Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving gluconobacter oxydans to produce 2-keto-L-gulonic acid

A technology of Gluconobacter oxydans and Gulonic acid, which is applied in the field of microbial fermentation, can solve the problems of increased cost, slow growth, and increased cost, and achieve the effects of reducing dosage, increasing growth speed, and reducing costs

Active Publication Date: 2009-12-16
TIANJIN UNIV
View PDF0 Cites 20 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mixed strains used in the mixed fermentation are Bacillus megaterium and Gluconobacter oxidans mixed bacteria fermentation, wherein Gluconobacter oxidans is an acid-producing bacterium, and Bacillus megaterium is an associated bacterium. Glycobacterium, the growth is extremely slow, and the acid production efficiency is low. When Bacillus megaterium is added, the growth rate of Gluconobacter oxydans can be increased, and the acid production can be increased. However, its addition can cause waste of nutrients in the fermentation medium. The cost is increased, and at the same time, because of its high concentration in the fermentation broth, the cost of processing the mixed-bacteria fermentation broth process will also increase greatly, and the mixed-bacteria metabolites are complex in composition and difficult to post-process
pollute the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving gluconobacter oxydans to produce 2-keto-L-gulonic acid
  • Method for improving gluconobacter oxydans to produce 2-keto-L-gulonic acid
  • Method for improving gluconobacter oxydans to produce 2-keto-L-gulonic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The method for improving the production of 2-keto-L-gulonic acid by Gluconobacter oxydans comprises the following steps:

[0032] (1) Seed cultivation:

[0033] Weigh: L-sorbose 20g, corn steep liquor 3g, beef extract 3g, yeast extract powder 3g, urea 1g, peptone 10g, KH 2 PO 4 1g, MgSO 4 0.2g, CaCO 3 Add 1 g of water to 1 L, adjust the pH to 6.8, and sterilize at 121°C for 20 minutes to make a seed medium;

[0034] Inoculate Gluconobacter oxydans (Gluconobacter oxydans) CGMCC NO.1.110 on the slant into the seed medium, shake at 30°C and 220r / min on a shaker, and cultivate for 36 hours to prepare a seed culture solution;

[0035] (2) Fermentation:

[0036] Weighing: L-sorbose 80g, corn steep liquor 20g, urea 12g, KH 2 PO 4 1g, MgSO 4 0.5g, CaCO 3 Add 5 g of water to 1 L, adjust the pH to 7.0, and sterilize at 121°C for 20 minutes to make a fermentation medium;

[0037] The seed culture solution was inserted into the fermentation medium at a ratio of 10% by vol...

Embodiment 2

[0040] The method for improving the production of 2-keto-L-gulonic acid by Gluconobacter oxydans comprises the following steps:

[0041] (1) Seed cultivation:

[0042] Weigh: L-sorbose 10g, corn steep liquor 2g, beef extract 2g, yeast extract powder 2g, urea 0.5g, peptone 2g, KH 2 PO 4 0.5g, MgSO 4 0.1g, CaCO 3 Add 0.5 g of water to 1 L, adjust the pH to 7.0, and sterilize at 121°C for 20 minutes to make a seed medium;

[0043] Inoculate Gluconobacter oxydans (Gluconobacter oxydans) CGMCC NO.1.110 on the slant into the seed medium, shake at 28°C and 160r / min on a shaker, and cultivate for 48 hours to prepare a seed culture solution;

[0044] (2) Fermentation:

[0045] Weighing: L-sorbose 40g, corn steep liquor 10g, urea 10g, KH 2 PO 4 0.5g, MgSO 4 0.2g, CaCO 3 Add 1 g of water to 1 L, adjust the pH to 7.5, and sterilize at 121°C for 20 minutes to make a fermentation medium;

[0046] The seed culture solution was inserted into the fermentation medium at a ratio of 5% ...

Embodiment 3

[0049] The method for improving the production of 2-keto-L-gulonic acid by Gluconobacter oxydans comprises the following steps:

[0050] (1) Seed cultivation:

[0051] Weighing: 50g of L-sorbose, 10g of corn steep liquor, 10g of beef extract, 10g of yeast extract powder, 5g of urea, 10g of peptone, KH 2 PO 4 5g, MgSO 4 0.7g, CaCO 3 Add 5 g of water to 1 L, adjust the pH to 6.5, and sterilize at 121°C for 20 minutes to make a seed medium;

[0052] Inoculate Gluconobacter oxydans (Gluconobacter oxydans) CGMCC NO.1.110 on the slant into the seed medium, shake at 35°C and 250r / min on a shaker, and cultivate for 24 hours to prepare a seed culture solution;

[0053] (2) Fermentation:

[0054] Weighing: L-sorbose 120g, corn steep liquor 50g, urea 25g, KH 2 PO 4 3g, MgSO 4 1.2g, CaCO 3 Add 10g of water to 1L, adjust the pH to 6.5, and sterilize at 121°C for 20 minutes to make a fermentation medium;

[0055] The seed culture solution was inserted into the fermentation medium ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving gluconobacter oxydans to produce 2-keto-L-gulonic acid, which comprises the following steps: (1) seed culture, namely inoculating bevel gluconobacter oxydans into a seed culture medium to form seed culture solution; and (2) fermentation, namely inoculating the seed culture solution into a fermentation culture medium, oscillating the mixture in a shaking bed, carrying out fermentation culture for 48 to 96 hours, and adding a sulfhydryl compound into the mixture at any moment of the fermentation culture in a range of 0 to 36 hours to make the final concentration of sulfhydryl between 0.1 and 30mM. The method can improve the growth speed of the gluconobacter oxydans and the efficiency for producing the 2-keto-L-gulonic acid, and achieve the aim of partially replacing the companion effect of companion fungus so as to reduce the using amount of bacillus megaterium of the companion fungus, reduce the cost of the culture medium and reduce the pollution.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, in particular to a method for improving the production of 2-keto-L-gulonic acid by Gluconobacter oxydans. Background technique [0002] Vitamin C is a trace water-soluble vitamin necessary for the nutrition and growth of the body, and plays an important role in anti-oxidation and maintaining metabolic balance. Vitamin C can be used as medicine, health products, food additives and cosmetic nutrients, its application scope is expanding and the market is stable. [0003] At present, the method of producing vitamin C in my country is a "two-step fermentation method". The first step of fermentation uses Acetobacter black to convert sorbitol into L-sorbose. Precursor 2-keto-L-gulonic acid. The mixed strain used in the mixed fermentation is Bacillus megaterium and Gluconobacter oxidans mixed bacteria fermentation, wherein, Gluconobacter oxidans is an acid-producing bacterium, Bacillus megaterium i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/60C12R1/01
Inventor 元英进仪宏王丽丽周剑马倩薛佳
Owner TIANJIN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com