84results about How to "Relief of immunosuppression" patented technology

Belonging to the field of tumor treatment and molecular immunology, the invention relates to an anti-CTLA4-anti-PD-1 bifunctional antibody, a pharmaceutical composition and use thereof. Specifically, the anti-CTLA4-anti-PD-1 bifunctional antibody comprises: a PD-1 (programmed death-1) targeted first protein functional region, and a CTLA4 (cytotoxic T lymphocyte sociated antigen 4) targeted second protein functional region. The bifunctional antibody provided by the invention can well bind to CTLA4 and PD-1 specifically, specifically remove the immunosuppression of CTLA4 and PD-1 on the body, and activate T lymphocytes, thus having good application prospect.

The invention belongs to the field of oncotherapy and molecular immunology, and relates to a PDL-1 antibody, a pharmaceutical composition thereof and application of the PDL-1 antibody, in particular to a monoclonal antibody of PDL-1 or an antigen binding fragment thereof. A variable region of the heavy chain of the monoclonal antibody comprises CDR of which the amino acid sequence is SEQ ID NO: 15-17; and a variable region of the light chain of the monoclonal antibody comprises CDR of which the amino acid sequence is SEQ ID NO: 18-20. The monoclonal antibody can be well combined to PDL-1 specifically, inhibition of PDL-1 to body immunity is relieved specifically, and T lymphocyte is activated.

The present invention belongs to the field of tumor treatment and molecular immunology, and relates to an anti-PD1 monoclonal antibody, a pharmaceutical composition and uses thereof. In particularly, the present invention relates to a monoclonal antibody or an antigen-binding fragment thereof, wherein the heavy chain variable region of the monoclonal antibody comprises CDR having an amino acid sequence represented by SEQ ID NO:9-11, and / or the light chain variable region of the monoclonal antibody comprises CDR having an amino acid sequence represented by SEQ ID NO:12-14. According to the present invention, the monoclonal antibody can well and specifically bind to PD1, can specifically release the immunosuppression of PD1 on body, and can activate T lymphocytes.

The invention belongs to the field of oncotherapy and molecular immunology, and relates to an anti-PD-L1 antibody as well as a pharmaceutical composition and an application of the anti-PD-L1 antibody. Particularly, the invention relates to a monoclonal antibody or an antigen binding fragment of the monoclonal antibody, wherein a heavy chain variable region of the monoclonal antibody comprises a CDR, the amino acid sequence of the CDR is SEQ ID NO:1-3, and / or a light chain variable region of the monoclonal antibody comprises a CDR, and the amino acid sequence of the CDR is SEQ ID NO:4-6. The monoclonal antibody is specifically bound with PD-L1 well, specifically relieves immunosuppression of PD-1 on the organism, and activates the T iymphocyte.

The invention belongs to the fields of oncotherapy and molecular immunology, and relates to an anti-PD-1 (programmed cell death 1) monoclonal antibody as well as pharmaceutical composition and an application thereof, in particular to a monoclonal antibody or an antigen binding fragment thereof. The heavy chain variable region of the monoclonal antibody contains CDR with amino acid sequences represented as SEQ ID NO: 13-15; and / or the light chain variable region of the monoclonal antibody contains CDR with amino acid sequences represented as SEQ ID NO: 16-18. The monoclonal antibody can be specifically bound with PD-1 very well, specifically remove immunosuppression of PD-1 on an organism and activate T lymphocytes.

The invention discloses a traditional Chinese medicine additive for preventing and treating porcine viral enteritis, which comprises the following raw materials: Astragalus membranaceus, Radix Codonopsitis, radix sileris, Poria cocos, Atractylodes macrocephala, aucklandia roots, Magnolia officinalis, evodia, dried ginger, Fructus Psoraleae, raw hawkthron, goose grasses, honeysuckle, isatis roots, swamp mahogany, dyers woad leaves, radix scutellariae, coptis roots, pawpaw, garlicin, borneol and liquorice. After being eaten by pigs, the traditional Chinese medicine additive can have the function of health protection, improve the immunologic function and the disease resistance of the pigs, and prevent and treat porcine viral enteritis, wherein the disease rate is 8 percent, and the mortality rate is 1 percent; and the effective rate for treating porcine viral enteritis is 98 percent, and the cure rate is 97 percent. The raw materials have wide source and low cost, and are safe and nontoxic; no drug resistance is generated; and pork has no drug residue, so that the traditional Chinese medicine additive for preventing and treating porcine viral enteritis is an ideal product for replacing the prior traditional Chinese medicine additive.

