Bio-active 3-D porous tissue engineering support material and its preparation method

A three-dimensional porous, tissue engineering technology, applied in medical science, surgery, etc., can solve the problems that materials cannot achieve satisfactory results, destroy the natural conformation of growth factors, lose the activity of growth factors, etc., to broaden the selection range and control the conditions. , good biocompatibility

Inactive Publication Date: 2004-02-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since growth factors are easily inactivated in aqueous solution, the activity of growth factors is often greatly lost during the processing of materials; although the freeze-drying adsorption method can better maintain the activity of growth factors, it is only suitable for water-soluble The preparation of porous materials, and water-soluble materials are not suitable for use as scaffold materials; the chemical grafting method will destroy the natural conformation of growth factors, and also cause the final result of inactivation of growth factors
Therefore, the current various methods of compounding factors into materials, as well as the prepared materials, cannot achieve satisfactory results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The fine powder PLA (Mn=5×10 4 ) 0.200g and 0.060g of chitosan and its derivatives are ground and mixed evenly in agate grind pottery, placed in a square mold container, and then the mold is placed on SC-CO 2 In the 0.50L reactor of the equipment, process for 120min under the conditions of 20MPa and 35°C, take out the sample, and obtain the PLA / chitosan composite three-dimensional porous scaffold material in pale yellow square shape. The mechanical properties of the material were tested by a universal tensile machine, and the results showed that its mechanical strength was significantly higher than that of the pure PLA scaffold material.

Embodiment 2

[0031] The fine powder PLA (Mn=8×10 4 ) 0.200g, chitosan and its derivatives 0.040g, and lyophilized powder containing active growth factor 0.020g (containing basic fibroblast growth factor (bFGF) 0.1mg, heparin 0.1mg, bovine serum albumin (BSA) )0.020g) Grind and mix evenly in agate porridge, put it in a cylindrical mold container, and then place the mold on SC-CO 2 Seal the 0.5L reactor of the equipment. Pump in CO frozen into liquid 2 In the kettle, make the pressure in the kettle reach 30Mpa, adjust the temperature in the kettle to 32℃, slowly release the pressure after 10 minutes, open the kettle body, take out the sample, and obtain the light yellow plate-like PLA / chitosan / bFGF composite activity Three-dimensional porous scaffold material.

Embodiment 3

[0033] Put PLA(Mn=1×10 5 ) 0.400g transparent film, folded into several layers in the direction of the midline, and then mix the uniformly mixed chitosan powder 0.080g and active growth factor-containing freeze-dried powder 0.010g (containing nerve growth factor (NGF) 0.05mg, Heparin 0.05mg, bovine serum albumin (BSA) 0.010g) are evenly sprinkled on the surface of each layer of membrane, then the folded membrane is rolled into a thin tube with the needle tube as the center, and then placed in supercritical CO 2 In the reaction kettle, treat for 60 min under the conditions of 10MPa and 35°C, take out the sample, and withdraw the needle tube to obtain a pale yellow cylindrical PLA / chitosan / NGF tubular composite active three-dimensional porous scaffold material. The activity test showed that the active NGF released by the material accounted for 70% of the coating volume, which was significantly higher than the traditional coating method.

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PUM

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Abstract

The present invention relates to a bio-active 3-D porous tissue engineering material and its preparation method. It uses the polylactate or polyethylene-propylene lectide, chitosan and its derivative, bovine serum albumin, growth factor and heparin as raw material, and adopts the supercritical CO2 fluid technology to prepare 3-D porous supporting material which can continuously release active growth factor. It has good mechanical property and biological compatibility.

Description

Technical field [0001] The invention relates to a bioactive three-dimensional porous tissue engineering scaffold material and a preparation method thereof, and more specifically to poly(DL-lactide, PLA) and poly(DL-lactide-co-glycolide) , PLGA), compound chitosan and its derivatives, growth factors (BoneMorphogenetic Proteins, BMPs; basic Fibroblast Growth Factor, bFGF; acid Fibroblast Growth Factor, aFGF; Epidermal growth factor, EGF; Nervegrowth factor, NGF) ternary organisms Active three-dimensional porous tissue engineering scaffold materials and their use of supercritical carbon dioxide fluid technology (SC-CO) 2 -FT) new preparation technology. Background technique [0002] The preparation of degradable materials with a three-dimensional porous scaffold structure that is conducive to tissue cell growth, reproduction and organ function recovery is a new research field in tissue engineering in recent years. At present, the methods used to prepare p...

Claims

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Application Information

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IPC IPC(8): A61L31/04
Inventor 周长忍张润李立华
Owner JINAN UNIVERSITY
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