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48results about How to "High protein activity" patented technology

Method for the treatment of fabry disease using pharmacological chaperones

The present invention provides a method treating a patient with Fabry disease by determining whether there is an improvement of a surrogate marker that is associated with Fabry disease following administration of a specific pharmacological chaperone of α-galactosidase A. The method includes administering an effective amount of 1-deoxygalactonojirimycn to the individual, wherein the 1-deoxygalactonojirimycin binds to alpha-galactosidase A in an amount effective to increase activity of the alpha-galactosidase A. The present invention also provides a method for monitoring and increasing a therapeutic response of a patient with Fabry disease following administration of a specific pharmacological chaperone of α-galactosidase A by evaluating the effect on the cytoplasmic staining pattern of a cell from the patient, wherein detection of a staining pattern in the cell that is similar to the staining pattern in a cell from a healthy individual indicates that the individual with Fabry disease is a responder.
Owner:AMICUS THERAPEUTICS INC

Process for producing l-amino acid

ActiveUS20120015409A1Highly productive in producingEfficient productionBacteria peptidesFermentationMicroorganismL-amino acid transport
The present invention provides a method of improving efficiency of a fermentative production of an L-amino acid. To be specific, the present invention provides a process for producing a L-amino acid, comprising culturing a microorganism wherein the activity of the protein described in any one of (1) to (3) below is higher than that of the parent strain in a medium to produce the L-amino acid and accumulate the L-amino acid in the medium, and then collecting the L-amino acid from the medium:(1) a protein comprising the amino acid sequence shown by any one of SEQ ID NOS:2, 4, 6 and 8(2) a protein consisting of the amino acid sequence resulting from deletion, substitution or addition of one or more amino acids in the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity(3) a protein consisting of the amino acid sequence having 80% or more homology to the amino acid sequence shown in any one of SEQ ID NOS:2, 4, 6 and 8, and having L-amino acid transport activity.
Owner:KYOWA HAKKO BIO CO LTD

Method for separating and purifying soybean agglutinin from soybean whey

The invention relates to a method for separating and purifying soybean agglutinin from soybean whey and belongs to the field of processing of agricultural products and comprehensive utilization of byproducts. The method disclosed by the invention comprises the following steps: (1) pretreating soybean whey; (2) primarily separating soybean whey protein; (3) re-agglomerating the soybean whey protein and poly-anions; and (4) recycling the soybean agglutinin. Raw materials are pretreated, primarily separated and then re-agglomerated to obtain a final protein polysaccharide compound; the final protein polysaccharide compound is ultra-filtered to remove sugar and then is secondarily centrifuged to obtain a protein solution containing purified soybean agglutinin; the protein solution is subjected to vacuum freeze-drying to obtain a high-purity sample of the soybean agglutinin. The method disclosed by the invention can be used for comprehensively utilizing the soybean whey and is low in requirements on equipment, simple to operate and free of environmental pollution; the soybean agglutinin is high in recycle rate, high in purity and high in protein activity and still retains natural physiological activity.
Owner:JIANGNAN UNIV

Using high-pressure homogenizing technology to process fresh plasma and fresh erythrocyie separated from animal blood

The invention discloses a blood plasma albumen and / or blood corpuscle albumen preparing method, which adopts a high pressure homogenizing machine to dispose the fresh animal blood plasma and / or blood corpuscle under the pressure of 60 to 120 MPa and mechanically sterilize the pathogen microbe and lead to the fresh blood corpuscle cell membrane breaking. The generated blood plasma albumen powder and / or blood corpuscle albumen powder have high security and greatly improve the digestion and absorption rate.
Owner:SHANGHAI GENON BIOLOGICAL PROD

Antibody conjugates of immune-modulatory compounds and uses thereof

Antibody conjugates of immune-modulatory compounds and pharmaceutical compositions for use in the treatment of disease, such as fibrotic diseases, autoimmune, or autoinflammatory diseases, are disclosed herein. The disclosed conjugates are useful, among other things, in treating fibrotic diseases, autoimmune diseases, or autoinflammatory diseases, such as by modulating TGFβR1, TGFβR2, TNKS, TNIK, or mTOR.
Owner:SILVERBACK THERAPEUTICS INC

Antibacterial peptide mixed solution and method for preserving freshness of foods by antibacterial peptide mixed solution