The invention discloses a Chinese herbal medicine premixed agent for curing swine high fever, which takes raw materials of traditional Chinese medicine such as astragalus root, codonopsis pilosula, honeysuckle flower, weeping forsythia, radix isatidis, folium isatidis, echinacea purpurea root, cicada shell, secretio bufonis, borneo camphor, vitamin C, enzymatic probiotics (licorice root, and medicated leaven), and garlicin, and the medicine is made into fine powder (200 orders) and is evenly mixed, 0.5 kilogram of the premixed agent is added to 100 kilograms of fodder ( basic daily grains) when in use. After the fodder is eaten by a pig, digestion and absorption of a pig can be advanced, appetite can be simulated, and growing speed can be increased, and daily weight gain reaches 1.81%, and the consumption of the fodder is reduced by 14%. The invention can play a role of health care, improve immune function and disease-resistant capacity of a pig, prevent and cure swine high fever reduce morbidity by 95%, and reduce death rate by 98%. And the invention has the advantages of extensive raw material source, low cost, and safety and innocuity, and does not generate drug resistance, which is an ideal Chinese herbal medicine premix substitution, and meat does not contain residual medicine.

A monoclonal antibody for PDL-1 (programmed cell death-ligand 1) or its antigen binding fragment is provided. The monoclonal antibody can specifically bind with PDL-1, inhibition of PDL-1 upon body immunity is resolved, and T-lymphocytes are activated. A pharmaceutical composition containing the monoclonal antibody and application of the monoclonal antibody are also provided.

The invention discloses eco-nutritional compound feed for laying hens at an egg-laying peak and a preparation method of the eco-nutritional compound feed. The eco-nutritional compound feed for the laying hens at the egg-laying peak comprises components in percentage by weight as follows: corn, bean pulp, rice bran, calcium hydrophosphate, rock flour, baking soda, sodium chloride, soybean oil, compound trace elements for the laying hens, compound vitamins for the laying hens, 50% choline chloride, a bacillus coagulans preparation, a fructo-oligosaccharide preparation, an DL-methionine, compound enzyme for the laying hens, phytase, montmorillonite and zeolite powder. The nutritional requirement of the laying hens can be met, the immunity and the anti-stress capacity of the laying hens can be effectively improved, the disease rate is reduced, the egg laying rate is increased, the lasting time of the peak is prolonged, the quality of eggs is improved, the environment of henhouses can be improved, and the eco-nutritional compound feed is a novel laying egg feed product integrating nutrition, the anti-disease property, promotion of a high yield and pollution-free property.

The invention relates to the field of molecular biology, in particular to a lentiviral vector of a CAR and PD1-CD28 chimeric receptor for simultaneous expression of targeted CD19 and CD20. Through theCAR and PD1-CD28 chimeric receptor which simultaneously efficiently expresses targeted CD19 and CD20, the lentiviral vector ensures that a carrier-infected T cell can not only realize targeted identification of a tumor cell which expresses CD19 and CD20 but also remove PD-L1 / PD1 mediated immunosuppression, so that a stronger tumor cell killing capability is achieved. Furthermore, selection and connection sequences of key elements in the carrier are optimized, and the expression efficiency of the CAR and PD1-CD28 chimeric receptor of the targeted CD19 and CD20.

The invention belongs to the field of tumor treatment and molecular immunology, and relates to an anti-VEGFA-anti-PD-1 bispecific antibody and an application thereof. Specifically, the anti-VEGFA-anti-PD-1 bispecific antibody comprises a PD-1-targeting first protein functional region and a VEGFA-targeting second protein functional region, according to an EU numbering system, a heavy chain constant region of immune globulin contained in the bispecific antibody mutates at two sites of 234 and 235, and after mutation, the affinity constant of the bispecific antibody is reduced compared to the affinity constant of Fc [gamma] RI, Fc [gamma] RIIa, Fc [gamma] RIIIa and / or C1q before mutation. The bispecific antibody disclosed by the invention can be specifically combined with VEGFA and PD-1, specifically relieve immunosuppression of VEGFA and PD-1 on an organism and inhibit angiogenesis caused by tumors at the same time, and has a good application prospect.

