139 results about "Gluconobacter" patented technology

The invention relates to a method for producing bacterial cellulose with wheat straws / maize straws, comprising the following steps: (1) grinding the wheat straws or maize straws, soaking the ground wheat straws or maize straws in dilute sulfuric acid or hydrochloric acid to react, then separating the residues of the wheat straws or maize straws from the acid hydrolysate through pump filtration and collecting the hydrolysate for later use; (2) detoxicating the hydrolysate; and (3) taking the detoxicated hydrolysate as the carbon source in the culture medium and adding the nitrogen source to prepare the culture medium and inoculating acetobacter aceti or gluconobacter oxydans into a shaker at 25-30 DEG C and 160-250r / min to be cultured or an incubator at 25-30 DEG C to undergo static culture, thus obtaining the bacterial cellulose. The carbon source in the culture medium is good in quality, low in price and suitable for industrial production.

The invention relates to a strain HD924 for producing dihydroxyacetone by microbial fermentation. The strain HD924 is classified into Acetobacteraceae Gluconobacter Gluconobacterfrateurii, and its collection number in the common micro-organism center of the CCCCM is CGMCC No.5397. The invention also provides a method for producing dihydroxyacetone by using the strain to ferment glycerin. dihydroxyacetone can be remarkably accumulated under aerobic conditions with the maximum of 170g / L. By the adoption of the method provided by the invention, industrial production can be realized.

The invention discloses a method for improving gluconobacter oxydans for producing 2-keto-L-gulconic acid, and belongs to the technical field of genetic engineering. On the basis of genetic engineering transformation, adaptor protein SH3 and ligand protein SH3lig of the adaptor protein, a CutA gene and a key enzyme gene in saccharic acid transformation are subjected to fusion expression, and G.oxydans is transformed to produce 2-KLG in a one-step fermentation manner, so that the yield of 2-KLG is finally increased to be 40.3 g / L, that is, the yield is increased by 24.4% when being compared with that of a one-step engineering bacterium of which CutA is not expressed; in addition, due to the thermal resistance of foreign protein, the thermal resistance of the engineering bacterium is further improved. Enzyme cross-linking inside cells can be achieved by expressing the foreign protein CutA, continuous catalytic reaction of a plurality of enzymes can be facilitated, and the method has significance in is one-step fermentation production of vitamin C.

In order to provide a method for culturing an immunopotentiator-containing organism having the experience of being eaten, inexpensively without requiring usage of a component derived from an animal, Acetobacter, Gluconobacter, Xanthomonas, Zymomonas or Enterobacter which is an edible gram-negative bacterium having an immunopotentiation function is cultured using a culture solution composed mainly of wheat or bean curd refuse. Thereby, Acetobacter can be obtained inexpensively and safely, and also a low molecular weight lipopolysaccharide which is the immunopotentiator can be obtained inexpensively and safely. Furthermore, no impurity derived from animal components is mixed.

The invention relates to a method for preparing bacterial cellulose by using bagasse. The method comprises steps of: (1) drying and crushing the bagasse, soaking the bagasse in acid with a solid-liquid ratio of 1:5-1:20, then reacting at 100 DEG C-240 DEG C for 50-90 min, filtering after the reaction, and collecting a hydrolysate; (2) detoxifying the hydrolysate; and (3) using the detoxified hydrolysate as a carbon source for a medium, adding 0.1-2% of a nitrogen source, and sterilizing at 121 DEG C for 15-20 min to obtain a culture medium; inoculating bacillus aceticus or Gluconobacter oxydans with an inoculation amount of 5%-10%; and conducting shaking culture at 25-30 DEG C with 160-250rpm or conducting static culture at 25-30 DEG C for 6-25 days, so as to obtain bacterial cellulose. The culture medium carbon source prepared by the invention has good quality and low price, and is suitable for industrialized production.

This invention relates to an novel Gluconobacter oxydans 2-ketoreductase useful for the synthesis of chiral alcohols. Also related are isolated nucleic acids encoding G. oxydans 2-ketoreductase enzymes, and enzyme fragments and variants thereof, as well as vectors and host cells comprising these nucleic acids. Further related are isolated G. oxydans 2-ketoreductase polypeptides, and fragments and variants thereof, and antibodies that specifically bind to G. oxydans 2-ketoreductase polypeptides, fragments, or variants. The invention also relates to methods of obtaining isolated G. oxydans 2-ketoreductase nucleic acids, polypeptides, and antibodies, and methods of using G. oxydans 2-ketoreductase in various reactions for industrial or pharmaceutical applications.

The invention relates to the reuse of shrimp and crab waste, specifically a method for simultaneously producing shrimp and crab xanthin and chitosan by using shrimp and crab waste, using the synergistic effect of Bacillus subtilis and Gluconobacter oxydans to add glucose to ferment Shrimp and crab skin and crab head are desalinated and deproteinized, and then fermented by Streptococcus thermophilus, Lactobacillus acidophilus, and Lactobacillus bulgaricus for one week to decalcify and decolorize, use chitin deacetylase to treat chitin, and use leather Glucosamine can be obtained by processing Plasmomonas Shigella, a lambs negative bacteria. During the fermentation process, there is no need to add a large amount of acid and alkali, which reduces environmental pollution, and microbial fermentation saves costs compared with enzyme fermentation, and the content of active ingredients obtained is higher, which realizes the complete utilization of active ingredients of raw materials, and is economically feasible.

The invention provides a method of fementing shrimp heads to prepare active substances, chitin and organic acidity calcium, wherein Bacillus licheniformis and Gluconobacter oxydans are utilized to ferment the shrimp heads to prepare the active substances, the chitin and the organic acidity calcium. The method utilizes synergistic effects of the Bacillus licheniformis and the Gluconobacter oxydans to ferment the shrimp head, is free of adding any acid and alkali, and meanwhile uses tap water to ferment, thereby greatly reducing production cost. The obtained broth is rich in shrimp incense, free of fishy smell and bitter taste, and simultaneously rich in amino acids, polyphenol, compound enzymes and a plurality of beneficial components, and can be used for producing flavourings, hydrolyzed protein products, functional foods, health products and other fields. By using the method provided by the invention, a plurality of active substances can be simultaneously separated and prepared from a batch of shrimp heads, such as hydrolyzed proteins rich in the amino acids, the polyphenol and a plurality of active substances; food-grade chitin; and calcium gluconate relatively appropriate for the body to absorb, thereby realizing comprehensive utilization of effective components in the raw materials.

The present invention relates to a strain HD385 producing L-erythrulose through microorganism fermentation, wherein the classification belongs to Acetobacteraceae (Gluconobacter) Gluconobacterkondonii, and the preservation number of the strain in the China General Microbiological Culture Collection Center is CGMCC No.8391. The present invention further provides a method for producing L-erythrulose by using the strain to ferment erythritol, wherein L-erythrulose can be significantly accumulated under an aerobic condition and can be up to 186.5 g / L, and the industrial production can be achieved.