147 results about "Galactosidase alpha" patented technology

A method of enhancing the activity of lysosomal alpha-Galactosidase A (alpha-Gal A) in mammalian cells and for treatment of Fabry disease by administration of 1-deoxy-galactonojirimycin and related compounds.

The present invention provides a method treating a patient with Fabry disease by determining whether there is an improvement of a surrogate marker that is associated with Fabry disease following administration of a specific pharmacological chaperone of α-galactosidase A. The method includes administering an effective amount of 1-deoxygalactonojirimycn to the individual, wherein the 1-deoxygalactonojirimycin binds to alpha-galactosidase A in an amount effective to increase activity of the alpha-galactosidase A. The present invention also provides a method for monitoring and increasing a therapeutic response of a patient with Fabry disease following administration of a specific pharmacological chaperone of α-galactosidase A by evaluating the effect on the cytoplasmic staining pattern of a cell from the patient, wherein detection of a staining pattern in the cell that is similar to the staining pattern in a cell from a healthy individual indicates that the individual with Fabry disease is a responder.

A poultry feed composition including protein, vitamins and minerals, and a source of carbohydrates from the group consisting of soybean meals and corn supplemented with an alpha-galactosidase that catalyzes the degradation of the galactoside. The addition of the alpha-galactosidase increases the ratio of gain to feed, increases the amount of white meat, or decreases the amount of fat deposited during growth of a chicken fed the feed composition, relative to the chicken fed on an identical feed composition absent the alpha-galactosidase.

Fabry disease results from an X-linked deficiency in the enzyme α-galactosidase A. The present invention is directed to recombinant human α-galactosidase A and provides baculovirus expression vectors and recombinant virus that provide stable expression of extracellular and intracellular levels of this enzyme in an insect cell culture. The recombinant-derived enzyme can be used in enzyme replacement therapy to treat Fabry patients. Composition useful in therapeutic administration of α-galactosidase A are also provided.

The present application provides compositions comprising α-galactosidase A in combination with an active site-specific chaperone for the α-galactosidase A, and methods for treating Fabry disease in a subject in need thereof, that includes a method of administering to the subject such compositions. The present application also provides methods for increasing the in vitro and in vivo stability of an α-galactosidase A enzyme formulation. The present application also provides methods for treating Fabry disease using intravenous administration of 1-deoxygalactonojirimycin.

The present invention relates to methods and compositions for the reduction of xenotransplantation rejection. Specifically, the present invention relates, first, to transgenic cells, tissues, organs and animals containing transgenic nucleic acid molecules that direct the expression of gene products, including, but not limited to enzymes, capable of modifying, either directly or indirectly, cell surface carbohydrate epitopes such that the carbohydrate epitopes are no longer recognized by natural human antibodies or by the human cell-mediated immune response, thereby reducing the human immune system response elicited by the presence of such carbohydrate epitopes. In a preferred embodiment, the transgenic cells, tissues, organs and animals express nucleic acid molecules encoding functional recombinant alpha-Galactosidase A (alphaGalA) enzyme which modifies the carbohydrate epitope Galalpha(1,3)Gal. In a more preferred embodiment, the transgenic cells, tissues, organs and animals expressing the functional recombinant alphaGalA are transgenic pig cells, organs, tissues and / or animals. Second, the present invention relates to methods for xenotransplantation comprising introducing the transgenic cells, tissues and / or organs into human recipients so that a lower level of hyperacute rejection (HAR) is observed in the human recipients relative to the level of HAR observed in human recipients having received non-transgenic cells, tissues and / or organs.

An alpha-galactosidase gene, its encoded protein, production and use are disclosed. The process separating out alpha-galactosidase from (Penicillium sp.) F63 (CGMCC No. 1669) while purifying, re-separating, cloning to obtain gene for encoding the enzyme ( SEQ ID No: 1 or SEQ ID No: 2 nucleotide sequence ), constructing recombinant expression plasmid containing SEQ ID No: 1 nucleotide sequence, culturing host cell to obtain recombinant strain, inducing recombinant alpha-galactosidase expression, recovering, purifying and comparing to obtain final product. It can hydrolyze melitose and lupeose in bean. It can be used to feed and food industries.

The invention relates, in part, to improved methods of administering α-galactosidase A for the treatment of α-galactosidase A deficiencies including Fabry disease.

