901 results about "Bioconversion" patented technology

A new polypropylene terephthalate composition is provided. The polypropylene terephthalate is comprised of 1,3-propanediol and terephthalate. The 1,3-propanediol is produced by the bioconversion of a fermentatble carbon source, preferable glucose. The resulting polypropylene terephthalate is distinguished from petrochemically produced polymer on the basis of dual carbon-isotopic fingerprinting which indicates both the source and the age of the carbon.

Methods for solubilizing algae or algal material are provided to facilitate the recovery of oil or lipids, as well as hydrocarbons and carbohydrates, from algae or algal material. The methods involve contacting algae or algal material with an oxoacid ester or thioacid ester of phosphorus or a mixture of an oxoacid of phosphorus and / or an alcohol to form a mixture thereof under conditions effective to solubilize the algae or algal material. These methods optionally further comprise bioconversion of the solubilized algae or algal material to form a composition suitable for recovery of oils and non-oil chemicals.

PCT No. PCT / IL98 / 00437 Sec. 371 Date Feb. 8, 2000 Sec. 102(e) Date Feb. 8, 2000 PCT Filed Sep. 10, 1998 PCT Pub. No. WO99 / 12429 PCT Pub. Date Mar. 18, 1999A method of bioconversion of organic industrial or agricultural cellulose containing wastes into proteinaceous product. The method comprises comminution of the wastes with moistening and addition of a starting culture inducing their biological degradation and conversion into simple carbohydrates. The carbohydrates are fermented into digestible products. The starting culture comprises cleaving enzymes produced by edible microorganisms such as fungus and bacteria selected from the group consisting of Ilumicola grisea, Trichoderma harzanum, Ruminococcus albus.

A method of producing fuel from biodegradable carbonaceous material using a stacked particle bioreactor is provided. A stacked particle bioreactor is formed from particles including biodegradable carbonaceous material. The biodegradable carbonaceous material in the stacked particle bioreactor is aerobically and / or anaerobically bioconverted into one or more synfuels, which are collected from the reactor. The synfuels produced by the method may include synthetic petroleum, alcohol, and / or a gaseous fuel containing methane. Preferably the method includes an aerobic biotreatment phase followed by an anaerobic bioconversion phase. A stacked particle bioreactor for carrying out the anaerobic, and preferably aerobic, degradation is also described.

A method for the evaluation, design and optimization of in-situ bioconversion processes for the conversion of carbon to methane and other useful gases and liquids. The method utilizes a comprehensive computer simulation model for accurately simulating the physical and dynamic conditions in a subterranean carbon-bearing formation and the effects of stimulating the growth of indigenous or non-indigenous microbes therein for the bioconverstion of carbon to methane and other useful gases and liquids. The method enables the prediction of bioconversion rates and efficiencies under a range of variables, and thus provides for the optimization of in-situ bioconversion process design and operation.

A method, system, apparatus and program extracts energy from organic residual materials produced by the manufacturing of biofuels. Energy is extracted from the biofuels residuals using anaerobic bioconversion to produce a fuel for use in the manufacturing process for producing synthetic biofuel or as an additional energy product for sale comprises: providing at least one bioconversion tank for conversion of organic waste material, the bioconversion tank containing an active biomass comprising at least one bacteria that decomposes organic material; providing at least one inlet to the bioconversion for organic material; a processor that receives and stores information on: the status of chemical oxygen demand of the active biomass; and the oxygen provision capability of any organic material that can be fed into the bioconversion tank through an inlet; a mass flow control system controlled by the processor which feeds at least one organic material through an inlet at a rate based at least in part upon the status of chemical oxygen demand in the bioconversion tank as recognized by the processor.

The present invention provides means for the production of desired end-products of in vitro and / or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and / or liquefaction of the substrate.

