34results about How to "High feeding concentration" patented technology

The invention relates to a method for hydroxylating dehydroisoandrosterone (DHEA) by using colletotrichumlini ST-1. The method for hydroxylating the dehydroisoandrosterone by using the colletotrichumlini ST-1 is characterized in that: in a conversion process, a way of pre-including the DHEA and methyl-beta-cyclodextrin with an equimolar ratio first and then feeding is adopted, so that the water solubility and the conversion efficiency of the DHEA are significantly improved. Under a condition that a substrate feeding amount is 10g / L, the conversion rate is as high as 95% and the total yield of products 7 alpha-OH-dehydroepiandrosterone and 7,15 alpha-OH-dehydroepiandrosterone is 80.94%.

The present invention discloses the preparation process of slowly releasing oil soluble medicine microballoon. The water solution of PVA, the water solution of PEG or distilled water as water phase; PLA-PEG copolymer as oil phase; and acetone solution of medicine are mixed via stirring to add oil phase into water phase and volatilized to eliminate acetone, and through further dialysis in dialyzing bag, un-enveloped medicine is eliminated. The present invention has microballoon size controllable below 150 nm and certain slow releasing property.

The invention discloses synthesis of 2-N-methylaminomethyl-5-N-methylaminomethyl furan, and relates to the field of synthesis method of organic compounds. According to the synthesis method, 5-hydroxymethyl furfural, organic amine, and a formic acid water solution are mixed in a reactor; the mixture is heated to carry out reactions, after reactions, rotation evaporation is performed to remove the solvent to obtain a solution A; adding a NaOH solution into the solution A, adjusting the pH of the solution until pH is not less than 10 to obtain a solution B; extracting the solution B by ethyl acetate, merging extracting agents, carrying out reduced pressure distillation to recover the extracting agent namely ethyl acetate to obtain a solution C; and subjecting the solution C to reduced pressure distillation to obtain 2-N-methylaminomethyl-5-N-methylaminomethyl furan. The purity of obtained 2-N-methylaminomethyl-5-N-methylaminomethyl furan is more than 99%. HMF and organic amine are taken as the raw materials, a formic acid water solution is taken as the reaction solution, and 2-N-methylaminomethyl-5-N-methylaminomethyl furan is prepared by a one-pot method. The operation is simple, thereaction system is green and environmentally friendly, the reaction solvent can be repeatedly used, the feed concentration is large, and the yield is high.

The invention belongs to the technical field of biology, and particularly relates to a 5L fermentation tank technique for efficiently converting DHEA (dehydroepiandrosterone) by using Colletotrichum lini ST-1. The technical scheme is as follows: the method comprises the steps of strain activation, seed culture, DHEA bioconversion, product detection and the like. Substrate consumption, residual sugar content and other indexes in the conversion process are monitored, and the batch-by-batch substrate addition is combined with reducing force regeneration promotion to solve the problems of lower substrate feed concentration and lower conversion efficiency. The method increases the product yield, shortens the conversion period, is simple to operate, has high feed amount, and is suitable for large-scale production of steroid compound bioconversion.

The invention discloses a preparation method of high-quality prednisone acetate and an intermediate thereof. The method includes the steps of: taking epihydrocortisone as the raw material, firstly conducting microbial dehydrogenation to obtain 11alpha, 17alpha, 21-trihydroxy-pregn-1, 4-diene-3, 20-dione intermediate, then carrying out esterification reaction to obtain 11alpha, 17alpha, 21-trihydroxy-pregn-1, 4-diene-3, 20-dione-21-acetate, and finally carrying out oxidation reaction to obtain prednisone acetate. The method provided by the invention solves the technical difficulty of non-idealintroduction of C1, 2 double bond in the traditional fermentation production process of prednisone acetate. The cortisone acetate prepared by the method provided by the invention has high quality andextremely low impurity content, greatly improves the multidirectional application of prednisone acetate, and meanwhile, the process route has the characteristics of low cost and simple operation.

The invention discloses ascorbate glucoside crystalline powder and a preparation method thereof, and belongs to the field of biochemical engineering. According to the ascorbate glucoside crystalline powder, by improving the transformation concentration of ascorbate glucoside crystalline, impurities and pigments in a transformation system are removed through a multi-stage membrane separation technology, and the ascorbate glucoside crystalline is separated from ascorbate to further concentrate and recycle the ascorbate; mother liquor is filtered under an acidic environment, and the light transmittance of a product is improved; meanwhile, the problem of browning caused by easy oxidization of the ascorbate glucoside crystalline in the drying process is solved by adopting a vacuum microwave drying technology.

