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Application of tangeretin in preparation of products to activate ability of cells to resist oxidative stress

A technology of oxidative stress and orange peel, applied in the application field of the product, to achieve the effect of maintaining the ability to resist oxidative stress

Inactive Publication Date: 2019-09-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the improvement of tangeretin and the maintenance of the ability of cells to resist oxidative stress damage, especially the research on increasing the ability of L02 cells to resist oxidative stress by activating the oxidative stress pathway.

Method used

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  • Application of tangeretin in preparation of products to activate ability of cells to resist oxidative stress
  • Application of tangeretin in preparation of products to activate ability of cells to resist oxidative stress
  • Application of tangeretin in preparation of products to activate ability of cells to resist oxidative stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Effect of Tangeretin on Intracellular Active Oxygen Content in Human Liver L02 Cells Suffering Hydrogen Peroxide Oxidative Stress Damage

[0023] Human liver L02 cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37°C, 5% CO 2 Incubate in a cell culture incubator, replace with fresh culture medium every other day, and passage once every 2-3 days. During the experiment, L02 cells were seeded in 6-well plates. When the cells grow to 70-80% confluence, the original medium is discarded, and the drugs are added in groups. A blank control group (without hydrogen peroxide treatment), a DMSO solvent control group and a tangeretin treatment group (final concentration of 2 μmol / L) were set up. After acting for 6 hours, keep (or remove) the medium containing tangeretin, and add a serum-free medium containing hydrogen peroxide at a concentration of 0.78mmol / L. After incubation for 30 min, the original medium was removed, washed twice with PBS, and ...

Embodiment 2

[0025] Example 2 Effect of Tangeretin on Intracellular Antioxidative Enzyme Activity of Human Liver L02 Cells

[0026] Human liver L02 cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37°C, 5% CO 2 Incubate in a cell culture incubator, replace with fresh culture medium every other day, and passage once every 2-3 days. During the experiment, L02 cells were seeded in 96-well plates. When the cells grow to 70-80% confluence, the original medium is discarded, and the drugs are added in groups. Set up DMSO solvent control group and different concentrations of tangeretin treatment groups (final concentrations were 0.5 μmol / L, 1 μmol / L, 2 μmol / L, respectively). After acting for 6 hours, the culture medium containing tangeretin was retained (or removed). Biyuntian catalase detection kit (S0051) was used to detect the activity of catalase (CAT). The enzyme activity of quinone oxidoreductase (NQO1) was detected by Abcam quinone oxidoreductase detection kit (ab...

Embodiment 3

[0028] Example 3 Effect of Tangeretin on Catalase (CAT) and Quinone Oxidoreductase (NQO1) Protein Expression in Human Liver L02 Cells

[0029] Human liver L02 cells were cultured with RPMI-1640 containing 10% fetal bovine serum at 37°C, 5% CO 2Incubate in a cell culture incubator, replace with fresh culture medium every other day, and passage once every 2-3 days. During the experiment, L02 cells were seeded in 6-well plates. When the cells grow to 70-80% confluence, the original medium is discarded, and the drugs are added in groups. A DMSO solvent control group and a tangeretin treatment group (final concentration of 2 μmol / L) were set up. After acting for 6 hours, the cells were lysed with NP40 lysate containing protease inhibitors and protein phosphatase inhibitors, and the total protein was collected. The protein concentration was determined by the BCA method, and the protein was separated by SDS-PAGE gel and transferred to PVDF membrane. ACTIN was used as a reference, ...

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Abstract

The invention provides application of tangeretin in the preparation of products to activate ability of cells to resist oxidative stress. The products include functional foods, health products or drugs. Tangeretin can improve evidently the ability of human liver L02 cells to resist hydrogen peroxide oxidative stress injury, can enhance the expression of antioxidase quinone oxidoreductase NQO1 and catalase CAT by adjusting oxidative stress-related Nrf2-Keap1-Cul3 pathway, and can inhibit Ubiquitylation degradation of Nrf2 by inhibiting the expression of Cul3 so as to maintain the ability of L02 cells to resist oxidative stress. Therefore, tangeretin can act as a functional food, health product or preventive drug to prevent and treat hepatic oxidative stress injury related diseases.

Description

technical field [0001] The invention belongs to the field of food and medicine, and relates to the application of tangeretin in the preparation of products with oxidative stress function, in particular to the application of tangeretin in the preparation of products for activating the ability of cells to resist oxidative stress. It is the application of tangeretin in the resistance of human liver L02 cells to hydrogen peroxide oxidative stress. Background technique [0002] Oxidation and reduction are the most common chemical reactions in nature, manifested as electron gain or loss or shift of covalent electron pairs. Oxidative stress damage occurs when the accumulation rate of oxides in the organism exceeds the reduction reaction and the removal rate of oxides in the organism. Studies have shown that oxidative stress injury is associated with the occurrence and development of various diseases, including cancer, diabetes, obesity, nervous system diseases and atherosclerosis....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/10A61K31/352A61P39/06
CPCA23L33/10A61K31/352A61P39/06
Inventor 孙崇德王岳曹锦萍李鲜陈昆松
Owner ZHEJIANG UNIV
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