The invented process separates main components in lignocellulosic biomass, specifically hardwoods, softwoods into lignin and fractions of high purity sugars which are used for ethanol production. The invented process comprises of treatment stages at high temperature and high pressure with hydrochloric acid or sulfuric acid. Residual lignin and extractives in the cellulosic solid fraction are selectively removed by chemical treatments of sodium chlorite, anhydrous acetic acid, chlorine and chlorine dioxide to enhance the purity and biological conversion of cellulose to ethanol. The pre-hydrolysate generated from the acid treatment stage, containing xylose, arabinose, galactose, glucose and the purified cellulosic fraction are enzymatically hydrolyzed and fermented to produce ethanol. Significant amount of lignin from the process is recovered as a by-product.

A method for processing organic material, in which method bioconversion is performed on the organic material in at least one first reactor, the biogas formed in the bioconversion is treated with ammonia in at least one second reactor and buffer solution produced in the second reactor is recycled to the bioconversion in the first reactor. Thus, the carbon dioxide of the mixed methane / carbon dioxide gas reacts with the ammonia and forms a buffer compound, such as ammonium bicarbonate and / or ammonium carbonate.

The invention discloses a Pleurotus eryngii culture medium and a manufacturing method thereof. The culture medium comprises 75 to 80 percent of cottonseed hulls or wheat straws or straws or corncob, 5 to 12 percent of wheat bran or rice husks, 8 to 10 percent of dry fowl manure, 3 to 8 percent of cornmeal, 1 to 2 percent of hemihydrate gypsum, 0.5 to 1.5 percent of sucrose and 0.5 to 1.5 percent of quicklime, and is added with 0.1 to 0.3 percent of potassium dihydrogen phosphate and 0.1 to 0.2 percent of magnesium sulfate according to the total amount of the compositions, and various compositions are calculated by weight percentage. The cottonseed hulls or the wheat straws or the straws or the corncob and the dry fowl manure are stacked and retted before use; and the culture medium after cultivation of Pleurotus eryngii is subjected to on-the-spot returning treatment. By adoption of the technical proposal, the method can make branched oyster mushrooms organisms well grown, overcome the defect that the prior art has no culture medium specially prepared for branched oyster mushrooms, simplify the production process, shorten the growth period of the Pleurotus eryngii, and improve the bioconversion of the Pleurotus eryngii.

Sterile nanoemulsions are prepared containing oily droplets of a diameter of less than 100 nm in an aqueous phase. The oily droplets contain a lipophilic substance, and have on their surface an amphoteric emulsifier in an amount of preferably 0.65 to 0.75 parts by weight amphoteric emulsifier for one part by weight of oily component forming the droplets. The oily component of the droplets is preferably a triglyceride containing oleic acid and / or linoleic acid. The nanoemulsions are used for delivering into cells the lipophilic substance contained by the oily droplets. This delivery can be used to biotransform the lipophilic substance, promote cell differentiation, growth or biosynthesis of a desired substance, or to test for toxicity of the lipophilic substance.

New thyroid receptor ligands are provided which have general formula (I) in which: n is an integer from 0 to 4; R1 is halogen, trifluoromethyl, or alkyl of 1 to 6 carbons or cycloalkyl of 3 to 7 carbons; R2 and R3 are the same or different and are hydrogen, halogen, alkyl of 1 to 4 carbons or cycloalkyl of 3 to 5 carbons, at least one of R2 and R3 being other than hydrogen; R4 is a carboxylic acid amide (CONR′R″) or an acylsulphonamide (CONHSO2R′) derivative, or a pharmaceutically acceptable salt thereof, and all stereoisomers thereof; or when n is equal to or greater than one, R4 may be a heteroaromatic moiety which may be substituted or unsubstituted, or an amine (NR′R″). R5 is hydrogen or an acyl (such as acetyl or benzoyl) or other group capable of bioconversion to generate the free phenol structure (wherein R5=H). In addition, a method is provided for preventing, inhibiting or treating a disease associated with metabolism dysfunction or which is dependent upon the expression of a T3 regulated gene, wherein a compound as described above is administered in a therapeutically effective amount. Examples of such diseases associated with metabolism dysfunction or are dependent upon the expression of a T3 regulated gene include obesity, hypercholesterolemia, atherosclerosis, cardiac arrhythmias, depression, osteoporosis, hypothyroidism, goiter, thyroid cancer as well as glaucoma, congestive heart failure and skin disorders.

