Enzyme linked immunosorbent assay instrument testing method for penicillium marneffei mycelia-phase in-vitro susceptibility testing

Abstract

The invention discloses an enzyme linked immunosorbent assay instrument testing method for penicillium marneffei mycelia-phase in-vitro susceptibility testing. The light transmittance of a bacterium-containing diluent with an antibiotic medicinal liquid is tested by using an enzyme linked immunosorbent assay instrument in the penicillium marneffei mycelia-phase in-vitro susceptibility testing process. The densities are different in different bacterium growth situations, the light transmittances are also different, results are judged by using the enzyme linked immunosorbent assay instrument, apparent phenomena can be converted into values, and the operation can be relatively convenient and accurate; secondly, as an enzyme linked immunosorbent assay instrument pore plate is used, material consumption can be reduced; only a solution less than 1ml is needed in each pore, so that the amount of medicines is small, and waste can be greatly reduced; in addition, penicillium marneffei has infectivity, so that the pollution property can be further reduced because of the small use amount. Finally, normal growth of the penicillium marneffei can be not affected by the enzyme linked immunosorbent assay instrument, the operation is simple, convenient and rapid, and precise in-vitro susceptibility data results can be obtained.

Description

technical field [0001] The invention belongs to the technical field of Penicillium marneffei detection, and in particular relates to an enzyme-labeled detection method for an in vitro drug susceptibility test of Penicillium marneffei hyphae. Background technique [0002] Penicillium marneffei infection is the main infection factor leading to the death of AIDS patients in southern China, especially in Guangxi and Guangdong. Penicillium marneffei can cause deep fungal infection when human immunity is low, and invade multiple organs throughout the body. If it is not treated in time and correctly, it can lead to death. At present, the main therapeutic drugs for Penicillium marneffei are antibiotics, and amphotericin B (AmB), fluconazole, flucytosine, etc. are commonly used drugs for the treatment of Penicillium marneffei. [0003] Penicillium marneffei is the only pathogenic bacterium in Penicillium that exhibits a temperature biphasic type. It is in a mycelial state at a tempe...

AI Technical Summary

Problems solved by technology

The in vitro susceptibility test of Penicillium marneffei is mainly based on CLSI M38-A 2 However, it is difficult to judge the breaking point because it uses the visual method to judge the results, and it is difficult to obtain accurate results due to different personal judgments, which brings certain errors to the experimental results.

Shi Luyi, Zhang Hanyuan and others from the Wildlife Research Institute of Heilongjiang Province used a microplate reader to interpret the results based on the microdilution method of the in vitro drug sensitivity test, which greatly avoided the lack of readings, but the method was only used in the large intestine. Bacillus, other strains have not been reported

Zhou Zixiong and others from the School of Traditional Chinese Medicine of Guangdong College of Pharmacy also conducted research on the determination of the antibacterial activity of antibacterial substances by microplate reader and established a method, but the method was only applied to Staphylococcus aureus

Images