Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent

A feed raw material and starter technology, which is applied in animal feed, animal feed, application, etc., can solve the problems of mixed strains, single species, and poor effect, and achieve the effects of high number of viable bacteria, increased nutritional value, and improved taste

Inactive Publication Date: 2013-02-20
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The strains used in the existing starter cultures vary from good to bad, and the species is single. Most of them are fermented by Aspergillus fungi. Compared with the fermentation of compound strains, the fermentation effect is poor.
At present, there are many types of starters used for fertilizers on the market, but there are only a few compound starters for agricultural and sideline products.

Method used

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  • Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent
  • Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent
  • Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 Candida tropicalis powder

[0026] Candida tropicalis was purchased from China Research Institute of Food and Fermentation Industry.

[0027] Seed medium: 1.0% yeast extract powder, 2.0% peptone, 2.0% glucose.

[0028] Culture conditions: 28°C, 180rpm, 24h.

[0029] Candida tropicalis medium: 1.0% yeast extract powder, 4.0% glucose, 1.0% peptone, 0.2% potassium dihydrogen phosphate, 0.03% magnesium sulfate.

[0030] Culture conditions: 28°C, 150rpm, 72h.

[0031] Gained fermented liquid is centrifuged with high-speed refrigerated centrifuge, centrifugal condition: 6000rpm, 4 ℃, 10min. After centrifugation, the supernatant was discarded to obtain the bacteria sludge. Mix 10% glycerin and 0.85% normal saline at a volume ratio of 1:1, dilute and stir the sludge and mix well, then add 10% imported skim milk powder and mix well. First put it in a -80°C ultra-low temperature refrigerator to freeze, then put it in a vacuum freeze dryer to d...

Embodiment 2

[0032] The preparation of embodiment 2 bacillus licheniformis bacterial powder

[0033] Bacillus licheniformis strains were purchased from China Research Institute of Food and Fermentation Industry.

[0034] Seed medium: 0.5% beef extract, 1.0% peptone, 0.5% sodium chloride.

[0035] Culture conditions: 37°C, 180rpm, 24h.

[0036] Bacillus licheniformis medium: 3.0% brown sugar, 1.0% corn flour, 3.0% bean cake powder, 0.25% urea, 0.15% sodium dihydrogen phosphate.

[0037] Culture conditions: 37°C, 180rpm, 24h.

[0038] The obtained fermentation liquid was centrifuged with a high-speed refrigerated centrifuge, and the centrifugation conditions were: 5000 rpm, 4° C., 10 min. After centrifugation, the supernatant was discarded to obtain the bacteria sludge. Stir the sludge with 5.0% sucrose, 1.0% sodium glutamate, and 1.0% ascorbic acid aqueous solution, and then add 50% imported skim milk powder. First put it in a -80°C ultra-low temperature refrigerator to freeze, then pu...

Embodiment 3

[0039] The preparation of embodiment 3 Lactobacillus plantarum bacteria powder

[0040] Lactobacillus plantarum strains were purchased from China Food and Fermentation Industry Research Institute.

[0041] The seed medium is commercial MRS broth (special medium for lactic acid bacteria).

[0042] Culture conditions: 37°C, 100rpm, 12h.

[0043] Lactobacillus plantarum fermentation medium: 3.5% peptone, 6.0% beef powder, 0.50% diammonium citrate, 3.5% sucrose, 1.0% yeast extract powder, 0.4% dipotassium hydrogen phosphate, 0.1% sodium acetate, 0.02% magnesium sulfate, 0.005% manganese sulfate, 0.05% Tween 80.

[0044] Culture conditions: 37°C, 50rpm, 12h.

[0045]The obtained fermentation liquid was centrifuged with a high-speed refrigerated centrifuge, and the centrifugation conditions were: 5000 rpm, 4° C., 10 min. After centrifugation, the supernatant was discarded to obtain the bacteria sludge. Stir the sludge with 5.0% sucrose, 1.0% sodium glutamate, and 1.0% ascorbic ...

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Abstract

The invention discloses a probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and a preparation method of the probiotics leavening agent. The leavening agent comprises composite probiotics powder and auxiliary materials. The composite probiotics powder is candida tropicalis powder, aspergillus niger agent, bacillus licheniformis powder and lactobacillus plantarum powder. The probiotics leavening agent can be used for cake dreg type feed raw material fermentation, can effectively degrade anti-nutritional factors in cake dregs, improves taste and protein quality of cake dreg feed, and accordingly effectively improve use ratio of the cake dregs in various livestock feed.

Description

technical field [0001] The invention belongs to the technical field of feed, and relates to a probiotic starter suitable for fermented cake feed raw materials and a preparation method thereof. Background technique [0002] The by-products of oil extraction from fat-rich legume seeds and oilseeds are collectively referred to as cake feed. The by-products after oil extraction are collectively referred to as cake feed. The cake-like by-products after oil extraction by pressing are called oil cakes, and the fragment-like or coarse powder-like by-products after oil extraction by extraction are called oil cakes. [0003] Oil cake and oil meal are the main vegetable protein feeds in my country, which are widely used and used in large quantities. Common cakes include soybean cake, cottonseed cake, rapeseed cake, peanut cake, sunflower cake, flax cake, and a small amount of walnut cake, safflower cake, sesame cake, castor cake meal, palm meal, etc. [0004] The common characteris...

Claims

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Application Information

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IPC IPC(8): A23K1/14
Inventor 闫轶洁宋维平贠桂玲李曼韩科厅
Owner BEIJING DABEINONG TECH GRP CO LTD
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