The present invention discloses a preparation method of a liver-protecting and intestine-protecting fermented granule for litopenaeus vannamei. Flour, corn germ meal, fish oil, soybean oil, soybean phospholipid oil, fish meal, shrimp shell powder, shrimp paste, soybean meal, fermented soybean meal, rapeseed meal, cottonseed meal, monocalcium phosphate, fine stone powder, a vitamin premix, a mineral premix, lysine, methionine, an antioxidant and a mildew preventive are mixed according to a certain proportion, the obtained mixture is subjected to high-temperature and high-pressure granulation, when a temperature is reduced to 30-40 DEG C, a mixed microorganism solution prepared from enzyme-producing bacillus licheniformis, bacillus subtilis, lactobacillus plantarum, lactobacillus acidophilusand saccharomyces cerevisiae in proportion is used for inoculation, the mixed microorganism solution is filled into a breathing bag for fermentation for 96-168 h and the liver-protecting and intestine-protecting fermented granule for litopenaeus vannamei is prepared. The feed is rich in various probiotics such as lactic acid bacteria, bacillus and saccharomycetes, also rich in small peptides, amino acids and lactic acid, good in a food calling performance, comprehensive in nutrition, high in content of microorganism enzymes, good in digestion and absorption, low in content of anti-nutritionalfactors, capable of protecting liver and intestines and strong in growth promoting capacity.