The invention discloses a simple
screening method for salt-tolerance maize inbred lines. A
germination period test and a
seedling stage test are both performed under the conditions of 0 mmol / L and 180 mmol / L. In the stress test of a
germination period, 15mL of
salt solution is replenished once each day, the number of germinated seeds of each inbred line is counted after the seeds are germinated for 4 days, and the
germination vigor of 16 inbred lines is calculated; the germination rate, the germinal length, the
root length, the germ
fresh weight and the root
fresh weight of the 16 maize inbred lines are measured after 7 days. In the
seedling stage test, 50mL of de-ionized water is replenished once every 2 days, 50mL of NaCl solution with corresponding concentration is replenished once every 3 days, and a second leaf is taken to measure the content of MDA, the
relative conductivity and the relative
water content after stress treatment is performed for about 10 days and a control leaf grows to three leaves with one center. According to the method, the salt injury index of each indicator is calculated by measuring a plurality of indexes in the germination period test and the
seedling stage test, the salt tolerance of each inbred line is subjected to
cluster analysis according to the salt tolerance index, and each inbred line is comprehensively evaluated to finally screen the salt-tolerance inbred lines. The simple
screening method is easy and convenient to operate and can be finished in a laboratory, the
workload is greatly reduced, the screening time is shortened, and the screening efficiency and the result accuracy are improved.