The invention provides a corn bean pulp type feed complex enzyme additive and an application thereof. The complex enzyme additive comprises the components based on weight percentage: 10%-15% of xylanase, 8%-15% of cellulase, 3%-8% of beta-seminase, 5-15% of alpha-galactosidase, 5%-10% of acid protease, 10%-20% of moderate temperature amylase, 5%-10% of glucoamylase and 7%-54% of carrier. The complex enzyme additive can decompose the nutrient s in the corn bean pulp, a mass of anti-nutritional factors which are hard to digest by animals such as the crude fiber and the like, so that energy in the corn bean pulp can be abundantly explored, the digestion capability of the animals to the corn bean pulp type feed can be improved, and the material-meat ratio can be reduced; the digestibility of the nutrient substance and the appearance metabolic energy can be improved, and the microorganism quantity of the ileum can be reduced; the corn bean pulp type feed complex enzyme additive has the advantages of being high-efficiency, specific, small in additive amount and the like compared with the similar additive; and after the complex enzyme additive is used, the growth of the animals can be promoted, and the feed converting efficiency can be improved.

The invention discloses a dura-mater biological patch and a preparation method thereof. The dura-mater biological patch adopts a process of using small intestinal submucosa tissues as materials and systemically removing immunogenic substances. The preparation method comprises the following steps of: using alcohol to treat and remove lipids, using trypsin and alkali solution to treat and remove cells, using DNA enzyme to treat and remove DNA, and using alpha-galactosidase to treat and remove alpha-Gal antigens and the like. The dura-mater biological patch and the preparation method disclosed by the invention have the advantages that not only can the immunogenic substances be effectively removed, but also the normal structure of extracellular matrix can not be damaged; and when the dura-mater biological patch is clinically applied in repairing dura-mater defects, the leakage of cerebrospinal fluid can be effectively prevented, the tissue can be guided for ingrowth, the tissue growing speed is matched with the patch decomposing speed, the immunological rejection is low and the biocompatibility is good.

This invention relates to enzymatic removal of type A and B antigens from blood group A, B, and AB reactive cells in blood products, and thereby converting these to non-A and non-B reactive cells. The invention further relates to using unique alpha N-acetylgalactosaminidases and alpha-galactosidases with superior kinetic properties for removing the immunodominant monosaccharides of the blood group A and B antigens and improved performance in enzymatic conversion of red blood cells. The preferred unique alpha-N-acetylgalactosaminidases and alpha-galactosidases exhibit the following characteristics: (i) exclusive, preferred or no less than 10% substrate specificity for the type A and B branched polysaccharide structures relative to measurable activity with simple mono- and disaccharide structures and aglycon derivatives hereof; (ii) optimal performance at neutral pH with blood group oligosaccharides and in enzymatic conversion of cells; and (iii) a favorable kinetic constant Km with mono- and oligosaccharide substrates. The conversion methods of the invention use significantly lower amounts of recombinant glycosidase enzymes than previous and result in complete sero-conversion of all blood group A and B red cells.

Multimeric protein structures comprising at least two alpha-galactosidase monomers being covalently linked to one another via a linking moiety are disclosed herein, as well a process for preparing same, and methods of treating Fabry disease via administration of a multimeric protein structure. The disclosed multimeric protein structures exhibit an improved performance, in terms of enhanced activity and / or a longer lasting activity under both lysosomal conditions and in a serum environment.

Nucleic acid expression constructs are provided and, more particularly, nucleic acid constructs for expression of human alpha-galactosidase in plant cells, cells expressing the nucleic acid construct, producing the human alpha-galactosidase and uses thereof.

The invention discloses a method for hydrolyzing vegetable protein and belongs to the field of feed processing. The method for hydrolyzing vegetable protein disclosed by the invention sequentially comprises the following steps: adding a mixture of enzymes including alpha-amylase, cellulose, hemicellulase, pectinase, alpha-galactosidase and phytase at a mass ratio of 10:5:5:2:2:1 into a vegetable protein raw material, and hydrolyzing for 2-5 hours, wherein the addition amount of the mixture is 3-8 g / Kg of the vegetable protein raw material; adding neutral protease, and hydrolyzing for 2-5 hours, wherein the addition amount of the neutral protease is 2-4 g / kg of the vegetable protein raw material; adding alkaline protease, and hydrolyzing for 2-5 hours, wherein the addition amount of the alkaline protease is 3-5 g / kg of the vegetable protein raw material; drying to obtain powdery vegetable hydrated protein. The method for hydrolyzing vegetable protein disclosed by the invention is capable of hydrolyzing a plurality of plant protein raw materials and reducing nutrient levels in the plant raw materials and is thorough in the hydrolyzing process.