The invention discloses penicillium simplicissimum WY134-2 and a method for producing 1,2-testololactone by converting C21 or C19 steroids through fermenting penicillium simplicissimum. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on August 12, 2013 and has the collection number of CGMCC No. 8017. The production method comprises the following steps: by adopting penicillium simplicissimum WY134-2 as a strain, carrying out primary seed culture and secondary fermentation enlargement culture, putting the crushed steroids into a thallus fermentation broth, acidifying, extracting with an organic solvent, and separating by virtue of silica gel column chromatography to obtain the product, namely, 1,2-testololactone of which the yield is 56-96%. The method is simple and convenient and has the advantages of mild conditions, less byproducts and no environmental pollution.

The invention belongs to the technical field of microorganisms and relates to an application of a nano-liposome technology in bioconversion. The application adopts lecithin as a carrier to prepare hydrophobic substrate into nano lipsome, and then carries out microbial conversion. The application is especially applicable to microbial conversion of a hydrophobic compound, or microbial conversion of the substrate which has toxicity to the microbe and inhibiting the growth of the microbe. The application has the advantages that by utilization of the characteristic of the lecithin for increasing the permeability of a cell membrane, the advantages of good biocompatibility of nano liposome and improvement on the water solubility and large special surface area can be exerted, the transportation of the substrate into cells is enhanced, and the amount of the substrate entering the microbial cells, so that the feeding concentration and the conversion rate of the substrate in a conversion system are increased. The invention provides a new method for microbial conversion of the hydrophobic compound.

The invention relates to a preparation method of chitosan nano particles embedded with alpha-galactosidase in the technical field of feeding enzymic preparations, which comprises the steps: preparing the chitosan stock solution; adding alpha-galactosidase crude enzyme solution with the same volume into the chitosan stock solution, and evenly mixing by adopting the magnetic stirring method to prepare alpha-galactosidase mixed solution; adding sodium tripolyphosphate water solution drop by drop into the alpha-galactosidase mixed solution, and magnetic stirring at room temperature to prepare thechitosan nano particles embedded with alpha-galactosidase. The invention has simple preparation operation and can obtain chitosan nano particles embedded with alpha-galactosidase by the simple mixingof chitosan / alpha-galactosidase / sodium tripolyphosphate; the particles are not adhered, have good ballability and round surfaces, can release enzymic preparations fast at the acid or alkaline condition consistent with the acidity and alkalinity of the stomach and intestines of the pig, and is beneficial to the better efficacy exertion of the feeding alpha-galactosidase.

A process of preparing phenolic ketone by way of micro-conversion. Strain is simple bacillus. Substrate is steroids (1 and 2). The process contains preparation of suspension, culture of seed solution, after-fermentation, substrate conversion and separation of product. Inventory rating is 12-20g / L fermenting solution. Level of O2 is between 30% and 50%. Time is between 30 and 60h. Adopt acetone-water method so that the product agrees USP26.

The invention provides a method for preparing a steroid drug intermediate employing bioconversion phytosterol. The method comprises the following steps: 1) providing turbid liquid of phytosterol; 2) providing a resting cell of a microbial strain for converting the phytosterol into a steroid drug intermediate; 3) mixing the turbid liquid of the phytosterol with the resting cell, and adding beta-cyclodextrin or chemically modified beta-cyclodextrin to evenly mix, and cultivating to finish conversion; 4) centrifuging the conversion liquid obtained in the step 3), separating supernatant from thallus, separating to obtain the steroid drug intermediate from the supernatant by solvent extraction. By adopting the method provided by the invention, the sterol feeding concentration is improved, the target product quantity is increased, simple separation of the thallus, a cosolvent and a product is achieved when the conversion lasting time is shortened, repeated use of the thallus and the cosolvent for a plurality of times is achieved, the production cost is greatly saved, and the method is applicable to industrial production.

This invention relates to a method of using gas containing molecular oxygen to catalytic oxidate double-gan phosphine for preparing glyphosate. The steps:(1) mix double-gan phosphine, water and activated carbon, at condition of speed of agitator 800 to 1500 r / m , inlet oxygen enrichment gas to carry out reaction, reactant liquor for refrigeration, glyphosate seed out, collect glyphosate and activated carbon blending press cake, divide mother solution with deleterious impurities such as formaldehyde; (2) plunge glyphosate and activated carbon admixture press cake to glyphosate saturation solution, after glyphosate dissolving take filtration while hot, filtrate recooling, glyphosate seedout, cake drying, then gain glyphosate solid; or plunge press cake to water, cooling, drop isopropylamine or inlet alkaline air, directly prepare isopropylamine salt or ammonium salt water solution.

