30 results about "Quinone oxidoreductase" patented technology

Disclosed herein are various bladder cancer treatments and methods. The present disclosure can take advantage of propylene glycol concentrations and / or NAD(P)H:quinone oxidoreductase-1 (NQO1), Cytochrome P450 Oxidoreductase (P450R) and Glucose transporter 1 (Glut-1) protein expression in human transitional cell carcinoma of the bladder to offer individually targeted bladder cancer treatments.

The present disclosure pertains to a genetically modified microorganism that satisfies some of specific requirements. The specific requirements include: (I) compared to a wild type microorganism, having reduced or inactivated succinate dehydrogenase activity or fumarate reductase activity; (II) compared to a wild type microorganism, having reduced or inactivated lactate dehydrogenase activity; (III) having a modified phosphoenolpyruvate carboxylase activity and thus showing resistance against the feedback inhibition by aspartic acid in wild type phosphoenolpyruvate carboxylase activity, or having an exogenous phosphoenolpyruvate carboxylase activity and thus showing higher resistance against the feedback inhibition by aspartic acid than wild type phosphoenolpyruvate carboxylase activity shown by a wild type microorganism; and (IV) compared to a wild type microorganism, having reduced or inactivated pyruvate : quinone oxidoreductase.

Provided is a method capable of effectively treating various diseases associated with energy excess, such as obesity, diabetes, metabolic syndromes, degenerative diseases and mitochondrial dysfunction-related diseases, via elevation of an NAD(P)+ / NAD(P)H ratio by increasing an NAD(P)+ concentration in vivo or in vitro through use of NAD(P)H as a substrate or coenzyme by oxidoreductase such as NAD(P)H:quinone oxidoreductase (NQO1), a method of screening a drug for the same and a therapeutic drug.

The invention discloses an immobilized heterotrophic microorganism for removing sulfides. The immobilized heterotrophic microorganism is prepared by wrapping a heterotrophic microorganism of which a genome comprises a sulfur quinone oxidoreductase gene and a sulfide dioxygenase gene in alginic acid latex spheres with a support function, and meanwhile adding a set amount of fe3o4 nanoparticles. The invention further discloses application of the immobilized heterotrophic microorganism in removing sulfides in wastewater or waste gases under an aerobic reaction condition. Tests show that introduction of sulfides and nutritive substances is not blocked by the cell immobilization method disclosed by the invention, heterotrophic microorganisms can be bred in the sulfides and the nutritive substances, and thus the sulfide oxidation efficiency of the immobilized cells of a unit volume is improved in the culture process; in addition, acidification problems are avoided in the sulfide removal process, conventional difficulties of bioremediation of sulfides are effectively solved, and the immobilized heterotrophic microorganism has the characteristics of repeated use, low culture cost, high sulfide endurance and the like.

The invention discloses a method for removing hydrogen sulfide by utilizing heterotrophic microorganisms. Specific heterotrophic microorganisms are added in a device for removing hydrogen sulfide, an aerobic reaction is performed in a set reaction system, and removal on H2S, HS- or / and S2- in the reaction system of the device is implemented, wherein the heterotrophic microorganisms mean a category of heterotrophic bacteria of which a genome comprises a sulfide:quinone oxidoreductase gene and a persulfid dioxygenase gene and which can oxidize sulfides to generate zero-valent sulphur and thiosulfate, or sulfate. According to the method disclosed by the invention, the screened heterotrophic bacteria have efficient capacity of oxidize exogenous hydrogen sulfide, has the characteristics of high growth speed, low culture cost and capacity of forming great biomass, effectively solves the problem of condition limitation in the prior art in which autotrophic bacteria are used for remove hydrogen sulfide, and has a great application potential in the industrial production.

Disclosed herein are various bladder cancer treatments and methods. The present disclosure can take advantage of propylene glycol concentrations and / or NAD(P)H:quinone oxidoreductase-1 (NQO1), Cytochrome P450 Oxidoreductase (P450R) and Glucose transporter 1 (Glut-1) protein expression in human transitional cell carcinoma of the bladder to offer individually targeted bladder cancer treatments.

The invention provides a biomimetic nano-carrier with dual functions of oxidation and anti-oxidation, a preparation method and application thereof. The biomimetic nano-carrier provided by the invention comprises red blood cell membrane and an organometallic framework coated in the red blood cell membrane, and the organometallic framework is loaded with functionalized SOD enzyme and β-lapachone. After the biomimetic nanocarrier reaches the acidic environment of tumor cells, the metal-ligand bond in the MOF is hydrolyzed to produce protonated ligands that destroy the red blood cell membrane, and the enzyme and drug are released; β-lapachone is oxidized and reduced by the quinone highly expressed in tumor cells Enzyme 1 catalyzes the production of a large amount of superoxide anion, which is catalyzed by SOD enzyme to produce hydrogen peroxide. Finally, iron ions in SOD enzyme catalyze hydrogen peroxide to produce hydroxyl free radicals through Fenton reaction, thereby killing tumor cells. The biomimetic nano-carrier provided by the invention not only does not produce drug resistance through enzyme treatment, but also can significantly reduce systemic toxicity caused by drug administration, and has a very high clinical application prospect.