The invention belongs to the technical field of microbial resource utilization, in particular relates to a method for preparing an antibacterial peptide mixed solution generated by lactococcus lactis subsp lactis and lactobacillus sake, and relates to an application of the antibacterial peptide mixed solution in beancurd preservation. The method comprises the following steps of: preserving freshness of beancurd by adopting 10wt% antibacterial peptide mixed solution, and uniformly spraying the antibacterial peptide mixed solution on the surface of a beancurd block in a ratio of adding 2ml of antibacterial peptide mixed solution in every 10g of beancurd. According to the antibacterial peptide mixed solution, the sense score of the beancurd can be effectively prevented from being reduced, the growing speed of spoilage microorganisms can be inhibited, and the growth of total number of bacteria in the beancurd in the storing period can be suspended. Therefore, the antibacterial peptide mixed solution provided by the invention plays an active effect in beancurd preservation, and has better practical value.
Owner:ANHUI AGRICULTURAL UNIVERSITY

Chimeric antigen receptor T cell and application thereof

The invention provides a chimeric antigen receptor T cell and application thereof. The chimeric antigen receptor T cell expresses chimeric antigen receptors targeting CD19 and PD-L1 at the same time.The chimeric antigen receptor T cell expresses a CD19 CAR structure and a PD-L1 CAR structure at the same time, and after PD-L1 CAR is combined with PD-L1 expressed by a tumor cell, an activation signal can be transmitted intracellularly. Therefore, the chimeric antigen receptor T cell not only can recognize the tumor cells expressing CD19 in a targeted manner, but also can relieve immunosuppression of PD-1 expressed by the tumor cells on the T cells, avoid an immune escape mechanism of the tumor cells and relieve the problem of drug resistance of CAR-T treatment; and a new idea is provided for CAR-T treatment and adoptive feedback immune cell treatment.
Owner:格源致善(上海)生物科技有限公司

Method of regulating cftr expression and processing

InactiveUS20160108406A1Expression of target gene can be increasedHigh expressionOrganic active ingredientsMedical devicesCell biologyΔf508 cftr
The present invention relates to methods of reducing ΔF508-CFTR ubiquitination or degradation, or increasing ΔF508-CFTR processing or function in a CF cell comprising contacting the cell with a therapeutic agent that inhibits NEDD8, FBXO2, and / or SYVN1 expression in the cell.
Owner:UNIV OF IOWA RES FOUND

Method for separating and purifying sea urchin multifunctional actin

The invention discloses a method for separating and purifying sea urchin multifunctional actin. The method is characterized by comprising the following steps: (1) taking coelomic fluid of sea urchin, and centrifuging to take the supernatant so as to obtain the coelomic fluid supernatant; (2) taking the coelomic fluid supernatant as a sample to carry out native non-reductive dialysis electrophoresis; (3) carrying out native non-reductive polyacrylamide gel electrophoresis after the dialysis electrophoresis; (4) carrying out gel cutting on the multifunctional actin strip according to the specific color of the sea urchin multifunctional actin strip after the native non-reductive polyacrylamide gel electrophoresis, and recovering the protein in the gel through a gel crushing dissolution and release method so as to obtain the purified sea urchin multifunctional actin. The method has the advantages that (1) the purified sample is simple and rapid to prepare; (2) the reagent and the equipment are simple in requirement, easy to operate, less in step and short in time; (3) the purified sample has high purity and high protein activity.
Owner:LIAONING OCEAN & FISHERIES SCI RES INST

Method for preparing recombinant human blood coagulation factor VIII

The invention belongs to the technical field of biological engineering, and relates to a method for preparing a recombinant human blood coagulation factor VIII, in particular to a method for culturing cells for expressing a recombinant human blood coagulation factor VIII by using a wave type bioreactor and separating and purifying the recombinant human blood coagulation factor VIII from a cell culture liquid. The WAVE bioreactor is high in mixing efficiency, sufficient in gas-liquid exchange, small in foam amount and low in shearing force, damage of paddles of a stirring type stainless steel reactor and foams to cells is avoided, and thus the cell state, the cell survival rate and the protein activity of the WAVE bioreactor are all superior to those of the stirring type stainless steel reactor.
Owner:NANJING SHUNXIN PHARM CO LTD OF CHIATAI TIANQING PHARM GRP +1

Process for making bean curd by household soybean milk machine

The invention relates to a process for making bean curd by a household soybean milk machine. The process at least includes the following stages: an all-wall breaking stage: 1, maintaining the pulp materials in a first temperature range of 70 DEG to 90 DEG C for 1-10 min, and 2, crushing the materials into a slurry by using the crushing tool at a first speed of 16000-35000 RPM; a boiling stage: heating the above slurry by a heating element until a cooked state; bean curd manufacture phase: first adding a brine agent to the slurry, continuing solidifying, and finally pressing. Compared with the prior art, the soybean milk for making bean curd does not need filtering, so that the bean curd has high yield; and the bean curd has more comprehensive nutrients, and fine taste.
Owner:JOYOUNG CO LTD

