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Chenopodium quod fermentation object and application thereof

A technology of fermented products and quinoa, applied in the directions of drug combination, plant raw materials, cosmetic preparations, etc., can solve the problem of non-whitening effect, etc., and achieve the effects of inhibiting the formation, increasing the content of phenolic substances, and reducing the formation of melanin.

Pending Publication Date: 2020-04-03
BIOPHARM RES & DEV CENT JINAN +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quinoa added to these cosmetics is not a fermented product, but mainly uses the high fiber content of quinoa, and is not used for whitening effect

Method used

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  • Chenopodium quod fermentation object and application thereof
  • Chenopodium quod fermentation object and application thereof
  • Chenopodium quod fermentation object and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0039] The preparation method of described a kind of quinoa fermented product, comprises the following steps:

[0040] (1) Pulverize the shelled quinoa seeds and sieve;

[0041] (2) Weigh quinoa flour, add deionized water and anhydrous calcium chloride (for improving the thermal stability of α-amylase), stir, and heat and gelatinize;

[0042] (3) Add α-amylase, heat and liquefy at 70-85°C, after liquefaction, cool and adjust pH;

[0043](4) Heating to 55-60°C, adding glucoamylase, keeping warm for saccharification for 2-5 hours, after saccharification, cooling, adjusting pH, autoclaving and cooling;

[0044] (5) Activate the fermentation strains with sterile saccharification solution for 15-30 minutes, then inoculate the activated strains into the sterile saccharification solution according to the proportion, and culture them statically in a biochemical incubator at a temperature of 30-40°C. When the pH value reaches a certain range, stop the fermentation;

[0045] (6) Afte...

Embodiment 1

[0061] Example 1 Preparation of quinoa fermented product

[0062] The present embodiment provides a kind of preparation method of quinoa fermented product, comprises the following steps:

[0063] (1) Pulverize the shelled quinoa seeds (white quinoa produced in Hainan) and pass through a 20-mesh sieve;

[0064] (2) Weigh 20g of quinoa flour, add 200mL of water and 0.04g of anhydrous calcium chloride, stir, and heat to gelatinize;

[0065] (3) Add α-amylase (Guangdong Baiyi Food Ingredients Co., Ltd., specification: 1000U / g) 0.44g, and keep it at 70-75°C for 30 minutes to liquefy; after liquefaction, cool and adjust to pH 4.0;

[0066] (4) Heat to 55-60°C, add 0.06g of glucoamylase (Guangdong Baiyi Food Ingredients Co., Ltd., specification: 100000U / g), keep warm for saccharification for 2 hours; after saccharification, cool, adjust pH to 6.2, and pressurize at 117°C After sterilizing for 20 minutes, sterilize by ultraviolet light on a clean bench and cool down;

[0067] (5) A...

Embodiment 2

[0071] Embodiment 2 quinoa fermented product is to the determination of tyrosinase activity

[0072] 1. Solution preparation:

[0073] ①Phosphate buffer (pH=6.5): Take 0.68g of dipotassium hydrogen phosphate, add 15.2mL of 0.1mol / NaOH solution, and dilute to 100mL with water.

[0074] ②Mushroom tyrosinase solution (acting as an oxidizing agent): take 1mL of mother solution (1000U / mL), add 9mL of phosphate buffer, and dilute to 100μ / mL.

[0075] ③L-Tyrosine solution: Take 0.030g of tyrosine, first dissolve it with 10mL of 0.1mL hydrochloric acid solution, then add buffer to make the volume to 100mL to obtain a 0.3mg / mL solution.

[0076] 2. Sample determination

[0077]

[0078] After adding the ①③④ solution, put it in a 37°C water bath for 10 minutes, add the ② solution and put it in a 37°C water bath for 15 minutes, then immediately measure the OD value at 475nm with a spectrophotometer (model 722N, Shanghai Jingke Industrial Co., Ltd.).

[0079]

[0080] see results...

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PUM

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Abstract

The invention discloses a chenopodium quod fermentation object and application thereof in cosmetics. The chenopodium quod fermentation object disclosed by the invention is manufactured by taking shelled chenopodium quod particles as a raw material and taking lactic acid bacteria and / or saccharomycetes as a fermentation culture through a biological fermentation method. A preparation process is that smashed chenopodium quod powders are evenly mixed with water, after a series of working procedures, including liquification, saccharification and the like, sealing is carried out after the culture is added, and a fermentation product is subjected to centrifugal filtration to obtain chenopodium quod fermentation liquor. The chenopodium quod fermentation object disclosed by the invention can effectively resist injury to skin cells by free radicals, the melanin generation of melanin cells is reduced, and the chenopodium quod fermentation object performs functions of regulating skin hyperpigmentation and brightening skin. A series of product obtained in a way that chenopodium quod fermentation object is compounded with other active ingredients, including oligopeptides and collagen, has the obvious efficacy on whitening and brightening the skin.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and cosmetics, and in particular relates to a quinoa fermented product and the application of the quinoa fermented product in cosmetics. Background technique [0002] Quinoa (Chenopodium quod), also known as quinoa, is a plant of the genus Chenopodium in the Amaranthaceae family. It is native to the plateau region of the Andes Mountains in South America. It has a planting history of about 5,000 to 7,000 years. It is the main food highly respected by the local Incas. one of the crops. The Food and Agriculture Organization of the United Nations (FAO) believes that quinoa is the only single plant that can meet the basic nutritional needs of the human body, and recommends quinoa as a complete nutritional perfect food that can best meet human beings. The grain protein content of the same quinoa planted in Hainan at low latitudes is higher than that of quinoa planted in Shanxi at high latitudes. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/08A61Q19/02A61K36/21A61P39/06A61P17/00
CPCA61K36/21A61K2236/19A61K2800/85A61Q19/02A61Q19/08A61K8/9789A61P17/00A61P39/06
Inventor 项琪黄亚东肖巧学
Owner BIOPHARM RES & DEV CENT JINAN
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