The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and / or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and / or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

The present invention provides means for the production of desired end-products of in vitro and / or in vivo bioconversion of biomass-based feed stock substrates, including but not limited to such materials as starch and cellulose. In particularly preferred embodiments, the methods of the present invention do not require gelatinization and / or liquefaction of the substrate.

The invention relates to a method for cultivating tricholoma lobayense by utilizing fungus dregs in the edible fungus production field, which is characterized by utilizing the leftovers, namely fungusdregs, of the edible fungi instead of cottonseed hulls to cultivate the tricholoma lobayense, utilizing the method of biological fermentation to treat the fungus dregs and adding bran and other nutrients to form culture media, and effectively realizing cultivation of the tricholoma lobayense through the process flows of bagging, sterilizing, inoculating, hypha fermenting, cultivating, bag removing, soil covering, fruiting and harvesting, etc., wherein, the leftovers of the edible fungi are used for cultivating oyster mushrooms and needle mushrooms, etc. The method not only realizes resource of the organic wastes and reduces pollution of the organic wastes on the environment by developing and utilizing the leftovers of the edible fungi and conforms to the requirement on striving to developcircular economy encouraged by the state, but also greatly reduces the usage amount of the cottonseed hulls and other substances for cultivation, effectively lowers the production cost of the tricholoma lobayense, increases the cultivation benefits and ensures the biological conversion ratio to be more than 80%. The method utilizes the wastes and attains two objectives, thus being suitable for popularization and application in the rural areas.

The invention provides a formula of a culture medium for industrial production of sparasis crispa and a production process. The formula of the culture medium comprises the following components in percentage by dry weight: 65 to 75 percent of pine sawdust, 10 to 20 percent of husk, 5 to 10 percent of potato powder, 5 to 10 percent of flour, 0.1 to 0.5 percent of peptone, 0.1 percent of ammonium sulfate and 1.2 to 1.8 percent of brown sugar. The production process comprises the following steps of: preparing and bagging the culture medium; sterilizing and inoculating; culturing hypha; inducing a primodium; differentiating the primodium; opening the bag; managing balls; and harvesting. The invention provides a formula of a culture medium for industrial production of sparasis crispa, which has the advantages of rich nutrition, balanced proportion, good ventilation, and capacity of meeting the requirement of industrial production. The production process has the advantages of short hypha culturing time, uniform fruiting at bag openings, good fruiting consistency, convenience of management, contribution to industrial culture, white mushroom body, high yield, and high biological transformation rate of over 43 percent.

Acidic metal-bearing wastewaters are treated to produce a finished water of sufficient purity to meet discharge standards while recovering metals removed in forms which are commercially valuable. The metals are selectively precipitated, either in a batch or in a continuous system, for removal of individual metal products in a specific sequence of steps from the wastewater. In each step, the pH is adjusted to the specific pH range and sulfide ion is introduced to precipitate the metals, excepting the removal of ferric iron and aluminum which is achieved using hydroxide precipitation. Bioconversion process using unique equipment converts sulfate in the wastewater to the hydrogen sulfide gas required for the precipitation process. This bioconversion process reduces the sulfate in the wastewater so that the water can be directly discharged or used for agricultural applications.

This invention is a process for managing the gas flow through a plurality of bioconversion modules that provide a gas liquid interface. The conversion modules provide the gas liquid interface across an activated surface that converts at least some of the gas components into desired liquid products. Arrangement of the modules and control of gas flow in accordance with this invention enhances the utilization of the gas and the production of desired liquid products by adjusting the flow area to compensate for changes in the volume of the feed gas. Improved control of the gas velocity through the bioconversion modules eliminates problems of liquid condensation and flow maldistribution. The process may sequence the modules to mitigate time variation in microorganism activity and incorporate additional periodic process steps.

