The invention provides a Chinese cabbage isolated microspore inducing medium. According to the formula, the medium contains KNO3, MgSO4-7H2O, KH2PO4, Ca(NO3)2-4H2O, Na2-EDTA, FeSO4-7H2O, MnSO4-4H2O, AgNO3, H3BO3, ZnSO4-7H2O, KI, Na2MoO4-2H2O, CuSO4-5H2O, CoCl2-6H2O, L-glutamine, vitaminB1, vitaminB6, nicotinic acid, folic acid, biotin, inositol, glycine, proline, serine, L-glutathione, methylnitrosourea, sucrose, phytagel, 6-BA, complex peptide nucleic acid, protein hydrolysate, colchicines, activated carbon and plant vulcanized kinetin PSK-alpha. The medium can greatly improve the inducing efficiency of Chinese cabbage isolated microspore embryonic calluses and has important application value.