174results about How to "Improve induction ability" patented technology

The invention relates to a preparation method of three-layer lead tissue regenerating velum with gradient, belonging to macromolecule biomedical material field. The velum is a three-layer composite velum made from natural biological material, degradable polymer and nanometer hydroxyapatite as raw materials. The method comprises steps of dissolving mixture of the natural biological material, degradable polymer and nanometer hydroxyapatite with different proportions in a special solvent; preparing the natural biological material / degradable polymer / nanometer hydroxyapatite three-layer gradient composite velum through static spinning layer by layer; and using pure natural biological material to modify surface of the velum by means of static self-assembly layer by layer. The velum has excellentbiological compatibility and mechanical property; one side of the velum is crude and loose, so the side can face to a defect area and is helpful for bones to absorb and grow and for blood coagulationblocks to stabilize; the other side is smooth and compact, so the side can face to surrounding tissue to prevent connective tissues from entering the defect area.

The present invention belongs to the field of tumor treatment and molecular immunology, and relates to an anti-PD1 monoclonal antibody, a pharmaceutical composition and uses thereof. In particularly, the present invention relates to a monoclonal antibody or an antigen-binding fragment thereof, wherein the heavy chain variable region of the monoclonal antibody comprises CDR having an amino acid sequence represented by SEQ ID NO:9-11, and / or the light chain variable region of the monoclonal antibody comprises CDR having an amino acid sequence represented by SEQ ID NO:12-14. According to the present invention, the monoclonal antibody can well and specifically bind to PD1, can specifically release the immunosuppression of PD1 on body, and can activate T lymphocytes.

An artificial promoter system that can be fused upstream of any desired gene enabling reversible induction or repression of the expression of the gene at will in any suitable host cell or organisms by light is described. The design of the system is such that a molecule of the plant photoreceptor phytochrome is targeted to the specific DNA binding site in the promoter by a protein domain that is fused to the phytochrome and that specifically recognizes this binding site. This bound phytochrome, upon activation by light, recruits a second fusion protein consisting of a protein that binds to phytochrome only upon light activation and a transcriptional activation domain that activates expression of the gene downstream of the promoter.

The invention discloses a genetic engineering application of nitrate transport protein genome OsNRT2.3 of paddy rice, pertaining to the genetic engineering field. The nucleotide sequence of the nitrate transport protein genome OsNRT2.3 of paddy rice is SEQ ID NO.1, and OsNRT2.3a protein amino acid sequence expressed thereby is SEQ ID NO.2 and OsNRT2.3b protein amino acid sequence expressed thereby is SEQ ID NO.3. The gene of the invention is a first report on paddy rice. By participation in the transport of nitrate of paddy rice and especially under the condition short of nitrogen and poor pHenvironment, the analysis of mRNA expression shows that OsNRT2.3a is induced by low nitrate nitrogen so that the expression only occurs in lateral roots; and OsNRT2.3b has expressions in root system and parts over the ground and is responsible for the transport of nitrate. The OsNRT2.3a and OsNRT2.3b proteins are expressed in a frogspawn heterogenous system and determined as high compatible nitrate transport protein. A pH regulation and control site is found to exist in both proteins which are regulated and controlled by pH. Under low nitrogen condition, OsNRT2.3 transgenic plant shows higherefficiency in transport of nitrate, thus improving the utilization efficiency of nitrogen and the ultimate output.

The invention discloses a stent for osteochondral defect repair. The stent consists of a cartilage layer with pores and a subchondral bone layer with pores, wherein the cartilage layer and the subchondral bone layer are tightly combined into a whole; the material of the cartilage layer is a mixture of type I collagen and chondroitin sulfate; and the material of the subchondral bone layer is porous titanium or porous titanium oxide or a porous titanium alloy. A preparation method of the stent comprises the following steps of: (1) processing the porous titanium or porous titanium oxide or porous titanium alloy to a shape and a size required by the subchondral bone layer; (2) preparing cartilage layer slurry; and (3) molding and treating a stent, putting the subchondral bone layer prepared in the step (1) into a mold, injecting the cartilage layer slurry prepared in the step (2) into the mold, putting the mold into a freeze dryer, freezing for at least 5 hours, molding, performing thermal crosslinking and chemical crosslinking on the molded stent, and putting the chemically-crosslinked stent into sterile water for rinsing to obtain the stent for osteochondral defect repair.