The invention discloses a novel 12-hydroxycholic acid dehydrogenase derived from Eggerthella lenta and its encoding gene, and uses the enzyme as a biocatalyst to catalyze the oxidation of cholic acid and its derivative 12-hydroxyl to a carbonyl substrate. The gene encoding this enzyme has low homology with the currently known 12-hydroxysteroid dehydrogenase, and has high enzyme activity to 12-hydroxycholic acid and its derivatives, and in the process of cultivating the Escherichia coli expressing the gene, no Antibiotics are required, and efficient expression can be achieved without traditional isopropyl‑β‑D‑thiogalactopyranoside (IPTG) induction. In the conversion process, after concentrating the bacteria, the substrate concentration can reach 200 g / L, and the conversion rate is >95%, and no organic solvent is used in the reaction process, and the conversion method is green and environmentally friendly.

The invention discloses synthesis of 2-N-methylaminomethyl-5-N-methylaminomethyl furan, and relates to the field of synthesis method of organic compounds. According to the synthesis method, 5-hydroxymethyl furfural, organic amine, and a formic acid water solution are mixed in a reactor; the mixture is heated to carry out reactions, after reactions, rotation evaporation is performed to remove the solvent to obtain a solution A; adding a NaOH solution into the solution A, adjusting the pH of the solution until pH is not less than 10 to obtain a solution B; extracting the solution B by ethyl acetate, merging extracting agents, carrying out reduced pressure distillation to recover the extracting agent namely ethyl acetate to obtain a solution C; and subjecting the solution C to reduced pressure distillation to obtain 2-N-methylaminomethyl-5-N-methylaminomethyl furan. The purity of obtained 2-N-methylaminomethyl-5-N-methylaminomethyl furan is more than 99%. HMF and organic amine are taken as the raw materials, a formic acid water solution is taken as the reaction solution, and 2-N-methylaminomethyl-5-N-methylaminomethyl furan is prepared by a one-pot method. The operation is simple, thereaction system is green and environmentally friendly, the reaction solvent can be repeatedly used, the feed concentration is large, and the yield is high.

The invention discloses a method for preparing 11α, 17α-hydroxyprogesterone by conversion of immobilized hydroxylase, which is characterized in that 11α, 17α-hydroxyprogesterone is prepared by conversion of immobilized hydroxylase with 17α-hydroxyprogesterone as a substrate Hydroxyprogesterone includes preparation of medium, cultivating Aspergillus ochrae in the medium, induction of hydroxylase, preparation of immobilized hydroxylase, preparation of transformation system, conversion of substrate, extraction and purification of products and other steps. The invention has the advantages of high feeding concentration, high conversion rate of substrate, short conversion cycle, easy separation of products and repeated use of immobilized hydroxylase. The feeding concentration of 17α-hydroxyprogesterone in a single enzyme reaction is 40g / L, and the molar conversion rate can reach 93%. At the same time, the immobilized hydroxylase can be used repeatedly for more than 3 times. The invention has simple operation process, low production cost and high industrial application prospect.

The invention relates to a biotransformation preparation method of a steroid intermediate, belonging to the technical field of biopharmaceuticals. The present invention first prepares the steroidal suspension of intermediate I, then puts the suspension into the fermentation medium containing mold seeds, and C occurs under the action of mold 11 Hydroxylation and hydrolysis of C at the same time 21 Acetate, to obtain intermediate II. The invention adopts the emulsification feeding method to replace the operation of organic solvent dissolving substrate feeding, the concentration of feeding substrate can be increased to more than 15g / L, and the biotransformation rate can be increased to more than 90%. By adopting the method, adding nitrogen source nutrients while emulsifying, the biotransformation is more thorough under the condition of increasing the substrate feeding concentration, and the production cost is significantly reduced.

The invention discloses a strain of Penicillium simplicissimum WY134-2 and a method for producing 1,2-dihydrotestolactone (testololactone) by fermenting and transforming C21 or C19 steroid compounds with the strain. The strain was deposited on August 12, 2013 in the General Microbiology Center of the China Committee for the Collection of Microorganisms, with a preservation number of CGMCC NO.8017. The production method is as follows: using Penicillium simplicissimum WY134‑2 as the bacterial species, through primary seed cultivation and secondary fermentation expansion cultivation, putting pulverized steroid compounds into the bacterial fermentation broth, acidifying, and extracting with organic solvents , and silica gel column chromatography to obtain the product 1,2-dihydrotestolactone with a yield of 56-96%. The method is simple and convenient, has mild conditions, few by-products, and does not pollute the environment.

This invention relates to a new Rhodotorula.sp used to prepare DCPC by biotransformation method and the method of using this strain to prepare DCPC. One kind of microorganism strain, its features is that the strain is rhodotorula category, its name is Rhodotorula R-92, its number in preservation center is CGMCC NO.1277, strain is used as biotransformation agent in this transform method. Organism transformation substrate is cephalosporin C salt water solution. Transformation agent is added to substrate to transform or blending ferment, then white or tiny yellow DCPC powder is got after resin proposal, concentration and solvent crystallization. The goal of this invention is to solve the problem of low yield and high cost and not fit to industrial scale generating in the generation of DCPC.