The invention discloses a corayceps militaris industrial cultivation method. The corayceps militaris is cultivated in an industrial way by strain preparation, blending, bottling, sterilizing, inoculation, culture, germination and collection; fimilar artificial substitute materials are used as the culture materials, which reduce manufacture cost. In addition, the corayceps militaris obtained by the invention has the characteristics of good quality stability and high biological percent conversion, etc.

The invention provides a breeding method of selenium enriched black soldier fly larvae. Inorganic selenium or organic selenium is added into feed of the black soldier fly larvae and the concentration of selenium content in the feed of the black soldier fly larvae is controlled at 10 to 80 mg every kg. The selenium is added into the breeding feed of the black soldier fly larvae to obtain the high selenium content of black soldier fly larva products and accordingly the functional value of the black soldier fly larvae is significantly improved. The selenium is combined with protein and polysaccharide organically in the black soldier fly larvae after bioconversion and accordingly the toxic and side effect and the gastrointestinal stimulation of the chemical selenium are eliminated and the selenium can be absorbed and utilized efficiently and securely.

The invention relates to an edible fungus compost, a production method thereof and an edible fungus culture process. The edible fungus compost disclosed by the invention consists of 40 to 90 parts of residual waste (spent mushroom substrate) left over after the harvest of edible fungi, 5 to 40 parts of cottonseed hulls, 3 to 20 parts of bran, 2 to 8 parts of corn meal, 1 to 2 parts of gypsum powder, 0 to 5 parts of quicklime, 0 to 2 parts of superphosphate and 0.05 to 0.8 parts of fungus-strengthening substance, and the pH value is 6 to 9. The invention adopts the residual waste (spent mushroom substrate) left over after the harvest of edible fungi as main material ingredient to produce the edible fungus compost. By implementing the invention, the residual waste of edible fungi cannot pollute the environment any more, and is made profitable, and the resource can be recycled; an edible fungus material resource is added, the economic burden caused by the shortage and higher prices of edible fungus materials is decreased for edible fungus farmers, the production cost of the method is reduced by more than 50 percent in comparison with the production cost of the conventional method, the yield is increased by 10 to 15 percent, the bioconversion rate reaches 80 to 120 percent, and economic income and social benefit are increased. The method is applicable to a variety of edible fungi.

The bioconversion of gas feedstreams to liquid products by direct contact with a layer of microorganism obtains enhanced productivity through the regular cycling of liquid across a substrate that supports a biolayer of microorganisms while separating the gas and liquid phases. Such processes produce liquid products such as ethanol, butanol and other chemicals from syngas components by contacting CO or a mixture of CO2 and H2 with a highly porous side of an asymmetric membrane under anaerobic conditions and transferring these components into contact with microorganisms contained within bio-pores of the membrane. A periodic laving of liquid from the liquid contact side to and away from the microorganisms can increase nutrient flow to the microorganisms while enhancing the recovery of liquid products. The process effects laving by temporarily raising the liquid phase pressure relative to the gas phase pressure to periodically surge liquid toward the microorganisms and then restoring the original pressure differential to urge liquid away from the microorgaisms.

The invention discloses a quick and intensive straw management method based on ecological conversion via housefly metabolism. The method comprises the following steps of: housefly species collection and clean breeding and acclimatization, induction and stabilization of straw conversion adaptation housefly strains, breeding of cellulose high expression housefly strains, pretreatment of straw housefly maggot bioconversion, stabilization of industrialized straw conversion housefly strains, and production of industrialized housefly maggot products. Compared with the prior art, the method has the advantages that biotechnologies are adopted for functional induction and filtration of seed maggot straw ecological conversion, passage and engineered cultivation, a machinery crushing method and a biofermentation technology are adopted, and a modernized engineering means is adopted, so that intensive straw management based on microbe fermentation and insect metabolic conversion is realized. Compared with other methods, the method provided by the invention has the advantages of high ecological degree, high bioavailability, short rotten period, high product quality, high extra-value output of resources, non-environmental pollution, and scientific proportion and high fertilizer efficiency.

