Fermented feed for cows and preparation method of fermented feed
A technology of fermented feed and fermented raw materials, applied in animal feed, animal feed, applications, etc., can solve the problems of restricting the widespread use of apple pomace, mycotoxin pollution, odor and blackening, etc., and achieve the improvement of feed resources that are increasingly scarce and environmentally friendly Pollution, the effect of optimizing milk quality
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Embodiment 1
[0035] A fermented feed for dairy cows and a preparation method thereof, the specific steps are as follows:
[0036] 1) Preparation of seed solution
[0037] Slant culture: under sterile conditions, Saccharomyces cerevisiae, Aspergillus niger and Bacillus subtilis were inoculated in the potato slant medium respectively, and cultured at a constant temperature of 30°C for 48 hours.
[0038] The composition of the potato slant medium is as follows: 150 g of potatoes, 10 g of glucose, 20 g of agar, 1000 ml of water, natural pH, and sterilized at 121° C. for 30 minutes.
[0039] Seed liquid culture: under aseptic conditions, wash the activated slant strains with physiological saline respectively, inoculate them into the seed medium, and cultivate them in constant temperature shake flasks at 30°C and 200r / min for 48 hours to obtain wine Yeast seed liquid, Aspergillus niger seed liquid and Bacillus subtilis seed liquid; the composition of the seed liquid culture medium: 250g of pota...
Embodiment 2
[0053] A fermented feed for dairy cows and a preparation method thereof, the specific steps are as follows:
[0054] 1) Preparation of seed solution
[0055] Slant culture: under sterile conditions, Saccharomyces cerevisiae, Aspergillus niger and Bacillus subtilis were inoculated in the potato slant medium respectively, and cultured at a constant temperature of 30°C for 48 hours. The composition of the potato slant medium is as follows: 150 g of potatoes, 10 g of glucose, 20 g of agar, 1000 ml of water, natural pH, and sterilized at 121° C. for 30 minutes.
[0056] Seed liquid culture: Under sterile conditions, wash the activated slant strains with physiological saline and inoculate them into the seed medium, and culture them in constant temperature shake flasks at 30°C and 200r / min for 48 hours to obtain Saccharomyces cerevisiae Seed solution, Aspergillus niger seed solution and Bacillus subtilis seed solution; the composition of the seed solution culture medium: 250g of pot...
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Abstract
Description
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Application Information
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