The MN / CA IX protein is identified herein as a hypoxia marker. The MN / CA9 gene promoter is characterized, and the location of the HIF-1 binding site within the MN / CA9 promoter is identified. Further, the hypoxia inducibility of the MN / CA9 gene and the uses of such inducibility are disclosed. In one aspect, the invention provides diagnostic / prognostic tools for determining the presence of hypoxia in a tissue in a vertebrate, preferably a human, and for measuring the relative degree of hypoxia in said vertebrate. In another aspect, the invention provides methods using tumor biopsies to predict the radioresistance of a preneoplastic / neoplastic tissue in a vertebrate subject, preferably a human patient, for diseases in which MN / CA IX levels can be used to indicate radiobiologically relevant tumor hypoxia. Such predictive methods can be used as an aid in patient therapy selection.

The present invention relates to a bio-active 3-D porous tissue engineering material and its preparation method. It uses the polylactate or polyethylene-propylene lectide, chitosan and its derivative, bovine serum albumin, growth factor and heparin as raw material, and adopts the supercritical CO2 fluid technology to prepare 3-D porous supporting material which can continuously release active growth factor. It has good mechanical property and biological compatibility.

The invention relates to a hemostatic gel suppository, which is characterized in that the hemostatic gel suppository is compounded by sodium carboxymethyl cellulose and other gels. The suppository has shape of hollow thin shell. The hemostatic gel suppository has the advantages that the hemostatic gel suppository has stronger induction function for the aggregation of platelet, can accelerate the deformation, adhesion, aggregation and release of the platelet so as to accelerate human physiological hemostasis, can activate blood clotting factors XII at a bleeding part to accelerate blood clotting, quickly absorbs water in blood and tissue fluid to form a gel closed blood capillary end face so as to function as mechanical blockage and stanch, can be firmly adhered to a wound surface to mechanically press the wound surface so as to stanch, does not adhere to the skin, has the function of inhibiting bacteria growth, and is accepted more easily by a patient.

The invention relates to a luminous induction system for rural road tunnels. According to the light environment characteristics of different sections of the tunnel, driving characteristics and visual characteristics of the driver, short tunnels are divided into short tunnels <200m and medium-short tunnels of 200-500m, and short tunnels of 200-500m are divided into: entrance section, middle section, In the exit section, multi-frequency and multi-size luminous induction facilities are set according to the characteristics of the road section; a reminder area is also set in the middle section; the high-frequency small-size induction information is set on the outside of the lane or at the maintenance road with the highest brightness, and the medium-frequency small-size induction information is set on The brightness of the side wall is lower than that of the curb induction information. The medium-scale induction information of the medium-frequency information is set on the side wall, and the low-frequency large-scale induction information is set on the side wall and extends to the top of the tunnel. Taking into account both safety and energy saving, while avoiding optical illusions and ensuring safety, it is especially suitable for short rural road tunnels (<500m) without electricity or lights.

The invention discloses a preparation method of antitumor adoptive immune cells. The method comprises the following steps of: sampling and separating a single peripheral blood karyocyte; and adding a cell factor for inducting and culturing to obtain a cytokine induced kill cell (CIK), wherein the cell factor comprises CD28. In a CIK induced system, the cell factor CD28 is induced, so that the inducing effect is enhanced, and the cell proliferation multiple is increased simultaneously.