Treatment of neurodegenerative diseases

InactiveUS20090169649A1Reduce and inhibit phosphorylationReduces and prevents accumulationBiocideNervous disorderMedicineNeuro-degenerative disease
The present invention relates to the use of selenate or a pharmaceutically acceptable salt thereof in methods and compositions for enhancing the activity of the protein phosphatase PP2A. Methods of reducing phosphorylation of tau protein, inhibiting activity of GSK3 and treating or preventing neurodegenerative diseases are also described.
Owner:VELACOR THERAPEUTICS PTY LTD

Method for preparing ACE (angiotensin converting enzyme) inhibitory peptide by using shellfish meat

The invention provides a method for preparing ACE (angiotensin converting enzyme) inhibitory peptide by using shellfish meat. The method has the advantages that the utilization rate and extraction rate of enzyme are improved, the activity of the ACE inhibitory peptide is high, and the good blood pressure reducing effect is realized. The method comprises the following steps of fermenting the shellfish meat by bacillus subtilis and aspergillus niger, and assisting by magnetic carbon microspheres, so as to improve the activity and purity of the ACE inhibitory peptide, and improve the fermenting rate; finally, purifying the ACE inhibitory peptide by a magnetic silicon dioxide adsorption column, so as to obtain the product. The prepared ACE inhibitory peptide has the advantages that the activity is high, the inhibition rate is high, and the good blood pressure reducing function is realized.
Owner:欧美生物技术孵化转移中心香港有限公司

Anti-I type diabetes fuse protein and preparation thereof

InactiveCN101353663AStable gene expression productRelieves pain and the threat of infectionPeptide/protein ingredientsMetabolism disorderDrugRecombinant virus
The invention relates to a fusion protein of anti-I type diabetes, and a preparation method thereof; the invention provides a fusion gene and a fusion protein which is expressed by the fusion gene; the fusion gene is nucleotide sequence which is shown by SEQ ID NO: 1. the invention also constructs an expression vector containing the fusion gene and a recombinant virus containing the expression vector; the fusion protein provided by the invention can be used for preparing the drugs for curing the I type diabetes.
Owner:ZHEJIANG UNIV

Methods for predicting protein activity based on identification of multidimensional signatures

The present invention provides a method for predicting antimicrobial activity of a candidate protein by determining the correlation between a multidimensional antimicrobial signature and a multidimensional antimicrobial signature model. The invention also is directed to a method for identifying a protein having antimicrobial activity by screening a library of candidate proteins to identify a multidimensional antimicrobial signature. Also provided by the invention is a method for improving the antimicrobial activity of a protein by altering the multidimensional antimicrobial signature of the protein to increase the similarity to a multidimensional antimicrobial signature model.
Owner:LOS ANGELES BIOMEDICAL RES INST AT HARBOR UCLA MEDICAL CENT

Methods of commercial production and extraction of protein from seed

InactiveUS7179961B2High activity of proteinIncrease recovery of proteinHydrolasesOther foreign material introduction processesCost savingsAgronomy
A method for extraction of heterologous protein from monocotyledonous plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.
Owner:PRODI GENE INC

Grape branch fermented feed preparation process and equipment based on biological fermentation technology

The invention provides a grape branch fermented feed preparation process and equipment based on a biological fermentation technology. The preparation process mainly comprises the following steps of S1, washing and soaking collected grape branches, and introducing ozone water to eliminate pesticide residues; S2, crushing the pretreated grape branches, performing high-pressure sterilization, and adding a mixed bacterium suspension for modified fermentation; S3, premixing the modified grape branch powder with cottonseed meal, fish meal, meat and bone meal, distillers' grains, rice bran, bagasse,wheat bran, corn flour and calcium hydrophosphate, and then adding fermentation liquor; and S4, stacking the fermented materials into long piles with the same length and the same height at the bottomof a fermentation chamber, performing covering fermentation, and conveying the fermented grape branch fermented feed into a granulator through a conveying device for granulation. In conclusion, the method has advantages of advanced process, perfect scheme, novel equipment and the like.
Owner:西安若翰生物饲料有限公司

Recombinant chromatin modified albumin 1A, as well as coding gene and application thereof

