set proportion; the step two of carrying out stirring, wherein a push type paddle stirrer is started, and the materials in the step one are stirred and mixed uniformly to obtain a tobacco stem mixture; the step three of carrying out scattering while carrying out extracting, wherein a scattering machine is started, the tobacco stem mixture in the storage tank enters the scattering machine under action of stirring and driving force of the push type paddle stirrer, and then flows back to the storage tank through a circulating pulp pipe by utilizing the centrifugal pumping action generated by a rotor and pulp separating blades, so that the materials are extracted and scattered in a circulating mode; the step four of carrying out secondary extracting, wherein the scattering machine is closed for carrying out secondary extraction. While extracting and scattering are completed, the purposes of high extracting efficiency, good scattering quality and low energy consumption are achieved.

The invention discloses a method and system for extracting sludge protein by a focused pulsed electric field. According to the method and system, sludge that is homogenized and flowable flows throughdifferent electric field zones, generated by the staggering of cathode and anode plates, in a focused pulsed electric field sludge reactor; the treated sludge flows out from the reactor, and then is circulated and returned by a circulating pump; after thorough treatment, separation is performed through a high-speed centrifuge; the pH value is adjusted to precipitate protein; and then protein solidis obtained by a freezing vacuum belt type filter. The method and the system utilize electroporation effect of the focused pulsed electric field on biological cells to break sludge cells so that protein in the biological cells can be effectively released. The method and the system have advantages of low energy consumption, fast response, small consumption of pharmaceuticals, and capability of preparing the extracted biological protein with high purity and no side effect.

The invention provides a huperzine A production method suitable for industrial production and relates to a huperzine A production method. The huperzine A production method comprises the following specific steps of pretreatment of raw materials, extraction, centrifugation, ultra-filtration, nano-filtration, PH value regulation, concentration, microwave drying and smashing. The huperzine A production method adopts a membrane technology for separation and purification. Compared with other purification modes such as macroporous resin adsorption and solvent extraction, the huperzine A production method has the advantages of being safe, environmentally friendly and capable of saving energy, only using a pure water solvent, not adopting an organic solvent and the like, the operation is simple, the product is safe, environmentally friendly, good and inexpensive, and continuous large-scale industrial production can be achieved.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a preparation method of Qingjin oral liquid. According to the method, dynamic countercurrent extractionis adopted, wherein parameters of dynamic countercurrent extraction are selected; twelve medicinal materials in the Qingjin syrup are extracted; a single factor and an orthogonal experiment are combined and the bergenin transfer rate is used as an index, multi-index weighting is carried out to determine key factors. The optimal process parameters of the factors such as the medicinal material granularity, the extraction frequency, the material-liquid ratio, the extraction time, the extraction temperature and the like are determined. According to the present invention, the dynamic countercurrent extraction technology is adopted, such that the extraction temperature is low, the solvent consumption is low, the extraction efficiency is high, the energy consumption of the subsequent working section is low, the product quality is improved, and the product technical level can be significantly improved.

The invention relates to a method for extracting flavonoid compounds in mung bean hull, comprising the following specific steps: firstly grinding one part of mung bean hull, then performing extrusion and swelling treatment to the grinded mung bean hull, using organic solution to extract from the grinded puffed material, filtrating to collect extract, performing lyophilization concentration treatment to the extract and obtaining raw flavonoid compounds of mung bean hull. The invention combines the double screw extrusion and swelling technology and organic solvent extraction process to extract flavonoid compounds of mung bean hull, shortens the extraction time, improves the extraction rate, simplifies the operation and is applicable to industrialized production.

The invention provides a kit and a method for extracting circulating DNA from plasma. The kit provided by the invention comprises a DNA one-step reaction binding buffer solution, a washing solution, an eluent, and an independent aseptic packaged circulating DNA purification extension tube used in combination with a DNA binding column. The kit and the method provided by the invention can ensure that plasma samples having different starting volumes pass through the DNA binding column at a time, can effectively avoid cross-contamination between samples while rapidly and efficiently extracting high-quality circulating DNA, and have obvious advantages in comparison with common kits and methods.