The invention discloses a livestock compound medicine with anti-virus and immune regulation functions. The livestock compound medicine is prepared from astragalus root, codonopsis pilosula, angelica, hedyotis diffusa, honeysuckle, radix bupleuri, liquorice and Isoprinosine. The livestock compound medicine has the functions of invigorating spleen-stomach and replenishing qi, replenishing blood, invigorating the circulation of blood, strengthening spleen and tonifying lung, clearing away heat and toxic materials, protecting liver, anti-inflammatory and reinforcing the organism immunity and has excellent treatment effect on various immune suppression diseases of livestock. The livestock compound medicine can improve the organism immunity of livestock, reinforce the cell immunity of livestock, promote the antibody generation, eliminate the organism immunity inhibition and effectively prevent and treat the livestock virosis. After use, the livestock compound medicine can promote the lymphocyte proliferation, increase the phagocytotic ability of phagocyte, reinforce the organism immunity of livestock, eliminate the organism immunity inhibition and greatly reduce the injury of immunosuppressive diseases to the livestock organism. The livestock compound medicine has no medicine residue, meets the safety and health requirements of livestock foods and is convenient to use; the livestock compound medicine can be taken together with drinking water or can be mixed with feed without affecting the export of such animal products as livestock products.

The invention discloses a novel safe environmentally-friendly feed additive for the livestock and poultry industry. The novel safe environmentally-friendly feed additive is prepared from pheophytin, betaine, metaplexis japonica extract, oxidized matrine, yucca saponin, rose-boot extract, flos caryophylli powder, clinoptilolite powder and illite powder according to a scientific ratio. The novel safe environmentally-friendly feed additive has activation, adjustment and quick-acting functions, improves animal immunity, maintains the balance of florae in a livestock intestinal tract, improves muscle quality, a culture environment and an organic decomposition rate, reduces an ammonia concentration of air in an animal house and realizes deodorization. In breeding, the novel safe environmentally-friendly feed additive can fully avoid antibiotic medicines, reduce mycotoxins, prevent enterorrhea and diarrhea, resist stress and coccidium, improve a feed conversion ratio and an egg yield, and eliminate heavy metal and drug residues.

The invention provides a traditional Chinese medicine formula capable of improving animal immunity. The traditional Chinese medicine formula is prepared from, by weight, 25 parts of radix astragali, 25 parts of radix angelicae sinensis, 12.5 parts of herba epimedii, 25 parts of radix isatidis, and 12.5 parts of licorice. The invention also provides a preparation method of the traditional Chinese medicine formula. The preparation method comprises following steps: the ingredients are weighed at the above weight ratio, and are smashed using a pulverizer respectively; obtained powder products are sieved using a sieve of 150 meshes, and are mixed using a V-type mixer for 15min; and then an obtained product is subjected to subpackage, and is sealed. The traditional Chinese medicine formula is capable of repairing immune system and improving body immunity, releasing immunosuppression, improving immune effect, reducing medicine dosage, and extending drug application range, and is suitable to be applied to chicken, pigs, horses, cattle, sheep, ducks, geese, and rabbits.

The invention relates to the technical field of livestock breeding, and in particular, discloses a polypeptide composition, a composition for improving the livestock and poultry immune function, and a preparation method of the composition for improving the livestock and poultry immune function. The polypeptide composition is prepared from bursin peptide, thymosin and a transfer factor according to the mass ratio of (0.5-5):(0.5-5):(0.5-5). The composition for improving the livestock and poultry immune function is prepared from the following raw materials in parts by weight: 20-30 parts of B vitamins, 20-30 parts of vitamin C, 10-20 parts of the polypeptide composition, 10-15 parts of taurine, 1-2 parts of an anti-caking agent, and 40-50 parts of porous starch. The polypeptide composition and the composition can both be used as feed additives, or as pharmaceutical preparations to be added in drinking water, or as pharmaceutical preparations used for injection, have good prevention and control effects on swine fever, foot-and-mouth disease, circovirus, piglet diarrhea, Newcastle disease, mild type flu, duck liver virus disease and the like, reduce the use of antibiotics, reduce the occurrence of epidemic diseases, reduce mortality rate, and improve the immunity.