The invention discloses a complex enzyme preparation improving broiler chicken breeding performance. The complex enzyme preparation is characterized by being prepared from a carrier, xylanase, cellulase, alpha-galactosidase, beta-mannase, pectinase, amylase, lipase and protease, wherein xylanase, cellulase, alpha-galactosidase, beta-mannase, pectinase, amylase, lipase and protease are added in the carrier. In each gram of the complex enzyme preparation improving broiler chicken breeding performance, the activity of xylanase is 1000-10000 U, the activity of alpha-galactosidase is 2-40 U, the activity of cellulase is 100-3000 U, the activity of beta-mannase is 100-2500 U, the activity of pectinase is 100-3000 U, the activity of amylase is 100-3000 U, the activity of lipase is 50-2000 U, and the activity of protease is 1000-10000 U. According to application of the complex enzyme preparation improving broiler chicken breeding performance in broiler chicken feed, 100-1000 g of the complex enzyme preparation is added in each ton of complete feed. The complex enzyme preparation can generate the emergy of 25-85 kcal / kg, effectively degrade antinutritional factors such as soluble xylan, mannan and alpha-galactoside, increase the digestion rate of nutrients such as protein and amino acid, improve broiler chicken productivity and lower breeding cost.

The invention relates to a high-quality enzymolysis-fermented bean pulp as well as a preparation method and application thereof. The preparation method comprises the steps of crushing peeled bean pulp, sieving by virtue of an 80-mesh sieve, carrying out primary enzymolysis by virtue of alpha-galactosidase and glucoamylase, carrying out secondary enzymolysis by virtue of papain, carrying out enzyme deactivation, inoculating a compound strain of saccharomycetes, bacillus and lactic acid bacteria, sequentially carrying out aerobic fermentation and anaerobic fermentation to obtain a finished product, and carrying out spray drying on the finished product, so as to preserve active components to the greatest extent. The prepared enzymolysis-fermented bean pulp has the special fermentation flavor, the protein content is about 55%, the acid soluble protein content is more than 50%, antinutritional factors such as antigens are fundamentally eliminated, the components are stable, and the enzymolysis-fermented bean pulp has the characteristics of strong hydrophily, high water holding ratio and strong palatability and has an obviously food calling effect on aquatic animals; and the enzymolysis-fermented bean pulp is rich in functional small peptides, probiotics and metabolite of the probiotics, beneficial to the nutrient balance of intestinal tract of bred animals and is suitable for being added into feeds of piglets, poultries, pets, aquatic products and the like, the problem of trophic diarherra of the animals is solved, and the suggested use ratio is 2%-5%.

A method is described for improving the digestibility of a forage diet for ruminant animals. A forage, including alfalfa, Chinese wildrye, corn silage, straw silage, corn stover, ryegrass or TMR, is treated with an enzyme product having cellulase, xylanase, beta-glucanase, pectinase, mannanase and alpha-galactosidase activities.

The invention discloses high-protein fermented soybean meal and a preparation method of the high-protein fermented soybean meal, and belongs to the field of animal feeds. The preparation method of the high-protein fermented soybean meal comprises the following steps: grinding soybean meal raw materials, adding water to carry out sterilization, adding a compound enzyme preparation to carry out enzymatic hydrolysis, after the enzymatic hydrolysis is carried out for a period of time, adding activated lactic acid bacteria or yeast simultaneously, performing semi-solid state fermentation, and then solid-liquid separation, drying and grinding to obtain the high-protein fermented soybean meal; the compound enzyme preparation contains alpha-galactosidase, cellulase, xylanase, pectinase, beta-mannase and beta-glucanase. According to the invention, through the specific composition and addition amount of the compound enzyme preparation, the synergism among various enzymes and a method of combining adding of the compound enzyme preparation with mixed fermentation, the content of non-starch polysaccharides can be reduced to 8% to 10%, the protein content reaches up to 60.2%, essential amino acids are overall improved by 22.41%, other various indexes obviously become better, and the method disclosed by the invention plays an important role in improving the quality of the soybean meal.