The invention discloses a preparation method for black soyabean seed coat anthocryanin, which comprises following steps: 1. peeling the black soyabean seed coat as material; 2. smashing the seed coat into fine with micronizer to treat with microwave machine; 3. using 60~80% ethanol solvent to leach the said anthocryanin; 4. condensing the said condensing liquid in vacuum till the concentration of solid in extract liquid more than 40%; 5. degreasing, absorbing with macropore resin and desorbing with 65~75% ethanol, and obtaining the purified extract liquid; 6. repeating the vacuum condensation till the concentration of solid in extract liquid more than 60%, and freeze drying to obtain the objective product with well stability. This invention is simple.

The invention relates to the technical field of processing of traditional Chinese medicine processing and provides a preparation method of nigrum fruit formula granules. The preparation method comprises the following steps: carrying out edulcoration and crushing on fresh nigrum fruits, so as to obtain medicinal slices; carrying out alcohol extraction twice and water extraction twice on the medicinal slices; carrying out concentration; and carrying out vacuum microwave drying so as to obtain a mixed material, adding dextrin, and carrying out granulation, so as to obtain the nigrum fruit formula granules. The alcohol extraction and the water extraction are carried out twice, the concentrations, time and temperatures of the alcohol extraction and the water extraction are controlled, and then the vacuum microwave drying is carried out, so that the content transfer rates of solasonine and solamargine in the nigrum fruit formula granules are increased, and each 1g of the prepared nigrum fruit formula granules is equal to 5.0g of the weight of a medicinal slice.

The invention discloses a production process of dried orange peel cool tea. The production process includes the steps of: crushing snow pears and placing the snow pears with lalang grass rhizome and dried orange peel into an extraction tank; adding water and heating the extraction tank to boil water in the extraction tank, and maintaining the water boiled for 30-120 min; during extraction, intermittently starting a circulating pump to circulate extract liquid, and after the extraction is finished, filtering the extract liquid for later use; repeatedly extracting the extract liquid for one time; mixing the two extract liquids and adding oligosaccharide, stirring and dissolving the liquid and filtering the liquid to obtain the dried orange peel cool tea. The method is high in extraction rate of effective components during extraction since that: 1) extraction time is prolonged; 2) water for extraction is circulated; and 3) the drug residues are secondarily extracted. In addition, in the process, use amount of Chinese herbal medicines and the snow pears is high and the cool tea is sugar-free and is low in water addition ratio, so that the cool tea has significant lung moistening and cough relieving effects and also has the effects of relaxing bowel and removing toxin.

The invention discloses a traditional Chinese medicine composition for treating piglet diarrhea and a preparation method of the traditional Chinese medicine composition, belongs to the technical field of veterinary medicines, and aims to solve the problems that existing antibiotic medicines are easy to generate drug resistance and existing traditional Chinese medicines are single in treatment disease. The traditional Chinese medicine composition comprises the following raw materials: codonopsis pilosula, astragalus membranaceus, coptis chinensis, scutellaria baicalensis, turmeric, clove, pokeberry root, phoenix-tail fern, medicated leaven, fried coix seed, fructus psoraleae, purslane, dandelion and liquorice. The preparation method comprises the following steps: crushing the raw materials into particles, mixing codonopsis pilosula, astragalus membranaceus, dandelion, coptis chinensis, radix scutellariae, fried coix seeds, fructus psoraleae and purslane according to the formula amount, and carrying out a reflux extraction method; mixing the other raw materials according to the formula amount, and extracting by a decoction method; and mixing the extracting solutions, concentrating to obtain an extract, adding auxiliary materials in an equal amount, grinding, sieving, and drying to obtain the traditional Chinese medicine composition. The traditional Chinese medicine composition disclosed by the invention can be used for replacing antibiotic medicines, is effective for diarrhea of piglets caused by various reasons, and can be used for effectively improving the immunity of the piglets.