The invention relates to a medical composition and a mixture for treating chicken infectious bursal disease and a preparation method thereof. The medical composition (100 grams) comprises: 1 to 10 grams of astragalus polysaccharides, 3 to 12 grams of taurine, 4 to 15 grams of aminopyrine, 2 to 10 grams of vitamin C, 0.04 to 0.2 gram of interferon inducer, 5 to 20 grams of alcohol, 0.1 to 0.4 gram of sodium benzoate, and the balance of water for injection. The product is characterized by reasonable formula composition, stable physical and chemical properties, accurate dose, quick effect, long-lasting medicament effect, high bioavailability, safe use and exact treatment effect.

The invention discloses a novel plant active feed additive. The novel plant active feed additive is prepared from composite extract of triticale extract, dandelion, clinoptilolite, a modified microcrystalline silicate, plumepoppy, kelp, rosemary, clove, wolfberry, fructus psoraleae and garlicin according to a scientific ratio. The novel plant active feed additive has activation, adjustment and quick-acting functions, is rich in eighteen amino acids and a plurality of minerals, vitamins, flavonoids and saponins, fully avoids antibiotic drugs in breeding, eliminates heavy metal and drug residues, reduces a concentration of ammonia in an animal house, improves a culture environment and animal immunity, maintains the balance of florae in a livestock intestinal tract, improves muscle quality, prevents enterorrhea and diarrhea and improves a feed conversion ratio and an egg yield.

The invention provides a traditional Chinese medicine fermented feed for preventing African swine fever and a preparation method of the traditional Chinese medicine fermented feed. The traditional Chinese medicine fermentation feed is prepared from a fermentation substrate and mixed bacterial liquid, and a weight ratio of the fermentation substrate to the mixed bacterial liquid is (60-70) to (30-40). The fermentation substrate is prepared from the following components in parts by weight: 55-65 parts of corn flour, 20-25 parts of soybean meal, 4 parts of 4% piglet premix, 1-3 parts of rhizoma coptidis, 3-5 parts of radix scutellariae, 3-5 parts of cortex phellodendri and 2.5-3.5 parts of fructus gardeniae. The mixed bacterial liquid comprises lactic acid bacteria, yeast and bacillus subtilis. The traditional Chinese medicine fermented feed can adjust the balance of gastrointestinal flora, improve the immunity of the organism and effectively prevent the African swine fever.

The invention discloses a decavitamin and fermented Chinese traditional medicinal herb preparation special for postpartum breeding sows. Every 1000 parts of Decavitamin and fermented Chinese traditional medicinal herb preparation special for the postpartum breeding sows comprises, by weight, 98.7-125.5 parts of decavitamin, 10-15 parts of compound amino acid, 95-120 parts of polysaccharide and 752.8-676.5 parts of fermented composite fine powder. The decavitamin and fermented Chinese traditional medicinal herb preparation special for the postpartum breeding sows can rapidly supplement the needed nutrients for the postpartum breeding sows, enhance the immunity of the sows and improve stress resistance and the disease resistance. The invention also aims to provide a preparing method and an application of the decavitamin and fermented Chinese traditional medicinal herb preparation.

