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Preparation method and product of ginger extract with detoxification effect on exogenous carcinogens

A technology of ginger extract and detoxification, which is applied in the field of ginger deep processing, can solve the problems of low comprehensive utilization rate, backward ginger deep processing technology, and few types of ginger processing, and achieve the effect of simple preparation process

Active Publication Date: 2011-12-07
NANJING UNIV OF FINANCE & ECONOMICS +1
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Problems solved by technology

[0005] Ginger is a traditional medicinal and edible plant. my country has abundant ginger resources and a long history of planting. However, due to the low level of ginger processing technology in my country, especially the relatively backward ginger deep processing technology, there are currently few ginger processing varieties, low technical added value, Comprehensive utilization rate is low
Although ginger and its extracts have shown a wide range of biological activities such as anti-inflammation, anti-emetic, anti-tumor and antibacterial, there are no reports and patents related to the cancer prevention effect of ginger by regulating phase II metabolic enzymes.

Method used

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  • Preparation method and product of ginger extract with detoxification effect on exogenous carcinogens

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Effect test

Embodiment 1

[0012] Embodiment 1: the preparation of ginger extract

[0013] 2.21kg fresh ginger (Zingiber officinale Rosc.) was purchased in a local supermarket in April 2009. After being selected, wash and remove mud, freeze-dry, and then pulverize, extract the above-mentioned ginger powder with 95% ethanol solution (1:10, m / v) at 70 ° C for 4 hours, filter and take the extract to concentrate in vacuum rotation . Dissolve the alcoholic extract of ginger in 500mL of water, then add an equal volume of ethyl acetate, let stand to separate layers, collect the ethyl acetate layer, extract repeatedly 5-6 times, combine the extracts and concentrate and dry; take 10g of ginger ethyl acetate extract Mix with 3 times the mass of silica gel, use a 5.5cm×60cm glass column for silica gel column chromatography, and perform isocratic elution with ethyl acetate / n-hexane (0-100% ethyl acetate, v / v) system, Collect 30%-70% ethyl acetate eluate elution fraction, concentrate in vacuum rotation, evaporat...

Embodiment 2

[0014] Example 2: Determination of Phase II Enzyme Activity Induced by Ginger Extract

[0015] 2.1 Principle: quinone oxidoreductase (QR) is an important phase II detoxification enzyme. It has the advantages of easy synergy with other phase II enzymes, good responsiveness to elicitors, and convenient measurement, and is widely used as a biomarker for the determination of phase II enzymes; while the expression of QR in the mouse liver cancer Hepa 1c1c7 cell line is stable and reproducible The good performance makes this cell line gradually become a fast and reliable model for the determination of QR enzyme activity.

[0016] 2.2 Cell line: mouse liver cancer hepa1c1c7 cells, purchased from American Type Culture Collection (ATCC).

[0017] 2.3 Determination method: Hepa1c1c7 cells with a confluence of 80% were seeded in 96-well plate at 5000 / well, 37°C, 5% CO 2 cultured in an incubator for 24 h. Then add 200 μL of fresh culture solution containing ginger extract to each wel...

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Abstract

The invention relates to a preparation method of a ginger extract with detoxifying effect on exogenous carcinogens and a ginger extract product, and belongs to the technical field of deep processing of ginger. The preparation method comprises the following steps of: selecting and cleaning fresh ginger serving as a raw material, performing freeze drying, crushing, extracting with ethanol solution,and performing concentration to obtain ginger extract; performing ethyl acetate / aqueous phase separation, collecting ethyl acetate layer, concentrating and drying, and thus obtaining the ethyl acetate extract; and finally, performing extraction by silica gel column chromatography, performing gradient elution by using ethyl acetate / n-hexane system or the like, collecting elution components of 30to 70 percent ethyl acetate eluent, performing vacuum concentration, volatilizing the residual solvent by using a nitrogen blowing instrument, packing and sealing, and thus obtaining the ginger extract. The extract can remarkably induce the activity of quinone oxidoreductase (QR) in rat liver cancer Hepa1c1c7 cells, shows good potential of up-regulated II phase detoxifying enzyme, and can be usedfor developing low-toxicity efficient products such as cancer preventing agent, functional food and the like.

Description

1. Technical field [0001] The invention relates to a preparation method of a ginger extract capable of detoxifying exogenous carcinogens and a product thereof, which belongs to the technical field of ginger deep processing and can be used to develop low-toxicity and high-efficiency cancer preventives, functional foods and other products. 2. Background technology [0002] With the development of social production and the acceleration of industrialization, people have more and more opportunities to be exposed to various chemical poisons in daily life and production. According to statistics, there are more than 30 million registered chemicals in the world, of which 80,000 to 100,000 are commonly used. These exogenous chemicals mainly include: industrial pollutants, environmental pollutants, harmful ingredients in food (natural toxins and additives), agricultural chemicals and radionuclides, etc., which can pass through different routes (contact, respiratory tract, digestive tra...

Claims

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Application Information

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IPC IPC(8): A61K36/9068A61P39/02A61K125/00
Inventor 胡秋辉李锋辛志宏方勇安辛欣杨方美马宁
Owner NANJING UNIV OF FINANCE & ECONOMICS
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