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A kind of immobilized heterotrophic microorganism for removing sulfide and its preparation method and application

A microorganism and sulfide technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as unreported reports, and achieve the effect of increasing specific surface area

Active Publication Date: 2020-11-20
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the search found that the fixation in the Fe-containing 3 o 4 Heterotrophic microorganisms in alginic acid colloids of nanoparticles, an immobilized Fe-containing microbe for the removal of sulfide 3 o 4 Heterotrophic microorganisms in alginic acid colloidal spheres of nanoparticles and the patents or related literatures of their preparation methods and applications have not yet been reported

Method used

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  • A kind of immobilized heterotrophic microorganism for removing sulfide and its preparation method and application
  • A kind of immobilized heterotrophic microorganism for removing sulfide and its preparation method and application
  • A kind of immobilized heterotrophic microorganism for removing sulfide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Preparation of immobilized cells of Gluconobacter oxydans 621H and Pseudomonasputida S16.

[0041] (1) Pick single colonies of Gluconobacter oxydans 621H or Pseudomonas putida S16 on the plate and put them into test tubes respectively, and use rich medium to activate and culture at 30 degrees Celsius and 200 rpm for more than 24 hours , and then transferred to shake flasks to expand the culture according to the volume ratio of 1:100 until the turbidity of the bacterial solution was OD 600 More than 3, collect the cells by centrifugation.

[0042] in:

[0043] The formula of the rich medium is: 30 g / L of glucose for industrial fermentation, and 15 g / L of dry corn steep liquor powder for industrial fermentation.

[0044] (2) Resuspend the cells collected in step (1) with distilled water, and add sodium alginate aqueous solution to make the wt / vol concentration of sodium alginate 3%, so that the ratio of the wet weight of the cells to the dry weight of the sodium alginat...

Embodiment 2

[0046] The application of the immobilized heterotrophic microorganisms for removing sulfides obtained in Example 1 for removing sulfides in wastewater or waste gas under aerobic reaction conditions.

[0047] The application method is a conventional known method; wherein, the aerobic reaction conditions and the reaction system are: pH 5-9, the oxygen content is greater than 2mg / L, or the shaking rate is 150±50r.p.m.; the reaction system is a buffer that is harmless to bacteria solution (such as: HEPES buffer) or medium, wherein the system is also added with sodium hydrosulfide at a concentration of 2-2000 μM.

Embodiment 3

[0049] Gluconobacter oxydans 621H or Pseudomonasputida S16 immobilized cells, added Fe 3 o 4 Comparison of Sulfide Oxidation Rates by Immobilized and Free Cells of Nanoparticles.

[0050] Based on the method disclosed in Example 1, the immobilized cells of Gluconobacteroxydans 621H or Pseudomonas putida S16 were prepared respectively, and Fe 3 o 4 Immobilized cells and free cells with nanoparticles.

[0051] will contain Fe 3 o 4 Nanoparticle or Fe-free 3 o 4 The immobilized cells of the nanoparticles or the same amount of free cells were suspended in an equal amount of HEPES buffer, and then freshly prepared NaHS was added to a final concentration of 2000 μM to start the reaction, sealed, and the entire reaction condition was 30°C with slight shaking (100± 10r.p.m.), and at 5 minutes, 10 minutes, and 20 minutes, three time points were taken to measure the remaining concentration of hydrogen sulfide in the system, so as to compare the differences in the oxidation rates ...

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Abstract

The invention discloses an immobilized heterotrophic microorganism for removing sulfides. The immobilized heterotrophic microorganism is prepared by wrapping a heterotrophic microorganism of which a genome comprises a sulfur quinone oxidoreductase gene and a sulfide dioxygenase gene in alginic acid latex spheres with a support function, and meanwhile adding a set amount of fe3o4 nanoparticles. The invention further discloses application of the immobilized heterotrophic microorganism in removing sulfides in wastewater or waste gases under an aerobic reaction condition. Tests show that introduction of sulfides and nutritive substances is not blocked by the cell immobilization method disclosed by the invention, heterotrophic microorganisms can be bred in the sulfides and the nutritive substances, and thus the sulfide oxidation efficiency of the immobilized cells of a unit volume is improved in the culture process; in addition, acidification problems are avoided in the sulfide removal process, conventional difficulties of bioremediation of sulfides are effectively solved, and the immobilized heterotrophic microorganism has the characteristics of repeated use, low culture cost, high sulfide endurance and the like.

Description

technical field [0001] The invention relates to a product for removing sulfides and its preparation method and application, in particular to a product for removing sulfides fixed on Fe-containing 3 o 4 Heterotrophic microorganisms in alginic acid gel balls of nanoparticles and its preparation method and application. Background technique [0002] Hydrogen sulfide is a poisonous gas with a pungent smell, and is the main waste gas component widely existing in industrial production processes (such as sewage treatment plants, animal husbandry and composting plants, etc.). At present, the removal methods of harmful hydrogen sulfide gas are mainly divided into physical methods, chemical methods and biological methods. Among them, biological methods are cheap and more environmentally friendly to remove hydrogen sulfide, and are currently widely used. [0003] In the process of biological removal of hydrogen sulfide, microorganisms play a vital role in the oxidation and absorption ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10C12N11/14C12N1/21C02F3/34B01D53/84B01D53/48C12R1/01C12R1/40
CPCB01D53/48B01D53/84C02F3/34C02F2101/101C12N9/0004C12N9/0067C12N11/10C12N11/14C12Y112/05Y02A50/20
Inventor 荀鲁盈侯宁可刘红蕾
Owner SHANDONG UNIV
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