The invention provides a traditional Chinese medicine feed additive capable of preventing sows from being subjected to abortion and having fetal death and a preparation method of the traditional Chinese medicine feed additive, and particularly relates to the technical field of feed additives. The traditional Chinese medicine feed additive consists of the following components of radix isatidis, honeysuckle flowers, radix scutellariae, radix astragali, bighead atractylodes rhizomes, epimedium, divaricate saposhnikovia roots, fineleaf schizonepeta herbs, yeast cell wall polysaccharide, glucose oxidase and anhydrous dextrose. The making technology of the feed additive sequentially comprises the steps of mixing traditional Chinese medicines, performing primary crushing, performing superfine crushing, adding a biological preparation and performing uniform mixing so as to obtain finished products. According to the traditional Chinese medicine feed additive and the preparation method thereof disclosed by the invention, a method combining the traditional Chinese medicines and the biological preparation is utilized, from the inspects of eliminating mildew, removing mildew, removing toxins, expelling toxins, nourishing the liver, protecting the kidney, strengthening vital qi to eliminate pathogenic factor and the like, firstly the danger of mycotoxin is removed, organs for removing toxinsand expelling toxins are repaired, the immune functions are improved, immunosuppression is removed, and the protection ratio of epidemic prevention is increased, so that the incidence of three greatpropagation obstacle diseases is reduced, the incidence rate of abortion, fetal death and weak fetuses is greatly reduced, and good effects are achieved.

The invention provides a method. The core of the method is an anti-human PD-L1 antibody, and the anti-human PD-L1 antibody and an anti-human CD3 antibody constitute the bispecific antibody. The bispecific antibody can bind T cells and express PD-L1 or inductively express PD-L1 tumor cells and kill bond tumor cells. The method can bypass limitation of TCR and MHC-I antigens and also solve immunosuppression caused by binding of PD-L1 with PD-1 on surfaces of T cells after inducted expression. Therefore, tumor cells can be more effectively killed and growth of tumor can be inhibited.

The invention relates to a traditional Chinese medicinal extract feed additive which is prepared from the following raw materials in parts by weight: 200-300 parts of a folium cortex eucommiae leaf extract, 80-120 parts of a soybean extract and 580-720 parts of corncob powder. All the components of the traditional Chinese medicinal extract feed additive work cooperatively, and the effects supplement each other and are scientifically matched; the content of a flavonoids compound serving as an effective component is high, and the flavonoids compound is natural, safe and free of side effect; and under the condition that drinking and intake of laying hens are not affected, the laying performance of the laying hens is effectively improved, so that the whole breeding benefit is enhanced. The invention further provides a preparation method for the traditional Chinese medicinal extract feed additive.

The present invention discloses a novel pig feed preparing process which relates to the technical field of livestock feed and the formula of the pig feed consists of polypeptides, corn protein powder, transfer factor lyophilized powder, rheum palmatum, baicalin and probiotics. The preparation process of the pig feed is as follows: the production temperature is controlled below 50 DEG C; firstly the polypeptides and the corn protein powder are premixed, and transfer factor lyophilized powder, rheum palmatum, baicalin are added, mixed and stirred thoroughly; and if the temperature of the stirrer rises after stirring, the probiotics are added and fully mixed after the temperature drops. The novel pig feed preparing process is mainly used for growing muscle and gaining weight of livestock and poultry, decomposing carcass androstenone and skatole, improving meat quality and increasing body resistance to diseases, improving feed conversion rate, reducing nitrogen emissions, improving pig house environment and increasing lean meat rate.

The invention provides an RNA chimera. SiRNA is specifically introduced into a T lymphocyte through the RNA chimera while other types of cells are not affected, so that the conditioned targeted therapy effect is guaranteed. The siRNA of PITPNM3 carried by a CD4 nucleic acid aptamer specifically silences expression of PITPNM3 in the T cell, the effect of CCL18 secreted by cancer related cells is blocked, the immunosuppression of breast cancer is relieved, tumor in situ grows slowly, and breast cancer metastasis is reduced.

The invention belongs to the field of tumor treatment and molecular immunology, and relates to an anti-PD-1 antibody and medical application thereof. Specifically, the invention relates to a monoclonal antibody. A heavy chain variable region of the monoclonal antibody comprises CDR with amino acid sequences of SEQ ID NOs:19-21; and / or a light chain variable region of the monoclonal antibody comprises CDR with amino acid sequences of SEQ ID NOs:22-24, wherein a heavy chain constant region of the antibody mutates at any two or three of a 234th site, a 235th site and a 237th site according to anEU numbering system, and the affinity constant of the mutated antibody and Fc[gamma]RIIIa and / or C1q is reduced compared with that before mutation. The monoclonal antibody can be well and specificallycombined with PD-1, specifically relieves the immunosuppression of the PD-1 on the body, and activates T lymphocytes.