The invention discloses a preparation method for a decellularized periosteal matrix gel material sourced from natural tissue. By conducting freeze drying on decellularized periosteal matrix sourced from mammal, bioactive molecules are kept to the maximum degree; the decellularized periosteal matrix gel material sourced from natural tissue is ground into powder, the grain size of the powder is controlled, the contact area of a solution is increased, in this way, digestion is promoted, and finally the standard and uniform decellularized periosteal matrix gel having a good osteogenic activity potential is obtained. In addition, EDC crosslinking growth factors have a good promoting effect when regenerated bone tissue is regenerated excellently and grows into with vascularization. The decellularized periosteal matrix gel material can be used for repairing bone defects, bone disunion and other kinds of bone regeneration obstacles caused by all kinds of clinical pathogeneses.

The invention discloses chitin organic fertilizer produced by using livestock excrement and shrimp and crab shells and a preparation method thereof. The chitin organic fertilizer produced by using the livestock excrement and the shrimp and crab shells comprises, by weight percentage, the following raw materials of 65 to 84 % of the livestock excrement, 5 to 15 % of waste of the shrimp and crab shells, 10 to 20 % of straw and 0.5 to 1 % of microbial agent. The chitin organic fertilizer has the advantages that a strong induction effect in the respect of restraining tobacco bacterial wilt is achieved, the tobacco is promoted to generate system resistance under the environmental stress of soil-borne disease, the soil biological activity can be promoted to improve at the same time, the occurrence of the conditions of tobacco vascular bundle deformation and xylem blocking can be reduced to a certain extent, therefore the efficient prevention and control over the tobacco bacterial wilt are achieved, and the problems that the organic fertilizer is slow in taking effect, the effect is not obvious after the fertilizer is applied, and the fertilizer performance is not stable are solved. In addition, the invention further discloses the preparation method of the chitin organic fertilizer.

The invention relates to a fluorescence analysis method for determining the concentration of Hg<2+> and belongs to the technical field of analysis detection. The fluorescence analysis method comprises the following steps: firstly, selecting two non-labeled single-chain DNA (Deoxyribonucleic Acid) molecules with different length and containing rich basic groups including T and G; preparing into solutions respectively; carrying out water-bath treatment for later use; mixing two types of solutions, a thioflavin solution and a Tris-HNO3 buffering solution to obtain a mixed solution; adding the Hg<2+> into the mixed solution, and shaking at a room temperature for 30min; determining a fluorescence intensity value under 450nm; calculating the concentration of the Hg<2+> according to the determined fluorescence intensity value. According to the method provided by the invention, fluorescence is intensively enhanced by self-assembling double-chain / G-quadruplex of the non-labeled DNA molecules induced by the Hg<2+>, and embedding ThT into double-chain DNA and G-quadruplex, so that a complicated DNA fluorescence labeling process is avoided and a fluorescence signal is dually amplified; the fluorescence analysis method is simple to operate, low in cost, good in specificity and high in sensitivity.

The invention relates to a pre-hydrated mineral powder nanocrystalline nucleus early strengthening agent for steam-curing concrete and a preparation method thereof. The early strengthening agent comprises, by mass, 20-30% of mineral powder, 2-3% of a surface modifier and 67-78% of deionized water. The surface modifier is a mixed solution containing a carboxylic acid-sulfonic acid polymer, a polycarboxylic acid water reducing agent and white sugar dissolved in water at a mass ratio of 1-3: 1-3: 1. The pre-hydrated mineral powder nanocrystalline nucleus early strengthening agent for steam-curingconcrete has strong induction effects on cement under steam-curing conditions. The early strengthening agent in a use ratio of 0.5-1.0% can improve the strength of ordinary Portland cement mortar by55-68% through steam-curing at 60 DEG C for 8h so that the early strength of steam-cured prefabricated components is significantly improved and the mold turnaround efficiency is improved. The early strengthening agent can be widely used in the steam-cured prefabricated component industry, greatly improve production efficiency and reduce energy consumption of the unit product.

