Preparation method of three-layer lead tissue regenerating velum with gradient

A technique of guiding tissue regeneration and gradient, applied in the field of preparation of three-layer guided tissue regeneration membrane, can solve the problems of poor mechanical strength, affecting tissue regeneration, membrane collapse, etc., and achieve the advantages of repairing regeneration and increasing dust compatibility Effect

Inactive Publication Date: 2009-11-25
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although natural biofilm materials have excellent biocompatibility, they degrade quickly and have poor mechanical strength, which can easily lead to membrane collapse and affect tissue regeneration; although synthetic polymer membrane materials are relatively inferior to collagen in biological properties, their degradation speed is relatively low. Slow, but has good mechanical properties and thermal stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Dissolve different amounts of gelatin in trifluoroethanol to obtain solutions A, B, and C with gelatin concentrations of 3%, 5%, and 10%, respectively, and stir magnetically for 12 hours.

[0029] 2. Add different amounts of polylactic acid and nano-hydroxyapatite to solutions A, B and C respectively to obtain solutions D, E and F. The contents of polylactic acid in D, E and F are 10%, 5% and 3% respectively. %, the concentration of nano-hydroxyapatite is 0.005%, and the D, E and F solutions are magnetically stirred for 12 hours.

[0030] 3. Take solution E (3mL) for electrospinning, use aluminum foil as the receiver, the electrospinning voltage is 10kv, the receiving distance is 12cm, and the flow rate is 0.2mL / h to obtain the first layer of film;

[0031] 4. Take solution D (10mL) and continue electrospinning on the first layer of membrane material, using aluminum foil as the receiver, the electrospinning voltage is 10kv, the receiving distance is 12cm, and the flo...

Embodiment 2

[0036] 1. Dissolve different amounts of collagen in hexafluoroisopropanol to obtain solutions A, B, and C with gelatin concentrations of 1%, 3%, and 8%, respectively, and stir magnetically for 8 hours.

[0037] 2. Add different amounts of polycaprolactone and nano-hydroxyapatite into solutions A, B and C respectively to obtain solutions D, E and F. The contents of polycaprolactone in D, E and F are respectively 10%, 6% and 2%, the concentration of nano-hydroxyapatite is 0.1%, respectively, D, E and F solutions are magnetically stirred for 20 hours.

[0038] 3. Take solution E (3mL) for electrospinning, use aluminum foil as the receiver, the electrospinning voltage is 8kv, the receiving distance is 18cm, and the flow rate is 1mL / h to obtain the first layer of film;

[0039] 4. Take solution D (5mL) and continue electrospinning on the first layer of membrane material, using aluminum foil as the receiver, the electrospinning voltage is 13kv, the receiving distance is 10cm, and th...

Embodiment 3

[0044] 1. Dissolve different amounts of collagen in hexafluoroisopropanol to obtain solutions A, B, and C with gelatin concentrations of 3%, 8%, and 12%, respectively, and stir magnetically for 24 hours.

[0045]2. Add different amounts of polylactic acid and nano-hydroxyapatite to solutions A, B and C respectively to obtain solutions D, E and F. The contents of polylactic acid in D, E and F are 7%, 6% and 2%, respectively. %, the concentrations of nano-hydroxyapatite were 1%, and the D, E and F solutions were magnetically stirred for 10 h.

[0046] 3. Take solution E (3mL) for electrospinning, use aluminum foil as the receiver, the electrospinning voltage is 20kv, the receiving distance is 11cm, and the flow rate is 1mL / h to obtain the first layer of membrane material;

[0047] 4. Take solution D (12mL) and continue electrospinning on the first layer of membrane material, using aluminum foil as the receiver, the electrospinning voltage is 10kv, the receiving distance is 12cm,...

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PUM

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Abstract

The invention relates to a preparation method of three-layer lead tissue regenerating velum with gradient, belonging to macromolecule biomedical material field. The velum is a three-layer composite velum made from natural biological material, degradable polymer and nanometer hydroxyapatite as raw materials. The method comprises steps of dissolving mixture of the natural biological material, degradable polymer and nanometer hydroxyapatite with different proportions in a special solvent; preparing the natural biological material / degradable polymer / nanometer hydroxyapatite three-layer gradient composite velum through static spinning layer by layer; and using pure natural biological material to modify surface of the velum by means of static self-assembly layer by layer. The velum has excellentbiological compatibility and mechanical property; one side of the velum is crude and loose, so the side can face to a defect area and is helpful for bones to absorb and grow and for blood coagulationblocks to stabilize; the other side is smooth and compact, so the side can face to surrounding tissue to prevent connective tissues from entering the defect area.

Description

technical field [0001] The invention belongs to the field of macromolecule biomedical materials, and in particular relates to a preparation method of a gradient three-layer guide tissue regeneration membrane. Background technique [0002] The guided tissue regeneration membrane was originally applied in the research of periodontal tissue repair and regeneration, using the physical barrier function of the membrane to isolate the defect tissue from the surrounding tissue, so as to form and maintain a gap that is conducive to tissue regeneration, and prevent connective tissue and epithelial cells from entering the defect Areas that promote cell growth, proliferation, and differentiation to form new tissues. [0003] Guided tissue regeneration Membrane materials can be divided into absorbable and non-absorbable materials, both of which have the function of blocking cells and guiding specific tissue regeneration. Non-absorbable membrane materials represented by polytetrafluoroet...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/58A61L27/56A61L27/12A61L27/24A61L27/22A61L27/20A61L27/18A61L27/16
Inventor 任天斌曹春红袁伟忠宋莹冯玥任杰
Owner TONGJI UNIV
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