The invention discloses a drug for removing toxins from the livestock body and improving immunity of the organism and a preparation method of the drug, and belongs to the technical field of livestockdrugs. The traditional Chinese medicine composition is prepared from radix astragali, herba taraxaci, flos chrysanthemi indici, folium eucommiae, rhizoma acori tatarinowii, gizzard membrane, bulbus lilii and poria cocos in the weight ratio being (10-20):(2-5):(4-10):(3-8):(2-8):(1-4):(2-6):(1-4):(2-5). The traditional Chinese medicine composition can regulate functions of an immune system comprehensively, relieve immunosuppression and improve disease resistance of livestock, has no toxic or side effects and is applicable to the livestock for a long time.

The present invention discloses a traditional Chinese medicinal herb preparation, which is prepared with ginseng, Chinese caterpillar fungus, lucid ganoderma, velvet antler, tortoise shell, beef tendon, astragalus root, divaricate saposhnikovia root, kudzuvine root, Indian buead, costus root, semen plantaginis, fried rhizoma atractylodis macrocephalae, medcinal evodia fruit, rhizoma zingiberis, malaytea scurfpea fruit , fossilizid, oyster, hawthorn fruit, goosegrass herb, indigowoad root , eucalyptus robusta smith, common andrographis herb, baical skullcap root, golden thread, borneol and liquoric root. The traditional Chinese medicinal herb preparation can promote infantile digestive absorption, stimulate appetite, protect health, enhance the immunologic function and disease-resisting capability of infants and prevent and treat infantile virus enteritis, and the effective rate can reach 95 percent and 98.6 percent in preventing and curing infantile virus enteritis. The traditional Chinese medicinal herb preparation, which has the advantages of safety, non-toxicity and no generation of drug fastness, is an ideal traditional Chinese medicinal herb preparation for preventing and curing infantile virus enteritis.

The invention discloses a method for inhibiting dimethylbenzanthracene-induced rat mammary cancer with fucoidan, which relates to the technical field of mammary cancer inhibition. Sixty SPF (Specific Pathogen Free ) grade female SD (Sprague-Dawley) rats are randomly divided into a blank control group, a model control group, a low-dose fucoidan intervention group and a high-dose fucoidan intervention group according to body weights, fifteen rats in each group, the rats are killed after sixteen weeks, the incubation period and tumor inhibition rate of the mammary cancer of each group of rats are calculated, and thymus indexes, spleen indexes and the like are calculated. ELISA (enzyme-linked immunosorbent assay) is adopted to assay the levels of interleukins IL-6, IL-10 and IL-12, interferon-Gamma and transforming growth factor-Beta in serum, and a flow cytometer is utilized to assay the activity of NK (natural killer) cells in the peripheral blood of the rats and the cell percentage of the T-lymphocytes subset. The method has the following advantages that the method can regulate the immunity of rats, enhance antitumor immunity and relieve the immunosuppression of tumors, and the regulation of the activity of immune cells by fucoidan is one of the mechanisms of fucoidan in inhibiting the occurrence of mammary cancer.

The invention discloses a preparation method of an interfering peptide targeting SARS-CoV-2N protein. The method comprises the following steps: designing an interfering peptide fragment targeting amino acid located in a dimerization structural domain of the SARS-CoV-2N protein; fusing the interfering peptide fragment with HIV-TAT (Human Immunodeficiency Virus-TAT); modifying the interfering peptide fragment fused with HIV-TAT into a retroisomer to obtain the amino acid sequence of the final interfering peptide NIP-V; and synthesizing the interfering peptide NIP-V by using D-type amino acids as raw materials. The interfering peptide drug NIP-V can interact with the dimerization structural domain of the SARS-CoV-2N protein to inhibit oligomerization of the N protein, so that inhibition of the N protein on innate immunity is relieved, and the purpose of inhibiting replication of the SARS-CoV-2 virus in cells and animal bodies is achieved.