A carrier board structure includes at least one upper magnetic coil, at least one lower magnetic coil, a flexible board, a dielectric layer, at least one connection pad and at least one gold finger. The flexible board has a middle region having a middle hole, and two side regions thinner than the middle region. A groove used as a fold line is provided on the lower surface of each side region bordering on the middle region. The upper and lower magnetic coils are configured in the flexible board and separated by the dielectric layer. The gold fingers are provided on the two side regions and connected to the upper magnetic coils. The upper and lower magnetic coils are around the middle hole and connected by the connection pads. The fold lines help the two side regions to fold without damage to the upper and lower magnetic coils.

The invention discloses a preparation method of a BETA-MOR symbiotic molecular sieve, which comprises the following steps: adding a BETA molecular sieve into an alkaline solution, stirring and mixing, and treating under sealed conditions to obtain a suspension product to serve as a structural guide agent; mixing inorganic alkali, aluminum source, silicon source and water according to a certain mol ratio to synthesize an initial silicon-aluminum gel; after evenly mixing, adding the structural guide agent; and after evenly mixing, carrying out two-step crystallization to obtain the BETA-MOR symbiotic molecular sieve. Compared with the process of directly adding the BETA molecular sieve crystal seed, the method disclosed by the invention greatly enhances the degree of crystallization of the BETA molecular sieve in the BETA-MOR symbiotic molecular sieve, and effectively avoids generation of stray crystals. The method disclosed by the invention is simple to operate and easy to implement, and the raw materials are cheap, accessible, nontoxic and harmless.

The invention relates to a front longitudinal beam energy-adsorption structure for a vehicle. The front longitudinal beam energy-adsorption structure comprises a front longitudinal beam assembly (10), wherein the front longitudinal beam assembly (10) comprises a body (1) and a cover plate (2); the body (1) has a U-shaped structure and is provided with a body upper wing (11), a body inside wing (12), a body lower wing (13) and a body lower flange (14); the cover plate (2) is provided with a cover plate upper flange (21) and a cover plate outside wing (22); one or more first L-shaped conduction structure (101) is arranged on the body (1); one side of each first L-shaped conduction structure (101) consists of convex structures (111) on the body upper wing; the other side of each first L-shaped conduction structure (101) consists of concave structures (121) on the body inside wing; and in order to match the first L-shaped conduction structures on the body (1), second L-shaped conduction structures (102) also can be arranged at corresponding positions on the cover plate (2). The front longitudinal beam energy-adsorption structure for the vehicle has a good mechanical property, good modeled processability and good welded processability.

The invention relates to a bioreactor for inducing three-dimensional directional differentiation in vitro of stem cells by virtue of non-contact coculture. The bioreactor is a container for cell culture; one or more than two separation meshes are arranged in the container so that the container is divided into more than two chambers; inducing cell embedded sodium alginate microcapsules and stem cell embedded calcium alginate microgel beads are arranged in different chambers, so that non-contact dynamic coculture of two or more than two types of cells under a three-dimensional growth condition in the same system is realized; the reactor is used for promoting the directional differentiation of the stem cells under the three-dimensional growth condition by virtue of an inducing factor secreted by the inducing cells in the microcapsules; after the completion of the differentiation, the microgel beads are dissolved by virtue of a sodium citrate solution, and finally, the differentiated cells can be obtained. The bioreactor is simple to operate and low in cost; the separation meshes in the bioreactor enable the microcapsules and the microgel beads to be separated from each other thoroughly, and then the two carriers are prevented from being mixed together.

Identified herein is the location of the MN protein binding site, and MN proteins / polypeptides that compete for attachment to vertebrate cells with immobilized MN protein. Such MN proteins / polypeptides prevent cell-cell adhesion and the formation of intercellular contacts. The MN protein binding site is a therapeutic target that can be blocked by organic or inorganic molecules, preferably organic molecules, more preferably proteins / polypeptides that specifically bind to that site. Therapeutic methods for inhibiting the growth of preneoplastic / neoplastic vertebrate cells that abnormally express MN protein are disclosed. Vectors are provided that encode the variable domains of MN-specific antibodies and a flexible linker polypeptide separating those domains. Further vectors are disclosed that encode a cytotoxic protein / polypeptide operatively linked to the MN gene promoter or a MN gene promoter fragment comprising the HIF-1 consensus binding sequence, and which vectors preferably further encode a cytokine. The MN gene promoter is characterized, and the binding site for a repressor of MN transcription is disclosed. Further, the hypoxia inducibility of the MN gene and the uses of such induciblity inducibility are disclosed.