The invention discloses a recombinant chromatin modified albumin 1A, as well as a coding gene of and an application of the recombinant chromatin modified albumin 1A, wherein the recombinant chromatin modified albumin 1A comprises chromatin modified albumin 1A, the C end of the chromatin modified albumin 1A is connected with His-Tag, the N end of the chromatin modified albumin 1A is connected with a PTD (protein transduction domain) sequence, the amino acid sequence of the PTD sequence is YGRKKRRQRRR, and the amino acid sequence of the recombinant chromatin modified albumin 1A is shown as SEQ ID NO.2. According to the recombinant chromatin modified albumin 1A, an HIS-Tag tag is guided into wild type Chmp1A, so that the structure of the Chmp1A can not be changed, and can be easily purified through a Ni-NTA (nitrilotriacetic acid) Argarose albumen purifying system. The PTD sequence is guided into the wide type Chmp1A, so that the Chmp1A can effectively enter into tumor cell under the guide of the PTD, and the antitumor activity of the Chmp1A can be played.
Owner:ZHEJIANG ACAD OF MEDICAL SCI

PLA2R, C1q and THSD7A fusion protein and construction method and application thereof

The invention discloses a PLA2, C1q and THSD7A fusion protein and a construction method and application thereof. The construction method comprises the following steps that S1, PLA2R, THSD7 and C1q nucleotide sequences are sequentially connected to obtain a target gene, C1q is repeated three times, Linker sequences are added between PLA2R and THSD7A, Linker sequences are added between THSD7 and C1q, and Linker sequences are added between adjacent C1q; S2, an eukaryotic KOZAK sequence is added before the target gene, a restriction endonuclease site Bam I is added at the upstream, a Not I enzymedigestion site is added at the downstream, a 6* His tag sequence is added at the C end, and a complete sequence gene is designed according to mammalian cell preference; S3, the complete sequence geneobtained in the step S2 and plasmids are subjected to double enzyme digestion to obtain an enzyme digestion product; S4, the enzyme digestion product obtained in the step S3 is connected through T4 ligase to obtain recombinant plasmids; S5, the recombinant plasmids are expressed in mammalian cells to obtain the PLA2R, C1q and THSD7A fusion protein. The fusion protein constructed by the method canimprove the sensitivity and specificity of a detection kit and reduce missed detection and false detection.
Owner:苏州携创生物技术有限公司

Membrane scaffold protein, phospholipid nanodisk and nanoparticle as well as preparation methods thereof

In the embodiment, the invention provides a membrane scaffold protein, a phospholipid nanodisk and a nanoparticle as well as preparation methods thereof. The membrane scaffold protein has an amino acid sequence shown in SEQ ID NO. 1; the phospholipid nanodisk is a discoid phospholipid bilayer, and is prepared from the following raw materials: the membrane scaffold protein, a lipid solution and a buffer solution, wherein the lipid solution is prepared by dissolving lipid with a 50-100 mM sodium cholate solution until the final concentration of 45-55 mM is reached; and the nanoparticle is prepared from the following raw materials: the phospholipid nanodisk, and membrane protein. According to the embodiment of the invention, by preparing the nanoparticle from the phospholipid nanodisk and themembrane protein, relatively high protein activity can be kept for the membrane protein, thereby having related biological functions of the membrane protein retained; and thus, the membrane protein purified by the method has high purity and high activity.
Owner:CUSABIO TECH LLC

59R mutant vector for expressing rFC protein as well as preparation method and application of 59R mutant vector

The invention relates to the technical field of transgenosis, in particular to a vector for expressing rFC protein as well as a preparation method and application of the vector. According to the vector, an sNT sequence is mutated, so that sNT is insensitive to pH, and the problem of insolubility caused by fibrosis of foreign protein in a silk gland cavity in a pSG expression system is effectivelysolved. The condition that an sNT monomer forms a dimer at a low pH value, can be effectively avoided. The soluble expression capability and the protein activity of rFC and other foreign proteins mediated by the vector in a pSG bioreactor are improved. The method is a simple and efficient rFC protein production method, and the protection on endangered wild animals is effectively improved.
Owner:BEIBU GULF UNIV

Preparation method of milk product with function of lowering blood pressure

The invention provides a preparation method of a milk product with the function of lowering blood pressure. The milk product with the function of lowering blood pressure is prepared by adding a compound of ACE inhibitory peptides prepared by adopting shellfish meat and green tea powder into a milk product; moreover, enzyme utilization rate and extraction rate are improved by the preparation methodso that the ACE inhibitory peptides are high in activity. According to the preparation method, the shellfish meat is fermented by utilizing bacillus subtilis and aspergillus niger; magnetic carbon microspheres are utilized so as to assist to improve activity and purity of the ACE inhibitory peptides, and thus, fermentation rate is improved; and finally, the ACE inhibitory peptides are purified byusing a magnetic silica adsorption column so as to obtain a final product. The ACE inhibitory peptides prepared according to the preparation method are high in activity and inhibition rate, and havea very good effect of lowering blood pressure; and thus, the milk product prepared by adding the ACE inhibitory peptides has a very good effect of lowering blood pressure.
Owner:周文辽
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