Simple screening method for salt-tolerance maize inbred lines

Abstract

The invention discloses a simple screening method for salt-tolerance maize inbred lines. A germination period test and a seedling stage test are both performed under the conditions of 0 mmol / L and 180 mmol / L. In the stress test of a germination period, 15mL of salt solution is replenished once each day, the number of germinated seeds of each inbred line is counted after the seeds are germinated for 4 days, and the germination vigor of 16 inbred lines is calculated; the germination rate, the germinal length, the root length, the germ fresh weight and the root fresh weight of the 16 maize inbred lines are measured after 7 days. In the seedling stage test, 50mL of de-ionized water is replenished once every 2 days, 50mL of NaCl solution with corresponding concentration is replenished once every 3 days, and a second leaf is taken to measure the content of MDA, the relative conductivity and the relative water content after stress treatment is performed for about 10 days and a control leaf grows to three leaves with one center. According to the method, the salt injury index of each indicator is calculated by measuring a plurality of indexes in the germination period test and the seedling stage test, the salt tolerance of each inbred line is subjected to cluster analysis according to the salt tolerance index, and each inbred line is comprehensively evaluated to finally screen the salt-tolerance inbred lines. The simple screening method is easy and convenient to operate and can be finished in a laboratory, the workload is greatly reduced, the screening time is shortened, and the screening efficiency and the result accuracy are improved.

Description

technical field [0001] The invention belongs to the technical field of breeding of new agricultural varieties, and in particular relates to a simple screening method for salt-tolerant corn inbred lines. Background technique [0002] The area of ​​saline soil in the world is very large. According to the statistics of only 103 countries, 203 million hectares 2 Of the irrigated area in China, there are 50 million hm 2 of saline soil. With the dual effects of population increase and arable land reduction, human beings are always facing food problems. Therefore, developing and utilizing saline-alkali land is an inevitable choice, and breeding salt-tolerant varieties is the best way. [0003] Maize is an important food and feed crop, moderately sensitive to salinity, and its salt tolerance is relatively poor. Whether the selected maize variety can be planted on saline soil requires scientific identification and evaluation of its salt tolerance, and the identification method an...

AI Technical Summary

Problems solved by technology

[0004] At present, most of the salt-tolerant germplasm screening of corn is to obtain salt-tolerant germplasm by comparing the relative yield reduction rate after field production. This method takes a long time, requires a certain amount of land, and is expensive.

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