The invention provides a Chinese cabbage isolated microspore inducing medium. According to the formula, the medium contains KNO3, MgSO4-7H2O, KH2PO4, Ca(NO3)2-4H2O, Na2-EDTA, FeSO4-7H2O, MnSO4-4H2O, AgNO3, H3BO3, ZnSO4-7H2O, KI, Na2MoO4-2H2O, CuSO4-5H2O, CoCl2-6H2O, L-glutamine, vitaminB1, vitaminB6, nicotinic acid, folic acid, biotin, inositol, glycine, proline, serine, L-glutathione, methylnitrosourea, sucrose, phytagel, 6-BA, complex peptide nucleic acid, protein hydrolysate, colchicines, activated carbon and plant vulcanized kinetin PSK-alpha. The medium can greatly improve the inducing efficiency of Chinese cabbage isolated microspore embryonic calluses and has important application value.

The invention discloses an induced respiration controller and mainly solves the problems that the prior respiration control technology is low in efficiency, inconvenient to implement and is high in cost. The induced respiration controller comprises an eyeball protection unit, a breathing lamp unit, a control unit and an electric power unit, wherein the eyeball protection unit is used for protecting eyeballs of a patient from being injured by radiation; the breathing lamp unit is arranged in the eyeball protection unit and is used for generating gradient light ray, enabling the eyeballs of the patient to sense the light and inducing the patient to regulate the breathing frequency and extent according to the light flicker frequency; the control unit is used for controlling and regulating the flicker frequency of the breathing lamp; and the electric power unit is used for supplying electric power to the breathing lamp and the control unit. Based on the structure, the invention also discloses an implementation method of the induced respiration controller. According to the induced respiration controller, the breathing speed and extent are controlled through lamplight, the speed of lamplight is adjusted in a visualized manner, the patient is enabled to adapt to the regulation more easily, the difficulty of respiratory training in clinical context is reduced, and the respiratory control efficiency is improved. Therefore, the induced respiration controller and the implementation method thereof are suitable for popularization and application.

The invention relates to a preparation method of a ginger extract with detoxifying effect on exogenous carcinogens and a ginger extract product, and belongs to the technical field of deep processing of ginger. The preparation method comprises the following steps of: selecting and cleaning fresh ginger serving as a raw material, performing freeze drying, crushing, extracting with ethanol solution,and performing concentration to obtain ginger extract; performing ethyl acetate / aqueous phase separation, collecting ethyl acetate layer, concentrating and drying, and thus obtaining the ethyl acetate extract; and finally, performing extraction by silica gel column chromatography, performing gradient elution by using ethyl acetate / n-hexane system or the like, collecting elution components of 30to 70 percent ethyl acetate eluent, performing vacuum concentration, volatilizing the residual solvent by using a nitrogen blowing instrument, packing and sealing, and thus obtaining the ginger extract. The extract can remarkably induce the activity of quinone oxidoreductase (QR) in rat liver cancer Hepa1c1c7 cells, shows good potential of up-regulated II phase detoxifying enzyme, and can be usedfor developing low-toxicity efficient products such as cancer preventing agent, functional food and the like.