The present invention relates to a palm bio-feed additive, a preparation method thereof and a bio-feed containing the additive. The palm bio-feed additive comprises the following components in parts by weight: 985-995 parts of palm meal and 5-15 parts of digestive auxiliary materials. The digestive auxiliary materials comprise probiotics and compound enzymes at a mass ratio of 1:1. The probioticscomprise the following micro-organisms in parts by weight: 1-3 parts of bacillus subtilis powder, 1-3 parts of bacillus licheniformis powder and 0.5-1.5 parts of lactic acid bacterium powder. The compound enzymes comprise the following enzymes in parts by weight: 1-3 parts of xylanase, 1.5-4.5 parts of beta-mannanase, 0-1 part of acidic protease, 0-1 part of cellulase, 0-0.5 part of beta-glucanase, 0-0.5 part of medium-temperature alpha-amylase, 0-0.3 part of pectinase, 0-0.7 part of neutral protease, 0-0.5 part of alkaline protease and 0-0.5 part of alpha-galactosidase. The palm bio-feed additive prepared by using the components in the above ratios has characteristics of improving digestion and absorption of livestock and poultry, and being small in side effects and high in utilization rate of amino acids.

The invention provides a special complex enzyme preparation for wheat for livestock and poultry and application thereof. The special complex enzyme preparation comprises xylanase, beta-glucanase, beta-mannase, cellulase, acid protease, neutral protease, pectinase, alpha-galactosidase, glucoamylase and aspergillus niger fermentation complex enzyme; and an antioxidant, a mildew-proof agent, an insect repellant and a carrier are added. According to the invention, the special complex enzyme for wheat is prepared from xylanase for effectively reducing viscosity and mono-enzymes such as beta-glucanase, beta-mannase, cellulase, acid protease and the like; and the preparation is simpler than the operation of preparing a complex enzyme by fermenting aspergillus niger only, has low labor capacity and cost, and realizes a good enzymolysis and viscosity reduction effect. Through the invention, the anti-nutrition effect caused by adding high-proportion wheat in daily ration can be effectively overcome, the chyme viscosity is reduced, the production performance of animals is enhanced, the utilization of wheat and wheat enzyme is optimized for a feed enterprise, and the feed cost is effectively lowered.

The invention provides a novel complex enzyme product special for ruminants and belongs to the technical field of enzymic preparations. The novel complex enzyme product comprises 1,500-2,500 U / g xylanase, 3,000-4,000 U / g cellulase, 15,000-20,000 U / g beta-glucanase, 20-80 U / g alpha-galactosidase, 35,000-40,000 U / g alpha-amylase, 3,000-4,000 U / g acid proteinase and 3,000-4,000 U / g papain. According to the novel complex enzyme product special for the ruminants, rumen enzyme activity can be improved, the rumen fermentation type can be improved, the digestion rate of coarse materials and fine materials can be increased, the production performance of the ruminants can be improved, the price is low, and the varieties of enzyme products special for the ruminants are enriched.

The invention relates to an Alkali-resistant low-temperature alpha-galactosidase AgaAJB13 and genes thereof. The invention provides an alpha-galactosidase AgaAJB13 derived from Sphingobium sp., a coding gene agaAJB13 for encoding the alpha-galactosidase AgaAJB13, a recombinant vector containing the gene agaAJB13 and a recombination strain containing the gene agaAJB13, wherein, the amino acid sequence of the alpha-galactosidase AgaAJB13 is shown in SEQ ID No.1. The alpha-galactosidase AgaAJB13 has the following properties that: the optimal PH is 5.0; the optimal temperature is 60 DEG C, the enzyme activity of more than 10% and the enzyme activity of more than 20% can be achieved respectively at the temperature of 10 DEG C and 20 DEG C; the activity of about 40% can be maintained after processing for 1h at the temperature of 37 DEG C by using a buffer with 0.1M and pH 11.0; and the alpha-galactosidase AgaAJB13has good thermal stability, good proteinase resistance and good hydrolysis to various natural supports, thus being capable of being used as a feed or food additive in fields of feed and food.

The invention discloses a feeding complex enzyme preparation by using Chinese medicaments as carriers and a diluting agents, which consists of xylanase powder, cellulose powder, phytase powder, lipase powder, alpha-galactosidase powder, beta-glucanase powder, mannose powder, acid proteinase powder, pectinase powder, astragalus powder and eucommia. Each enzyme powder is cultured and fermented by single enzyme strain, and adsorbed by taking the astragalus powder and the eucommia powder as carriers, and mixed with other single enzyme powders to prepare the feeding complex enzyme preparation. When reinforcing related nutrients or degrading antinutritional factors in the feed, the complex enzyme can be used for promoting dissolution of effective components in the Chinese medicaments, so that the absorption rate of feed and nutrients in the Chinese medicaments by pork pigs can be improved, and the quality of pork quality can be improved.