An Intermodal container, also known as an ISO Ocean Freight Container is suitable for the dual purposes of bio-containment and also Intermodal shipping. Provision is made for the introduction of water, oxygen or other liquids or gasses to be treated into the container, for exit of liquids or gasses generated or converted in the container, and for the monitoring of biological processes occurring within the container, all in a liquid impervious container. Improved air ducts and flow paths are provided, while the effects of shrinkage and channeling are reduced, through a strategically placed and relative percentage of openings through the floor. Further improvements in air distribution are achieved by an elimination or substantial reduction in percentage of opening through the floor in areas immediately adjacent to the container side walls. The flooring incorporates a self-cleaning hole design which increases in diameter with increasing distance from the container contents. The increases in diameter may be achieved either through smooth and continuous increases, or through discontinuous increments, though the objective regardless of the approach is to ensure that the holes do not clog either when the container is filled with matter, or when vibrated and jolted during handling and transport.

The present invention relates to a flavoured milk essence. Said flavoured milk essence is made up by using butter as raw material and making it undergo two reaction processes of biological conversion and autothermic cracking. Said invention also provides the concrete operation steps of said two reaction processes.

The invention discloses an in situ biological repairing method of biomass strengthening petroleum -salt-mixed contaminated soil, which is to add biomass degradation agent into biomass, and then the biomass degradation agent is plowed and landfilled under plough horizon, water logging and salt washing are utilized to reduce soil salt content, and then petroleum hydrocarbon degradation bacteria agent is added to degrade petroleum hydrocarbon, The invention utilizes the biomass to interdict capillary salt uprise phenomenon possibly generated; carbohydrate substance generated by biomass degradation can be taken as a good quality carbon source of the petroleum hydrocarbon degradation bacteria agent, so as to accelerate the growth of the petroleum hydrocarbon degradation bacteria agent; lignin generated by the biological degradation can absorb petroleum hydrocarbon pollutant, to prevent being diffused in the water logging and salt washing, bio surfactant generated by microorganism in the petroleum hydrocarbon degradation bacteria agent can accelerate the digestion of the petroleum hydrocarbon pollutant and further be converted into carbon dioxide and water by the petroleum hydrocarbon degradation bacteria agent biology. The efficiency of the degradative petroleum hydrocarbon pollutant of the invention method is high, and the speed is quick.

The present invention pertains to methods for pretreatment of cellulosic biomass for bioconversion into ethanol and other biofuels and wood-based chemicals, recycling of newsprint and other paper products, microfibrillation of cellulose for use as an additive in the food and cosmetic industries, manufacturing improved hardboard, and producing and improved “super” pulp while reducing chemical usage and spent liquor generation. In particular, the instant invention employs supercritical, critical or near critical fluids with and without polar cosolvents [critical fluid, SuperFluids or SFS] for the pretreatment of cellulosic biomass.

Method of bioconversion of coal to methane, carbon dioxide, and other valuable gaseous and liquid products in a multi-step process that may include particle size reduction, separation of non-coal materials, addition of chemicals, and multi-stage anaerobic fermentation are disclosed.

The invention concerns a method for producing dicarboxylic acids (DCA) with long hydrocarbon chains, also called diacids, which consists in culturing a mutant strain of Yarrowia lipolytica obtained by mutagenesis directed and more particularly disrupted at least for the POX2, POX3, POX4 and POX5 genes encoding acyl-CoA oxydase, in a medium consisting essentially of an energetic substrate including at least one carbon source and one nitrogen source and in subjecting said strain to a bioconversion substrate selected among n-alkanes of at least 10 carbon atoms, fatty acids of at least 10 carbon atoms, their alkyl esters and natural oils.

The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.