Making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal
A corn germ meal, lactic acid bacteria fermentation technology, applied in the molding or processing of animal feed, animal feed, additional food elements, etc., can solve the problems of unbalanced amino acid composition, poor animal palatability, high crude fiber content, and increase feed intake. quantity and digestibility, harm reduction, and nutritional value enhancement
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Embodiment 1
[0023] With reference to the preparation method of the lactic acid bacteria of the present embodiment and the composite enzyme solid-state fermentation corn germ meal, it comprises the following concrete steps:
[0024] (1) Preparation of fermentation raw materials
[0025] The fermentation raw material - corn germ meal is crushed to 40 mesh (0.42mm) powder.
[0026] (2) Preparation of lactic acid bacteria liquid
[0027] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 36 hours, inoculate it on the MRS slant medium, inoculate it into the liquid medium after culturing on the slant surface for 36 hours, and culture it in shake flask for 12 hours, and the number of viable bacteria is 1.0×10 8 Inoculate the seed liquid into a 10...
Embodiment 2
[0033] With reference to the preparation method of the lactic acid bacteria of the present embodiment and the composite enzyme solid-state fermentation corn germ meal, it comprises the following concrete steps:
[0034] (1) Preparation of fermentation raw materials
[0035] The fermentation raw material - corn germ meal is crushed to 40 mesh (0.42mm) powder.
[0036] (2) Preparation of lactic acid bacteria liquid
[0037] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 42 hours, inoculate it on the MRS slant medium, inoculate it into the liquid medium after culturing it on the slant for 42 hours, and culture it in shake flask for 18 hours, and the number of viable bacteria is 3.0×10 8 Inoculate the seed liquid into a 100L se...
Embodiment 3
[0043] Referring to the preparation method of the lactic acid bacteria and compound enzyme solid-state fermentation corn germ meal of the present embodiment, it comprises the following specific steps:
[0044](1) Preparation of fermentation raw materials
[0045] The fermentation raw material - corn germ meal is crushed to 40 mesh (0.42mm) powder.
[0046] (2) Preparation of lactic acid bacteria liquid
[0047] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 48 hours, inoculate it on the MRS slant culture medium, inoculate it into the liquid medium after culturing it on the slant surface for 48 hours, and culture it in shake flask for 24 hours, and the number of viable bacteria is 2.0×10 8 Inoculate the seed liquid into a 10...
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