The invention provides a free microspore induced culture medium for brassica campestris. A formula of the culture medium contains KNO3, MgSO4.7H2O, KH2PO4, Ca(NO3)2.4H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.4H2O, AgNO3, H3BO3, ZnSO4.7H2O, KI, Na2MoO4.2H2O, CuSO4 .5H2O, CoCl2.6H2O, chlorinated 2-hydroxyethyl trimethylamine, L-glutamine, vitamin B1, vitamin B6, nicotinic acid, folic acid, biotin, inositol, glycine, L-glutathione, methyl nitrosourea, sucrose, phytagel, 6-BA, NAA, polyethylene glycol, protein hydrolysate, active carbon and phytosulfokine PSK-alpha. The culture medium can be used for greatly improving the induction efficiency of free microspore embryogenic calli of brassica campestris, and has an important application value.

The invention discloses a method for improving induction effects of test-tube plantlets of potatoes. The method comprises the following steps: sequentially culturing embedding beads of potatoes with bud stems by using six culture media containing graphene quantum dots and carbon nano tubes, and forming many large test-tube plantlets within 12 days after culture. Compared with the prior art, the method disclosed by the invention has the advantages that the test-tube potatoes formed from the test-tube plantlets of potatoes are large in quantity, large in diameter and excellent in effect.

The invention discloses an emergency guiding linkage system for an urban rail transit. The emergency guiding linkage system for the urban rail transit comprises an automatic fire alarming system, a linkage controller, a main controller, an emergency evacuation strobe light, an audible and visual alarm and an operation dispatching workstation, wherein the automatic fire alarming system is used foracquiring fire position information in a subway station; the linkage controller is used for reading the fire position information; the main controller is used for receiving the fire position information; the emergency evacuation strobe light is connected with the main controller; the audible and visual alarm is arranged at a passageway of the subway station, and is connected with the main controller; the operation dispatching workstation is connected with the main controller and is used for inputting emergency level marks; the main controller controls the emergency evacuation strobe light to indicate an evacuation direction and controls the audible and visual alarm to send out audible and visual alarming according to the fire position information or emergency level marks to guide passengers in the subway station to evacuate out of the station. By the emergency guiding linkage system for the urban rail transit, boundedness of a single guiding means can be avoided, meanwhile, automated systems of the subway station can be linked to guide and cooperate with evacuation of the passengers, and the passenger evacuation efficiency is improved.

The invention relates to a preparation method of a hydroxyapatite / drug-loaded hydrogel composite coating on the surface of micro-arc titanium oxide. The preparation method comprises the following steps of: soaking a titanium sample subjected to micro-arc oxidation treatment in an alkaline solution, and carrying out hydrothermal treatment to form a hydroxyapatite bioactive coating on the surface of micro-arc titanium oxide; putting an aluminum template into a culture dish, then adding a mixture of a prepolymer of PDMS and a curing agent, and curing to obtain a PDMS template; and mixing a chitosan aqueous solution and a sodium alginate aqueous solution, adding a drug to obtain a drug-loaded hydrogel solution, adding a photoinitiator to the drug-loaded hydrogel solution, dropwise adding to the template, placing a sample on the PDMS template, curing under ultraviolet light, and carrying out freeze drying. According to the method, a composite coating with a porous structure and a nanorod shape is generated through micro-arc oxidation and hydrothermal treatment, and the bonding strength and the biological activity of the implant and bones are improved. The drug-loaded composite hydrogel is loaded on the surface of the micro-arc titanium oxide through the PDMS template, so that the implant coating is endowed with more biological functions.

The invention relates to a mobile reciting learning aid for teaching, which adopts mobile recitation for the memorization of knowledge points and solves the problems that recited answers come into the sight of a reciter to influence recitation effect and that the reciter looks for the answers to cause time waste and false memory in the prior art. The mobile reciting learning aid comprises a questioning outline printed page (1) and an answer-filling printed page (2) which are superposed, wherein the surface of the questioning outline printed page (1) is provided with a plurality of hollowed holes (1-1); the upper surface of the answer-filling printed page (2) is printed with a plurality of text blocks; the plurality of hollowed holes correspond to the plurality of text blocks one to one; and the lengths and heights of each text block are the same as those of the corresponding hollowed holes respectively.