44results about How to "Not easy to extract" patented technology

The invention relates to a method for grinding and protecting cephalopod otolith. The method comprises the steps of extraction, soaking, placing, resin embedding, cutting, fixing on a glass slide, abrading, polishing, preservation and the like. The method can solve the problems that the cephalopod otolith is small and not easily ground, the otolith is easily broken in the grinding process and the textures are easy to fade after polishing; and the otolith textures can be kept for long time.

The invention relates to a method for detecting circulating tumor cells: collecting human body fluid samples, fixing with formaldehyde to increase cell membrane permeability, adding cells into several groups of PCR multiplex tubes, each tube is added with a reverse transcription primer carrying ID1, and reverse transcription After the cDNA molecules are recorded, the cells are collected and mixed into another set of PCR multiplex tubes. Each tube is added with an extension primer carrying ID2 and the 3' end can specifically recognize the 3' end of the cDNA molecule, and the extension reaction is carried out after sequence complementation. , cells were harvested and lysed, and extension products were pre-amplified by PCR primers. High-throughput sequencing to analyze the combination of ID1:ID2. Its advantages are: simultaneous reverse transcription and PCR amplification of different markers can accurately identify circulating tumor cells and circulating tumor cells with EMT, and can be used in combination with other technologies to detect the few circulating tumors in body fluid samples to the greatest extent. cells and other non-humoral rare cells.

A method for increasing the permeability of an inert gas and extracting gas under the coupling action between temperature and pressure, belongs to a method for extracting the gas. A permeability increasing hole is formed in the middle of a workface coal bed; gas extraction holes are formed on vertexes of an equilateral triangle with the permeability increasing hole as a center; after the gas extraction holes are sealed, the gas is extracted; the inert gas under the coupling action between temperature and pressure is injected into the permeability increasing hole one by one; under the coupling action of temperature and pressure of the nitrogen, a small-size clearance of a coal body is pressed by the inert gas to be opened, so that an original crack in the coal body is stretched, the stretched crack further extends and no protrusion by induction occurs; 90 percent of adsorption gas in the coal body absorbs energy and the separating speed of the gas from the coal body is increased, so that gas which is difficult to desorb in the coal body is converted into free gas which is convenient to quickly extract in a large flow; and a permeability increasing medium is gas, and the gas subjected to permeability increase can be exhausted from a crack channel without blocking the gas flowing channel, so that the conditions for the extraction of the gas is provided. The method has the advantages of simplicity in operation, favorable effect, high extraction efficiency of the gas and applicability.