An Aspergillus niger variant (CGMCC No.1182) of the high-activity alpha-galactosidase is disclosed. It is suitable to grow in Cha's culture medium at 25 deg.C for 7 days and its spore shows tawny. It can be used to produce the high-activity alpha-galactosidase by solid fermenting. Its average enzyme activity can reach 286-345 IU / g, increasing it by 2.26 times.

The invention relates to the field of genetic engineering and particularly provides neutral alpha-galactosidase Aga-S27 having high degradability of alpha-galactoside oligosaccharide, and genes and application thereof. The neutral alpha-galactosidase Aga-S27 having high degradability of alpha-galactoside oligosaccharide can be obtained from a bacterial strain, i.e., novel streptomyces sp.S27 (CGMCC No.3146) screened by the invention, and the genes thereof and recombinant vector containing the genes can be further obtained, wherein the amino acid sequences of alpha-galactosidase Aga-S27 are shown in SEQ ID No.1. The alpha-galactosidase Aga-S27 has the advantages of appropriate operating temperature and pH value, higher protease resistance and higher ability to hydrolyze various substrates. Therefore, the alpha-galactosidase Aga-S27 is applicable as feed or food additives in the industries of feed and food.

The invention discloses a preparation method and application of a Trichoderma reesei strain capable of highly yielding penicillium janczewskii Zaleski alpha-galactosidase. The Trichoderma reesei strain capable of highly yielding the penicillium janczewskii Zaleski alpha-galactosidase is obtained by introducing an encoding gene of the penicillium janczewskii Zaleski alpha-galactosidase and a selection marker gene to a host, namely Trichoderma reesei. The efficient secretory expression of the penicillium janczewskii Zaleski alpha-galactosidase is realized in Trichoderma reesei by utilizing a strong promoter of Trichoderma reesei cbh1 and a signal peptide sequence of the strong promoter. Experiments prove that the enzyme activity of a fermentation solution obtained through fermenting the Trichoderma reesei strain capable of highly yielding the penicillium janczewskii Zaleski alpha-galactosidase can be up to 119.16U / ml; and the fermentation process is simple, the raw materials are cheap and available, the production cost is greatly reduced, and the preparation method is very suitable for industrial production and application.

The invention relates to bio-enzyme gel breaker and applications thereof. The bio-enzyme gel breaker is an aqueous solution of beta-mannanase, alpha-galactosidase, and a cross-linking agent. According to an application method, by volume, 80 to 120 parts of a fracturing fluid base solution is mixed with 5 to 10 parts of the bio-enzyme gel breaker, and after gel forming, an obtained mixture is taken as a fracturing fluid. According to applications, galactosidase is used for removing a part of galactose residues on the side chains of galactomannan, so that mannose glycosidic bonds on the main chain of galactomannans are exposed, and then degradation effect of mannanase is adopted for selective degradation of galactomannan. Combination of the above two enzymes is capable of realizing high-efficiency rapid gel breaking of the fracturing fluid, reducing molecular weight of gel breaking products greatly, and reducing reservoir damage.

The invention relates to a preparation method of chitosan nano particles embedded with alpha-galactosidase in the technical field of feeding enzymic preparations, which comprises the steps: preparingthe chitosan stock solution; adding alpha-galactosidase crude enzyme solution with the same volume into the chitosan stock solution, and evenly mixing by adopting the magnetic stirring method to prepare alpha-galactosidase mixed solution; adding sodium tripolyphosphate water solution drop by drop into the alpha-galactosidase mixed solution, and magnetic stirring at room temperature to prepare thechitosan nano particles embedded with alpha-galactosidase. The invention has simple preparation operation and can obtain chitosan nano particles embedded with alpha-galactosidase by the simple mixingof chitosan / alpha-galactosidase / sodium tripolyphosphate; the particles are not adhered, have good ballability and round surfaces, can release enzymic preparations fast at the acid or alkaline condition consistent with the acidity and alkalinity of the stomach and intestines of the pig, and is beneficial to the better efficacy exertion of the feeding alpha-galactosidase.