The invention belongs to the technical field of radish sprouting cultivation and particularly provides a cultivation method of a high-yield radish sprouting having high phenolic substance content. Thecultivation method comprises the following specific steps that 1, irradiation treatment is conducted on radish seeds by using 60 Co-gama rays, the radish seeds are immersed in a magnesium chloride solution for soaking, constant stirring is performed, draining is performed, the seeds are taken out and are flushed with clear water for 3-4 times, the seeds are immersed in a warm glucose nutrient solution for heat preserved soaking, draining is performed, the seeds are taken out, and treated radish seeds are obtained; 2, the treated radish seeds are sowed in a seedling raising medium, the sowingdepth is 1.1-1.3 cm, and the obtained seedling raising medium is prepared from the following raw materials: sunflower seed shells, pear residue, leaf mold, hyodeoxycholic acid, chelerythrine, perillapolysaccharide and thistle polysaccharide. Vigorous growth of stems and leaves of radish sprouts can be effectively promoted, the bitter taste of the radish sprouts can be reduced, the content of total phenols can be increased, the fresh weight of upper-portion parts can be increased, high-yield and high-quality radish sprouts are produced, human health is effectively promoted, and the economic benefits of radish sprouts are increased.